Contribution of Ca2+ calmodulin-dependent protein kinase II and mitogen-activated protein kinase kinase to neural activity-induced neurite outgrowth and survival of cerebellar granule cells

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In this report we describe our studies on intracellular signals that mediate neurite outgrowth and long-term survival of cerebellar granule cells. The effect of voltage-gated calcium channel activation on neurite complexity was evaluated in cultured cerebellar granule cells grown for 48 h at low den...

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Autores principales:	Borodinsky, L.N., Coso, O.A., Fiszman, M.L.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2002
Materias:	CaMKII and MEK1 pathways Cerebellar granule cells Fractal dimension Neural activity Neurite outgrowth Neuronal survival calcium channel calcium ion mitogen activated protein kinase 1 mitogen activated protein kinase kinase potassium chloride protein kinase (calcium,calmodulin) II calcium calcium channel blocking agent calmodulin dependent protein kinase ii calmodulin-dependent protein kinase II enzyme inhibitor mitogen activated protein kinase mitogen activated protein kinase 3 mitogen activated protein kinase kinase 1 protein kinase (calcium,calmodulin) protein serine threonine kinase animal cell article calcium cell level calcium signaling calcium transport cell survival controlled study fractal analysis granule cell nerve fiber growth nonhuman phenotype priority journal rat signal transduction animal cell culture cell differentiation cerebellum culture medium cytology drug antagonism drug effect enzyme activation enzymology metabolism nerve cell neurite physiology Sprague Dawley rat time Animalia Animals Ca(2+)-Calmodulin Dependent Protein Kinase Calcium Calcium Channel Blockers Calcium Channels Cell Differentiation Cell Survival Cells, Cultured Cerebellum Culture Media, Serum-Free Enzyme Activation Enzyme Inhibitors MAP Kinase Kinase 1 Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinase Kinases Mitogen-Activated Protein Kinases Neurites Neurons Protein-Serine-Threonine Kinases Rats Rats, Sprague-Dawley Signal Transduction Time Factors
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00223042_v80_n6_p1062_Borodinsky
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paperaa:paper_00223042_v80_n6_p1062_Borodinsky2023-06-12T16:44:40Z Contribution of Ca2+ calmodulin-dependent protein kinase II and mitogen-activated protein kinase kinase to neural activity-induced neurite outgrowth and survival of cerebellar granule cells J. Neurochem. 2002;80(6):1062-1070 Borodinsky, L.N. Coso, O.A. Fiszman, M.L. CaMKII and MEK1 pathways Cerebellar granule cells Fractal dimension Neural activity Neurite outgrowth Neuronal survival calcium channel calcium ion mitogen activated protein kinase 1 mitogen activated protein kinase kinase potassium chloride protein kinase (calcium,calmodulin) II calcium calcium channel calcium channel blocking agent calmodulin dependent protein kinase ii calmodulin-dependent protein kinase II enzyme inhibitor mitogen activated protein kinase mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase kinase mitogen activated protein kinase kinase 1 protein kinase (calcium,calmodulin) protein serine threonine kinase animal cell article calcium cell level calcium signaling calcium transport cell survival controlled study fractal analysis granule cell nerve fiber growth nonhuman phenotype priority journal rat signal transduction animal cell culture cell differentiation cell survival cerebellum culture medium cytology drug antagonism drug effect enzyme activation enzymology metabolism nerve cell neurite physiology Sprague Dawley rat time Animalia Animals Ca(2+)-Calmodulin Dependent Protein Kinase Calcium Calcium Channel Blockers Calcium Channels Cell Differentiation Cell Survival Cells, Cultured Cerebellum Culture Media, Serum-Free Enzyme Activation Enzyme Inhibitors MAP Kinase Kinase 1 Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinase Kinases Mitogen-Activated Protein Kinases Neurites Neurons Protein-Serine-Threonine Kinases Rats Rats, Sprague-Dawley Signal Transduction Time Factors In this report we describe our studies on intracellular signals that mediate neurite outgrowth and long-term survival of cerebellar granule cells. The effect of voltage-gated calcium channel activation on neurite complexity was evaluated in cultured cerebellar granule cells grown for 48 h at low density; the parameter measured was the fractal dimension of the cell. We explored the contribution of two intracellular pathways, Ca2+ calmodulin-dependent protein kinase II and mitogen-activated protein kinase kinase (MEK1), to the effects of high [K+]e under serum-free conditions. We found that 25 mM KCI (25K) induced an increase in calcium influx through L subtype channels. In neurones grown for 24-48 h under low-density conditions, the activation of these channels induced neurite outgrowth through the activation of Ca2+ calmodulin-dependent protein kinase II. This also produced an increase in long-term neuronal survival with a partial contribution from the MEK1 pathway. We also found that the addition of 25K increased the levels of the phosphorylated forms of Ca2+ calmodulin-dependent protein kinase II and of the extracellular signal-regulated kinases 1 and 2. Neuronal survival under resting conditions is supported by the MEK1 pathway. We conclude that intracellular calcium oscillations can triggered different biological effects depending on the stage of maturation of the neuronal phenotype. Ca2+ calmodulin-dependent protein kinase II activation determines the growth of neurites and the development of neuronal complexity. Fil:Borodinsky, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coso, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00223042_v80_n6_p1062_Borodinsky
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	CaMKII and MEK1 pathways Cerebellar granule cells Fractal dimension Neural activity Neurite outgrowth Neuronal survival calcium channel calcium ion mitogen activated protein kinase 1 mitogen activated protein kinase kinase potassium chloride protein kinase (calcium,calmodulin) II calcium calcium channel calcium channel blocking agent calmodulin dependent protein kinase ii calmodulin-dependent protein kinase II enzyme inhibitor mitogen activated protein kinase mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase kinase mitogen activated protein kinase kinase 1 protein kinase (calcium,calmodulin) protein serine threonine kinase animal cell article calcium cell level calcium signaling calcium transport cell survival controlled study fractal analysis granule cell nerve fiber growth nonhuman phenotype priority journal rat signal transduction animal cell culture cell differentiation cell survival cerebellum culture medium cytology drug antagonism drug effect enzyme activation enzymology metabolism nerve cell neurite physiology Sprague Dawley rat time Animalia Animals Ca(2+)-Calmodulin Dependent Protein Kinase Calcium Calcium Channel Blockers Calcium Channels Cell Differentiation Cell Survival Cells, Cultured Cerebellum Culture Media, Serum-Free Enzyme Activation Enzyme Inhibitors MAP Kinase Kinase 1 Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinase Kinases Mitogen-Activated Protein Kinases Neurites Neurons Protein-Serine-Threonine Kinases Rats Rats, Sprague-Dawley Signal Transduction Time Factors
spellingShingle	CaMKII and MEK1 pathways Cerebellar granule cells Fractal dimension Neural activity Neurite outgrowth Neuronal survival calcium channel calcium ion mitogen activated protein kinase 1 mitogen activated protein kinase kinase potassium chloride protein kinase (calcium,calmodulin) II calcium calcium channel calcium channel blocking agent calmodulin dependent protein kinase ii calmodulin-dependent protein kinase II enzyme inhibitor mitogen activated protein kinase mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase kinase mitogen activated protein kinase kinase 1 protein kinase (calcium,calmodulin) protein serine threonine kinase animal cell article calcium cell level calcium signaling calcium transport cell survival controlled study fractal analysis granule cell nerve fiber growth nonhuman phenotype priority journal rat signal transduction animal cell culture cell differentiation cell survival cerebellum culture medium cytology drug antagonism drug effect enzyme activation enzymology metabolism nerve cell neurite physiology Sprague Dawley rat time Animalia Animals Ca(2+)-Calmodulin Dependent Protein Kinase Calcium Calcium Channel Blockers Calcium Channels Cell Differentiation Cell Survival Cells, Cultured Cerebellum Culture Media, Serum-Free Enzyme Activation Enzyme Inhibitors MAP Kinase Kinase 1 Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinase Kinases Mitogen-Activated Protein Kinases Neurites Neurons Protein-Serine-Threonine Kinases Rats Rats, Sprague-Dawley Signal Transduction Time Factors Borodinsky, L.N. Coso, O.A. Fiszman, M.L. Contribution of Ca2+ calmodulin-dependent protein kinase II and mitogen-activated protein kinase kinase to neural activity-induced neurite outgrowth and survival of cerebellar granule cells
topic_facet	CaMKII and MEK1 pathways Cerebellar granule cells Fractal dimension Neural activity Neurite outgrowth Neuronal survival calcium channel calcium ion mitogen activated protein kinase 1 mitogen activated protein kinase kinase potassium chloride protein kinase (calcium,calmodulin) II calcium calcium channel calcium channel blocking agent calmodulin dependent protein kinase ii calmodulin-dependent protein kinase II enzyme inhibitor mitogen activated protein kinase mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase kinase mitogen activated protein kinase kinase 1 protein kinase (calcium,calmodulin) protein serine threonine kinase animal cell article calcium cell level calcium signaling calcium transport cell survival controlled study fractal analysis granule cell nerve fiber growth nonhuman phenotype priority journal rat signal transduction animal cell culture cell differentiation cell survival cerebellum culture medium cytology drug antagonism drug effect enzyme activation enzymology metabolism nerve cell neurite physiology Sprague Dawley rat time Animalia Animals Ca(2+)-Calmodulin Dependent Protein Kinase Calcium Calcium Channel Blockers Calcium Channels Cell Differentiation Cell Survival Cells, Cultured Cerebellum Culture Media, Serum-Free Enzyme Activation Enzyme Inhibitors MAP Kinase Kinase 1 Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinase Kinases Mitogen-Activated Protein Kinases Neurites Neurons Protein-Serine-Threonine Kinases Rats Rats, Sprague-Dawley Signal Transduction Time Factors
description	In this report we describe our studies on intracellular signals that mediate neurite outgrowth and long-term survival of cerebellar granule cells. The effect of voltage-gated calcium channel activation on neurite complexity was evaluated in cultured cerebellar granule cells grown for 48 h at low density; the parameter measured was the fractal dimension of the cell. We explored the contribution of two intracellular pathways, Ca2+ calmodulin-dependent protein kinase II and mitogen-activated protein kinase kinase (MEK1), to the effects of high [K+]e under serum-free conditions. We found that 25 mM KCI (25K) induced an increase in calcium influx through L subtype channels. In neurones grown for 24-48 h under low-density conditions, the activation of these channels induced neurite outgrowth through the activation of Ca2+ calmodulin-dependent protein kinase II. This also produced an increase in long-term neuronal survival with a partial contribution from the MEK1 pathway. We also found that the addition of 25K increased the levels of the phosphorylated forms of Ca2+ calmodulin-dependent protein kinase II and of the extracellular signal-regulated kinases 1 and 2. Neuronal survival under resting conditions is supported by the MEK1 pathway. We conclude that intracellular calcium oscillations can triggered different biological effects depending on the stage of maturation of the neuronal phenotype. Ca2+ calmodulin-dependent protein kinase II activation determines the growth of neurites and the development of neuronal complexity.
format	Artículo Artículo publishedVersion
author	Borodinsky, L.N. Coso, O.A. Fiszman, M.L.
author_facet	Borodinsky, L.N. Coso, O.A. Fiszman, M.L.
author_sort	Borodinsky, L.N.
title	Contribution of Ca2+ calmodulin-dependent protein kinase II and mitogen-activated protein kinase kinase to neural activity-induced neurite outgrowth and survival of cerebellar granule cells
title_short	Contribution of Ca2+ calmodulin-dependent protein kinase II and mitogen-activated protein kinase kinase to neural activity-induced neurite outgrowth and survival of cerebellar granule cells
title_full	Contribution of Ca2+ calmodulin-dependent protein kinase II and mitogen-activated protein kinase kinase to neural activity-induced neurite outgrowth and survival of cerebellar granule cells
title_fullStr	Contribution of Ca2+ calmodulin-dependent protein kinase II and mitogen-activated protein kinase kinase to neural activity-induced neurite outgrowth and survival of cerebellar granule cells
title_full_unstemmed	Contribution of Ca2+ calmodulin-dependent protein kinase II and mitogen-activated protein kinase kinase to neural activity-induced neurite outgrowth and survival of cerebellar granule cells
title_sort	contribution of ca2+ calmodulin-dependent protein kinase ii and mitogen-activated protein kinase kinase to neural activity-induced neurite outgrowth and survival of cerebellar granule cells
publishDate	2002
url	http://hdl.handle.net/20.500.12110/paper_00223042_v80_n6_p1062_Borodinsky
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_version_	1769810276737089536

Contribution of Ca2+ calmodulin-dependent protein kinase II and mitogen-activated protein kinase kinase to neural activity-induced neurite outgrowth and survival of cerebellar granule cells

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