Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells

Mostrar todas las versiones(3)

Oligonucleotides (ODNs) of the PyNTTTTGT class directly stimulate B lymphocytes and plasmacytoid dendritic cells of the immune system of primates. Here we investigated the ability of the PyNTTTTGT ODN prototype IMT504 to regulate the expression of surface molecules and apoptosis in human B-chronic l...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rodrigues, J.M., Elias, F., Montaner, A., Flo, J., Lopez, R.A., Zorzopulos, J., Franco, R.J., Lenial, S.P., Lopez Salón, M., Pirpignani, M.L., Solimano, J., Garay, G., Riveros, D., Fernandez, J., Cacchione, R., Dupont, J.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2006
Materias:	Chronic lymphocytic leukemia IL-2 Immunogenic phenotype Immunostimulatory ODN VH genes mutation B7 antigen CD40 antigen CD86 antigen immunoglobulin interleukin 2 receptor alpha oligodeoxynucleotide recombinant interleukin 2 adult aged apoptosis article B lymphocyte activation cancer growth chronic lymphatic leukemia clinical article controlled study dendritic cell disease marker female gene mutation human human cell immune system immunogenicity in vitro study incubation time leukemia cell male mixed lymphocyte reaction phenotype primate protein expression T lymphocyte activation upregulation Aged Antigens, CD Apoptosis Female Humans Immunophenotyping Interleukin-2 Leukemia, B-Cell, Chronic Male Middle Aged Mutation Oligodeoxyribonucleotides Phenotype Polymerase Chain Reaction
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00257680_v66_n1_p9_Rodrigues
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_00257680_v66_n1_p9_Rodrigues
record_format	dspace
spelling	paperaa:paper_00257680_v66_n1_p9_Rodrigues2023-06-12T16:45:10Z Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells Medicina (Argentina) 2006;66(1):9-16 Rodrigues, J.M. Elias, F. Montaner, A. Flo, J. Lopez, R.A. Zorzopulos, J. Franco, R.J. Lenial, S.P. Lopez Salón, M. Pirpignani, M.L. Solimano, J. Garay, G. Riveros, D. Fernandez, J. Cacchione, R. Dupont, J. Chronic lymphocytic leukemia IL-2 Immunogenic phenotype Immunostimulatory ODN VH genes mutation B7 antigen CD40 antigen CD86 antigen immunoglobulin interleukin 2 receptor alpha oligodeoxynucleotide recombinant interleukin 2 adult aged apoptosis article B lymphocyte activation cancer growth chronic lymphatic leukemia clinical article controlled study dendritic cell disease marker female gene mutation human human cell immune system immunogenicity in vitro study incubation time leukemia cell male mixed lymphocyte reaction phenotype primate protein expression T lymphocyte activation upregulation Aged Antigens, CD Apoptosis Female Humans Immunophenotyping Interleukin-2 Leukemia, B-Cell, Chronic Male Middle Aged Mutation Oligodeoxyribonucleotides Phenotype Polymerase Chain Reaction Oligonucleotides (ODNs) of the PyNTTTTGT class directly stimulate B lymphocytes and plasmacytoid dendritic cells of the immune system of primates. Here we investigated the ability of the PyNTTTTGT ODN prototype IMT504 to regulate the expression of surface molecules and apoptosis in human B-chronic lymphocytic leukemia (CLL) cells. The surface molecules CD25, CD40, CD80 and CD86 were up-regulated upon incubation of the B-CLL cells with IMT504. Co-stimulation with IL-2 resulted in further up-regulation. IMT504-activated B-CLL cells were also good stimulators of T cells in allogeneic mixed lymphocyte reactions and co-stimulation with IL-2 improved this stimulation capacity. Apoptosis of the B-CLL cells in vitro was also stimulated by incubation with IMT504. In this case, co-stimulation with IL-2 was not significant. Furthermore, B-CLL cells of all the patients studied developed an immunogenic phenotype and entered stimulated apoptosis upon in vitro incubation with IMT504 independently of the mutational status of their 1gVH genes, becoming a good marker for tumor progression. Fil:Elias, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00257680_v66_n1_p9_Rodrigues
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	Chronic lymphocytic leukemia IL-2 Immunogenic phenotype Immunostimulatory ODN VH genes mutation B7 antigen CD40 antigen CD86 antigen immunoglobulin interleukin 2 receptor alpha oligodeoxynucleotide recombinant interleukin 2 adult aged apoptosis article B lymphocyte activation cancer growth chronic lymphatic leukemia clinical article controlled study dendritic cell disease marker female gene mutation human human cell immune system immunogenicity in vitro study incubation time leukemia cell male mixed lymphocyte reaction phenotype primate protein expression T lymphocyte activation upregulation Aged Antigens, CD Apoptosis Female Humans Immunophenotyping Interleukin-2 Leukemia, B-Cell, Chronic Male Middle Aged Mutation Oligodeoxyribonucleotides Phenotype Polymerase Chain Reaction
spellingShingle	Chronic lymphocytic leukemia IL-2 Immunogenic phenotype Immunostimulatory ODN VH genes mutation B7 antigen CD40 antigen CD86 antigen immunoglobulin interleukin 2 receptor alpha oligodeoxynucleotide recombinant interleukin 2 adult aged apoptosis article B lymphocyte activation cancer growth chronic lymphatic leukemia clinical article controlled study dendritic cell disease marker female gene mutation human human cell immune system immunogenicity in vitro study incubation time leukemia cell male mixed lymphocyte reaction phenotype primate protein expression T lymphocyte activation upregulation Aged Antigens, CD Apoptosis Female Humans Immunophenotyping Interleukin-2 Leukemia, B-Cell, Chronic Male Middle Aged Mutation Oligodeoxyribonucleotides Phenotype Polymerase Chain Reaction Rodrigues, J.M. Elias, F. Montaner, A. Flo, J. Lopez, R.A. Zorzopulos, J. Franco, R.J. Lenial, S.P. Lopez Salón, M. Pirpignani, M.L. Solimano, J. Garay, G. Riveros, D. Fernandez, J. Cacchione, R. Dupont, J. Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells
topic_facet	Chronic lymphocytic leukemia IL-2 Immunogenic phenotype Immunostimulatory ODN VH genes mutation B7 antigen CD40 antigen CD86 antigen immunoglobulin interleukin 2 receptor alpha oligodeoxynucleotide recombinant interleukin 2 adult aged apoptosis article B lymphocyte activation cancer growth chronic lymphatic leukemia clinical article controlled study dendritic cell disease marker female gene mutation human human cell immune system immunogenicity in vitro study incubation time leukemia cell male mixed lymphocyte reaction phenotype primate protein expression T lymphocyte activation upregulation Aged Antigens, CD Apoptosis Female Humans Immunophenotyping Interleukin-2 Leukemia, B-Cell, Chronic Male Middle Aged Mutation Oligodeoxyribonucleotides Phenotype Polymerase Chain Reaction
description	Oligonucleotides (ODNs) of the PyNTTTTGT class directly stimulate B lymphocytes and plasmacytoid dendritic cells of the immune system of primates. Here we investigated the ability of the PyNTTTTGT ODN prototype IMT504 to regulate the expression of surface molecules and apoptosis in human B-chronic lymphocytic leukemia (CLL) cells. The surface molecules CD25, CD40, CD80 and CD86 were up-regulated upon incubation of the B-CLL cells with IMT504. Co-stimulation with IL-2 resulted in further up-regulation. IMT504-activated B-CLL cells were also good stimulators of T cells in allogeneic mixed lymphocyte reactions and co-stimulation with IL-2 improved this stimulation capacity. Apoptosis of the B-CLL cells in vitro was also stimulated by incubation with IMT504. In this case, co-stimulation with IL-2 was not significant. Furthermore, B-CLL cells of all the patients studied developed an immunogenic phenotype and entered stimulated apoptosis upon in vitro incubation with IMT504 independently of the mutational status of their 1gVH genes, becoming a good marker for tumor progression.
format	Artículo Artículo publishedVersion
author	Rodrigues, J.M. Elias, F. Montaner, A. Flo, J. Lopez, R.A. Zorzopulos, J. Franco, R.J. Lenial, S.P. Lopez Salón, M. Pirpignani, M.L. Solimano, J. Garay, G. Riveros, D. Fernandez, J. Cacchione, R. Dupont, J.
author_facet	Rodrigues, J.M. Elias, F. Montaner, A. Flo, J. Lopez, R.A. Zorzopulos, J. Franco, R.J. Lenial, S.P. Lopez Salón, M. Pirpignani, M.L. Solimano, J. Garay, G. Riveros, D. Fernandez, J. Cacchione, R. Dupont, J.
author_sort	Rodrigues, J.M.
title	Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells
title_short	Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells
title_full	Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells
title_fullStr	Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells
title_full_unstemmed	Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells
title_sort	oligonucleotide imt504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells
publishDate	2006
url	http://hdl.handle.net/20.500.12110/paper_00257680_v66_n1_p9_Rodrigues
work_keys_str_mv	AT rodriguesjm oligonucleotideimt504inducesanimmunogenicphenotypeandapoptosisinchroniclymphocyticleukemiacells AT eliasf oligonucleotideimt504inducesanimmunogenicphenotypeandapoptosisinchroniclymphocyticleukemiacells AT montanera oligonucleotideimt504inducesanimmunogenicphenotypeandapoptosisinchroniclymphocyticleukemiacells AT floj oligonucleotideimt504inducesanimmunogenicphenotypeandapoptosisinchroniclymphocyticleukemiacells AT lopezra oligonucleotideimt504inducesanimmunogenicphenotypeandapoptosisinchroniclymphocyticleukemiacells AT zorzopulosj oligonucleotideimt504inducesanimmunogenicphenotypeandapoptosisinchroniclymphocyticleukemiacells AT francorj oligonucleotideimt504inducesanimmunogenicphenotypeandapoptosisinchroniclymphocyticleukemiacells AT lenialsp oligonucleotideimt504inducesanimmunogenicphenotypeandapoptosisinchroniclymphocyticleukemiacells AT lopezsalonm oligonucleotideimt504inducesanimmunogenicphenotypeandapoptosisinchroniclymphocyticleukemiacells AT pirpignaniml oligonucleotideimt504inducesanimmunogenicphenotypeandapoptosisinchroniclymphocyticleukemiacells AT solimanoj oligonucleotideimt504inducesanimmunogenicphenotypeandapoptosisinchroniclymphocyticleukemiacells AT garayg oligonucleotideimt504inducesanimmunogenicphenotypeandapoptosisinchroniclymphocyticleukemiacells AT riverosd oligonucleotideimt504inducesanimmunogenicphenotypeandapoptosisinchroniclymphocyticleukemiacells AT fernandezj oligonucleotideimt504inducesanimmunogenicphenotypeandapoptosisinchroniclymphocyticleukemiacells AT cacchioner oligonucleotideimt504inducesanimmunogenicphenotypeandapoptosisinchroniclymphocyticleukemiacells AT dupontj oligonucleotideimt504inducesanimmunogenicphenotypeandapoptosisinchroniclymphocyticleukemiacells
_version_	1769810381745684480

Oligonucleotide IMT504 induces an immunogenic phenotype and apoptosis in chronic lymphocytic leukemia cells

Ejemplares similares