Reduced facilitation and vesicular uptake in crustacean and mammalian neuromuscular junction by T-588, a neuroprotective compound

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Bath application of compound T-588, a neuroprotective agent, reduced paired-pulse and repetitive-pulse facilitation at mammalian and crustacean neuromuscular junctions. In addition, it reduced voltage-gated sodium and potassium currents in a use-dependent fashion, but had only a small effect on the...

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Autores principales:	Hirata, K., Nakagawa, M., Urbano, F.J., Rosato-Siri, M.D., Moreira, J.E., Uchitel, O.D., Sugimori, M., Llinás, R.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	1999
Materias:	FM 1-43 Two-photon microscopy Ultrastructure Voltage clamp 1 (benzo[b]thien 5 yl) 2 (2 diethylaminoethoxy)ethanol acetylcholine release animal cell article calcium conductance cell death cell ultrastructure drug effect facilitation mouse nerve cell neuromuscular synapse neuroprotection nonhuman potassium current priority journal sodium current synaptic transmission Action Potentials Animals Astacoidea Calcium Diethylamines Endocytosis Glutamic Acid Male Mice Neuromuscular Junction Neuroprotective Agents Potassium Channels Sodium Channels Synaptic Transmission Synaptic Vesicles Thiophenes Animalia Crustacea Mammalia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00278424_v96_n25_p14588_Hirata
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_00278424_v96_n25_p14588_Hirata
record_format	dspace
spelling	paperaa:paper_00278424_v96_n25_p14588_Hirata2023-06-12T16:45:22Z Reduced facilitation and vesicular uptake in crustacean and mammalian neuromuscular junction by T-588, a neuroprotective compound Proc. Natl. Acad. Sci. U. S. A. 1999;96(25):14588-14593 Hirata, K. Nakagawa, M. Urbano, F.J. Rosato-Siri, M.D. Moreira, J.E. Uchitel, O.D. Sugimori, M. Llinás, R. FM 1-43 Two-photon microscopy Ultrastructure Voltage clamp 1 (benzo[b]thien 5 yl) 2 (2 diethylaminoethoxy)ethanol acetylcholine release animal cell article calcium conductance cell death cell ultrastructure drug effect facilitation mouse nerve cell neuromuscular synapse neuroprotection nonhuman potassium current priority journal sodium current synaptic transmission Action Potentials Animals Astacoidea Calcium Diethylamines Endocytosis Glutamic Acid Male Mice Neuromuscular Junction Neuroprotective Agents Potassium Channels Sodium Channels Synaptic Transmission Synaptic Vesicles Thiophenes Animalia Astacoidea Crustacea Mammalia Bath application of compound T-588, a neuroprotective agent, reduced paired-pulse and repetitive-pulse facilitation at mammalian and crustacean neuromuscular junctions. In addition, it reduced voltage-gated sodium and potassium currents in a use-dependent fashion, but had only a small effect on the presynaptic Ca 2+ conductance. By contrast, it blocked FM 1-43 vesicular uptake but not its release, in both species. Postsynaptically, T-588 reduced acetylcholine currents at the mammalian junction in a voltage-independent manner, but had no effect on the crayfish glutamate junction. All of these effects were rapidly reversible and were observed at concentrations close to the compound's acute protective level. We propose that this set of mechanisms, which reduces high-frequency synaptic transmission, is an important contributory factor in the neuroprotective action of T-588. Fil:Rosato-Siri, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Uchitel, O.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1999 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00278424_v96_n25_p14588_Hirata
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	FM 1-43 Two-photon microscopy Ultrastructure Voltage clamp 1 (benzo[b]thien 5 yl) 2 (2 diethylaminoethoxy)ethanol acetylcholine release animal cell article calcium conductance cell death cell ultrastructure drug effect facilitation mouse nerve cell neuromuscular synapse neuroprotection nonhuman potassium current priority journal sodium current synaptic transmission Action Potentials Animals Astacoidea Calcium Diethylamines Endocytosis Glutamic Acid Male Mice Neuromuscular Junction Neuroprotective Agents Potassium Channels Sodium Channels Synaptic Transmission Synaptic Vesicles Thiophenes Animalia Astacoidea Crustacea Mammalia
spellingShingle	FM 1-43 Two-photon microscopy Ultrastructure Voltage clamp 1 (benzo[b]thien 5 yl) 2 (2 diethylaminoethoxy)ethanol acetylcholine release animal cell article calcium conductance cell death cell ultrastructure drug effect facilitation mouse nerve cell neuromuscular synapse neuroprotection nonhuman potassium current priority journal sodium current synaptic transmission Action Potentials Animals Astacoidea Calcium Diethylamines Endocytosis Glutamic Acid Male Mice Neuromuscular Junction Neuroprotective Agents Potassium Channels Sodium Channels Synaptic Transmission Synaptic Vesicles Thiophenes Animalia Astacoidea Crustacea Mammalia Hirata, K. Nakagawa, M. Urbano, F.J. Rosato-Siri, M.D. Moreira, J.E. Uchitel, O.D. Sugimori, M. Llinás, R. Reduced facilitation and vesicular uptake in crustacean and mammalian neuromuscular junction by T-588, a neuroprotective compound
topic_facet	FM 1-43 Two-photon microscopy Ultrastructure Voltage clamp 1 (benzo[b]thien 5 yl) 2 (2 diethylaminoethoxy)ethanol acetylcholine release animal cell article calcium conductance cell death cell ultrastructure drug effect facilitation mouse nerve cell neuromuscular synapse neuroprotection nonhuman potassium current priority journal sodium current synaptic transmission Action Potentials Animals Astacoidea Calcium Diethylamines Endocytosis Glutamic Acid Male Mice Neuromuscular Junction Neuroprotective Agents Potassium Channels Sodium Channels Synaptic Transmission Synaptic Vesicles Thiophenes Animalia Astacoidea Crustacea Mammalia
description	Bath application of compound T-588, a neuroprotective agent, reduced paired-pulse and repetitive-pulse facilitation at mammalian and crustacean neuromuscular junctions. In addition, it reduced voltage-gated sodium and potassium currents in a use-dependent fashion, but had only a small effect on the presynaptic Ca 2+ conductance. By contrast, it blocked FM 1-43 vesicular uptake but not its release, in both species. Postsynaptically, T-588 reduced acetylcholine currents at the mammalian junction in a voltage-independent manner, but had no effect on the crayfish glutamate junction. All of these effects were rapidly reversible and were observed at concentrations close to the compound's acute protective level. We propose that this set of mechanisms, which reduces high-frequency synaptic transmission, is an important contributory factor in the neuroprotective action of T-588.
format	Artículo Artículo publishedVersion
author	Hirata, K. Nakagawa, M. Urbano, F.J. Rosato-Siri, M.D. Moreira, J.E. Uchitel, O.D. Sugimori, M. Llinás, R.
author_facet	Hirata, K. Nakagawa, M. Urbano, F.J. Rosato-Siri, M.D. Moreira, J.E. Uchitel, O.D. Sugimori, M. Llinás, R.
author_sort	Hirata, K.
title	Reduced facilitation and vesicular uptake in crustacean and mammalian neuromuscular junction by T-588, a neuroprotective compound
title_short	Reduced facilitation and vesicular uptake in crustacean and mammalian neuromuscular junction by T-588, a neuroprotective compound
title_full	Reduced facilitation and vesicular uptake in crustacean and mammalian neuromuscular junction by T-588, a neuroprotective compound
title_fullStr	Reduced facilitation and vesicular uptake in crustacean and mammalian neuromuscular junction by T-588, a neuroprotective compound
title_full_unstemmed	Reduced facilitation and vesicular uptake in crustacean and mammalian neuromuscular junction by T-588, a neuroprotective compound
title_sort	reduced facilitation and vesicular uptake in crustacean and mammalian neuromuscular junction by t-588, a neuroprotective compound
publishDate	1999
url	http://hdl.handle.net/20.500.12110/paper_00278424_v96_n25_p14588_Hirata
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Reduced facilitation and vesicular uptake in crustacean and mammalian neuromuscular junction by T-588, a neuroprotective compound

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