Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination

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The role of GAs in promoting seed germination is well known and experiments with seeds from different species have suggested the requirement of de novo synthesis of GAs upon imbibition for germination. There are also strong indications that the enhancement of GA synthesis is part of the mechanism th...

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Autores principales: Pérez-Flores, L., Carrari, F., Osuna-Fernández, R., Rodríguez, M.V., Enciso, S., Stanelloni, R., Sánchez, R.A., Bottini, R., Iusem, N.D., Benech-Arnold, R.L.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2003
Materias:
Dormancy
GA 20-oxidase
Germination
Gibberellins
Sorghum
Biosynthesis
Enzymes
Genes
Tissue
Seed germination
Seed
Amino Acid Sequence
Blotting, Southern
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Mixed Function Oxygenases
Molecular Sequence Data
Plant Growth Regulators
Poaceae
RNA, Messenger
Seeds
Sequence Alignment
Sequence Homology, Amino Acid
Species Specificity
Embryophyta
Eukaryota
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00220957_v54_n390_p2071_PerezFlores
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paperaa:paper_00220957_v54_n390_p2071_PerezFlores
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spelling paperaa:paper_00220957_v54_n390_p2071_PerezFlores2023-06-12T16:43:47Z Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination J. Exp. Bot. 2003;54(390):2071-2079 Pérez-Flores, L. Carrari, F. Osuna-Fernández, R. Rodríguez, M.V. Enciso, S. Stanelloni, R. Sánchez, R.A. Bottini, R. Iusem, N.D. Benech-Arnold, R.L. Dormancy GA 20-oxidase Germination Gibberellins Sorghum Biosynthesis Enzymes Genes Tissue Seed germination Seed Amino Acid Sequence Blotting, Southern Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Mixed Function Oxygenases Molecular Sequence Data Plant Growth Regulators Poaceae RNA, Messenger Seeds Sequence Alignment Sequence Homology, Amino Acid Species Specificity Embryophyta Eukaryota The role of GAs in promoting seed germination is well known and experiments with seeds from different species have suggested the requirement of de novo synthesis of GAs upon imbibition for germination. There are also strong indications that the enhancement of GA synthesis is part of the mechanism through which environmental signals (i.e. light) induce germination. Since along the GA biosynthetic pathway, oxidation at C-20 carried out by GA 20-oxidases is thought to be a site of regulation, a cDNA clone encoding a GA 20-oxidase was isolated from embryos of sorghum (SbGA 20ox). Expression analysis of this gene in embryos within imbibed caryopses with low dormancy showed detectable amounts of the specific mRNA early upon incubation, increasing thereafter. In contrast, it remained barely detectable in embryos from dormant caryopses. Changes in endogenous GA4 levels were in agreement with those of SbGA 20ox mRNA, suggesting that GA production might be regulated differentially at the level of transcription of this gene. The expression of SbGA 20ox was enhanced in incubated embryos isolated from either type of caryopses, illustrating a physiological control exerted by the surrounding seed tissues on gene expression. The results also show that ABA leads to a suppression of transcription of this gene. Fil:Carrari, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00220957_v54_n390_p2071_PerezFlores
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic Dormancy
GA 20-oxidase
Germination
Gibberellins
Sorghum
Biosynthesis
Enzymes
Genes
Tissue
Seed germination
Seed
Amino Acid Sequence
Blotting, Southern
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Mixed Function Oxygenases
Molecular Sequence Data
Plant Growth Regulators
Poaceae
RNA, Messenger
Seeds
Sequence Alignment
Sequence Homology, Amino Acid
Species Specificity
Embryophyta
Eukaryota
spellingShingle Dormancy
GA 20-oxidase
Germination
Gibberellins
Sorghum
Biosynthesis
Enzymes
Genes
Tissue
Seed germination
Seed
Amino Acid Sequence
Blotting, Southern
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Mixed Function Oxygenases
Molecular Sequence Data
Plant Growth Regulators
Poaceae
RNA, Messenger
Seeds
Sequence Alignment
Sequence Homology, Amino Acid
Species Specificity
Embryophyta
Eukaryota
Pérez-Flores, L.
Carrari, F.
Osuna-Fernández, R.
Rodríguez, M.V.
Enciso, S.
Stanelloni, R.
Sánchez, R.A.
Bottini, R.
Iusem, N.D.
Benech-Arnold, R.L.
Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination
topic_facet Dormancy
GA 20-oxidase
Germination
Gibberellins
Sorghum
Biosynthesis
Enzymes
Genes
Tissue
Seed germination
Seed
Amino Acid Sequence
Blotting, Southern
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Mixed Function Oxygenases
Molecular Sequence Data
Plant Growth Regulators
Poaceae
RNA, Messenger
Seeds
Sequence Alignment
Sequence Homology, Amino Acid
Species Specificity
Embryophyta
Eukaryota
description The role of GAs in promoting seed germination is well known and experiments with seeds from different species have suggested the requirement of de novo synthesis of GAs upon imbibition for germination. There are also strong indications that the enhancement of GA synthesis is part of the mechanism through which environmental signals (i.e. light) induce germination. Since along the GA biosynthetic pathway, oxidation at C-20 carried out by GA 20-oxidases is thought to be a site of regulation, a cDNA clone encoding a GA 20-oxidase was isolated from embryos of sorghum (SbGA 20ox). Expression analysis of this gene in embryos within imbibed caryopses with low dormancy showed detectable amounts of the specific mRNA early upon incubation, increasing thereafter. In contrast, it remained barely detectable in embryos from dormant caryopses. Changes in endogenous GA4 levels were in agreement with those of SbGA 20ox mRNA, suggesting that GA production might be regulated differentially at the level of transcription of this gene. The expression of SbGA 20ox was enhanced in incubated embryos isolated from either type of caryopses, illustrating a physiological control exerted by the surrounding seed tissues on gene expression. The results also show that ABA leads to a suppression of transcription of this gene.
format Artículo
Artículo
publishedVersion
author Pérez-Flores, L.
Carrari, F.
Osuna-Fernández, R.
Rodríguez, M.V.
Enciso, S.
Stanelloni, R.
Sánchez, R.A.
Bottini, R.
Iusem, N.D.
Benech-Arnold, R.L.
author_facet Pérez-Flores, L.
Carrari, F.
Osuna-Fernández, R.
Rodríguez, M.V.
Enciso, S.
Stanelloni, R.
Sánchez, R.A.
Bottini, R.
Iusem, N.D.
Benech-Arnold, R.L.
author_sort Pérez-Flores, L.
title Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination
title_short Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination
title_full Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination
title_fullStr Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination
title_full_unstemmed Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination
title_sort expression analysis of a ga 20-oxidase in embryos from two sorghum lines with contrasting dormancy: possible participation of this gene in the hormonal control of germination
publishDate 2003
url http://hdl.handle.net/20.500.12110/paper_00220957_v54_n390_p2071_PerezFlores
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