Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells

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Oligonucleotides (ODNs) of the PyNTTTTGT class directly stimulate B lymphocytes and plasmacytoid dendritic cells of the immune system of primates. Here we investigated the ability of the PyNTTTTGT ODN prototype IMT504 to regulate the expression of surface molecules and apoptosis in human B-chronic l...

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Detalles Bibliográficos
Autores principales: Rodrigues, J.M., Elias, F., Montaner, A., Flo, J., Lopez, R.A., Zorzopulos, J., Franco, R.J., Lenial, S.P., Lopez Salón, M., Pirpignani, M.L., Solimano, J., Garay, G., Riveros, D., Fernandez, J., Cacchione, R., Dupont, J.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2006
Materias:
Chronic lymphocytic leukemia
IL-2
Immunogenic phenotype
Immunostimulatory ODN
VH genes mutation
B7 antigen
CD40 antigen
CD86 antigen
immunoglobulin
interleukin 2 receptor alpha
oligodeoxynucleotide
recombinant interleukin 2
adult
aged
apoptosis
article
B lymphocyte activation
cancer growth
chronic lymphatic leukemia
clinical article
controlled study
dendritic cell
disease marker
female
gene mutation
human
human cell
immune system
immunogenicity
in vitro study
incubation time
leukemia cell
male
mixed lymphocyte reaction
phenotype
primate
protein expression
T lymphocyte activation
upregulation
Aged
Antigens, CD
Apoptosis
Female
Humans
Immunophenotyping
Interleukin-2
Leukemia, B-Cell, Chronic
Male
Middle Aged
Mutation
Oligodeoxyribonucleotides
Phenotype
Polymerase Chain Reaction
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00257680_v66_n1_p9_Rodrigues
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Oligonucleotides (ODNs) of the PyNTTTTGT class directly stimulate B lymphocytes and plasmacytoid dendritic cells of the immune system of primates. Here we investigated the ability of the PyNTTTTGT ODN prototype IMT504 to regulate the expression of surface molecules and apoptosis in human B-chronic lymphocytic leukemia (CLL) cells. The surface molecules CD25, CD40, CD80 and CD86 were up-regulated upon incubation of the B-CLL cells with IMT504. Co-stimulation with IL-2 resulted in further up-regulation. IMT504-activated B-CLL cells were also good stimulators of T cells in allogeneic mixed lymphocyte reactions and co-stimulation with IL-2 improved this stimulation capacity. Apoptosis of the B-CLL cells in vitro was also stimulated by incubation with IMT504. In this case, co-stimulation with IL-2 was not significant. Furthermore, B-CLL cells of all the patients studied developed an immunogenic phenotype and entered stimulated apoptosis upon in vitro incubation with IMT504 independently of the mutational status of their 1gVH genes, becoming a good marker for tumor progression.

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