Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum
A genetic locus from Acetobacter xylinum involved in acetan polysaccharide synthesis has been characterized. The chromosomal region was identified by screening a genomic library of A. xylinum in a Xanthomonas campestris mutant defective in xanthan polysaccharide synthesis. The A. xylinum cosmid clon...
Guardado en:
Autores principales: | , |
---|---|
Formato: | Artículo publishedVersion |
Lenguaje: | Inglés |
Publicado: |
1996
|
Materias: | |
Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00219193_v178_n16_p4814_Petroni |
Aporte de: |
id |
paperaa:paper_00219193_v178_n16_p4814_Petroni |
---|---|
record_format |
dspace |
spelling |
paperaa:paper_00219193_v178_n16_p4814_Petroni2023-06-12T16:42:42Z Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum J. BACTERIOL. 1996;178(16):4814-4821 Petroni, E.A. Ielpi, L. acetylsalicylic acid bacterial dna bacterial enzyme polymer polysaccharide transferase xanthan acetobacter article bacterial gene carbohydrate synthesis controlled study gene isolation nonhuman nucleotide sequence open reading frame priority journal xanthomonas Algorithms Amino Acid Sequence Carbohydrate Sequence Cloning, Molecular Conjugation, Genetic Conserved Sequence Escherichia coli Gene Library Genes, Bacterial Genetic Complementation Test Gluconacetobacter xylinus Mannosyltransferases Molecular Sequence Data Mutagenesis Open Reading Frames Polysaccharides, Bacterial Recombinant Proteins Sequence Homology, Amino Acid Xanthomonas Acetobacter Bacteria (microorganisms) Gluconacetobacter xylinus Xanthomonas Xanthomonas campestris A genetic locus from Acetobacter xylinum involved in acetan polysaccharide synthesis has been characterized. The chromosomal region was identified by screening a genomic library of A. xylinum in a Xanthomonas campestris mutant defective in xanthan polysaccharide synthesis. The A. xylinum cosmid clone can functionally complement a xanthan-negative mutant. The polymer produced by the recombinant strain was found to be indistinguishable from xanthan. Insertion mutagenesis and subcloning of the cosmid clone combined with complementation studies allowed the identification of a 2.3-kb fragment of A. xylinum chromosomal DNA. The nucleotide sequence of this fragment was analyzed and found to contain an open reading frame (aceA) of 1,182 bp encoding a protein of 43.2 kDa. Results from biochemical and genetic analyses strongly suggest that the aceA gene encodes the GDP- mannose:cellobiosyl-diphosphopolyprenol α-mannosyltransferase enzyme, which is responsible for the transfer of an α-mannosyl residue from GDP-Man to cellobiosyl-diphosphopolyprenol. A search for similarities with other known mannosyltransferases revealed that all bacterial α-mannosyltransferases have a short COOH-termina1 amino acid sequence in common. Fil:Petroni, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ielpi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00219193_v178_n16_p4814_Petroni |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
language |
Inglés |
orig_language_str_mv |
eng |
topic |
acetylsalicylic acid bacterial dna bacterial enzyme polymer polysaccharide transferase xanthan acetobacter article bacterial gene carbohydrate synthesis controlled study gene isolation nonhuman nucleotide sequence open reading frame priority journal xanthomonas Algorithms Amino Acid Sequence Carbohydrate Sequence Cloning, Molecular Conjugation, Genetic Conserved Sequence Escherichia coli Gene Library Genes, Bacterial Genetic Complementation Test Gluconacetobacter xylinus Mannosyltransferases Molecular Sequence Data Mutagenesis Open Reading Frames Polysaccharides, Bacterial Recombinant Proteins Sequence Homology, Amino Acid Xanthomonas Acetobacter Bacteria (microorganisms) Gluconacetobacter xylinus Xanthomonas Xanthomonas campestris |
spellingShingle |
acetylsalicylic acid bacterial dna bacterial enzyme polymer polysaccharide transferase xanthan acetobacter article bacterial gene carbohydrate synthesis controlled study gene isolation nonhuman nucleotide sequence open reading frame priority journal xanthomonas Algorithms Amino Acid Sequence Carbohydrate Sequence Cloning, Molecular Conjugation, Genetic Conserved Sequence Escherichia coli Gene Library Genes, Bacterial Genetic Complementation Test Gluconacetobacter xylinus Mannosyltransferases Molecular Sequence Data Mutagenesis Open Reading Frames Polysaccharides, Bacterial Recombinant Proteins Sequence Homology, Amino Acid Xanthomonas Acetobacter Bacteria (microorganisms) Gluconacetobacter xylinus Xanthomonas Xanthomonas campestris Petroni, E.A. Ielpi, L. Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum |
topic_facet |
acetylsalicylic acid bacterial dna bacterial enzyme polymer polysaccharide transferase xanthan acetobacter article bacterial gene carbohydrate synthesis controlled study gene isolation nonhuman nucleotide sequence open reading frame priority journal xanthomonas Algorithms Amino Acid Sequence Carbohydrate Sequence Cloning, Molecular Conjugation, Genetic Conserved Sequence Escherichia coli Gene Library Genes, Bacterial Genetic Complementation Test Gluconacetobacter xylinus Mannosyltransferases Molecular Sequence Data Mutagenesis Open Reading Frames Polysaccharides, Bacterial Recombinant Proteins Sequence Homology, Amino Acid Xanthomonas Acetobacter Bacteria (microorganisms) Gluconacetobacter xylinus Xanthomonas Xanthomonas campestris |
description |
A genetic locus from Acetobacter xylinum involved in acetan polysaccharide synthesis has been characterized. The chromosomal region was identified by screening a genomic library of A. xylinum in a Xanthomonas campestris mutant defective in xanthan polysaccharide synthesis. The A. xylinum cosmid clone can functionally complement a xanthan-negative mutant. The polymer produced by the recombinant strain was found to be indistinguishable from xanthan. Insertion mutagenesis and subcloning of the cosmid clone combined with complementation studies allowed the identification of a 2.3-kb fragment of A. xylinum chromosomal DNA. The nucleotide sequence of this fragment was analyzed and found to contain an open reading frame (aceA) of 1,182 bp encoding a protein of 43.2 kDa. Results from biochemical and genetic analyses strongly suggest that the aceA gene encodes the GDP- mannose:cellobiosyl-diphosphopolyprenol α-mannosyltransferase enzyme, which is responsible for the transfer of an α-mannosyl residue from GDP-Man to cellobiosyl-diphosphopolyprenol. A search for similarities with other known mannosyltransferases revealed that all bacterial α-mannosyltransferases have a short COOH-termina1 amino acid sequence in common. |
format |
Artículo Artículo publishedVersion |
author |
Petroni, E.A. Ielpi, L. |
author_facet |
Petroni, E.A. Ielpi, L. |
author_sort |
Petroni, E.A. |
title |
Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum |
title_short |
Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum |
title_full |
Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum |
title_fullStr |
Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum |
title_full_unstemmed |
Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum |
title_sort |
isolation and nucleotide sequence of the gdp-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from acetobacter xylinum |
publishDate |
1996 |
url |
http://hdl.handle.net/20.500.12110/paper_00219193_v178_n16_p4814_Petroni |
work_keys_str_mv |
AT petroniea isolationandnucleotidesequenceofthegdpmannosecellobiosyldiphosphopolyprenolamannosyltransferasegenefromacetobacterxylinum AT ielpil isolationandnucleotidesequenceofthegdpmannosecellobiosyldiphosphopolyprenolamannosyltransferasegenefromacetobacterxylinum |
_version_ |
1769810268895838208 |