Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase
1. 1. The effect of several metals and reagents on the decarboxylation rate of uroporphyrinogen I by using a 16-fold purified preparation of Uroporphyrinogen Decarboxylase from Rhodopseudomonas palustris, was studied. 2. 2. 1 mM Hg2+ and Cu2+ were strong inhibitors, 1 mM Zn2+ and Fe2+ under certain...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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1987
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0020711X_v19_n4_p373_Koopmann |
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paperaa:paper_0020711X_v19_n4_p373_Koopmann2023-06-12T16:42:13Z Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase Int. J. Biochem. 1987;19(4):373-377 Koopmann, G.E. Del C. Batlle, A.M. 4 chloromercuribenzoic acid 5,5' dithiobis(2 nitrobenzoic acid) Carboxy Lyases carboxylyase copper ferrous ion mercury metal p Chloromercuribenzoic Acid porphyrin thiol derivative uroporphyrinogen decarboxylase zinc article biosynthesis metabolism Rhodopseudomonas Carboxy-Lyases Chloromercuribenzoates Copper Dithionitrobenzoic Acid Ferrous Compounds Mercury Metals p-Chloromercuribenzoic Acid Porphyrins Rhodopseudomonas Sulfhydryl Compounds Support, Non-U.S. Gov't Uroporphyrinogen Decarboxylase Zinc 1. 1. The effect of several metals and reagents on the decarboxylation rate of uroporphyrinogen I by using a 16-fold purified preparation of Uroporphyrinogen Decarboxylase from Rhodopseudomonas palustris, was studied. 2. 2. 1 mM Hg2+ and Cu2+ were strong inhibitors, 1 mM Zn2+ and Fe2+ under certain conditions and 1 mM Fe3+ and Cr3+ also inactivated the enzyme, but Pb2+, Cd2+, and Al3+ did not. Metals inhibition was reversed by 1 mM GSH or CvSH. 3. 3. 0.1 mM DTNB and PCMB, 1 mM pyridoxal phosphate and 100 mM chloral hydrate, as well as 1 mM 2-methoxy-5-nitrotropone and 0.2 mM diethylpyrocarbonate inhibited Uroporphyrinogen Decarboxylase; while GSH, CySH, N-ethylmaleimide, sodium thioglycolate, 1,4-dithioerythritol, EDTA and O-phenantroline did not modify activity. 4. 4. Data obtained would indicate that one cysteine, one or two histidine residues and probably a lysine group are required for enzyme activity. © 1987. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1987 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0020711X_v19_n4_p373_Koopmann |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
4 chloromercuribenzoic acid 5,5' dithiobis(2 nitrobenzoic acid) Carboxy Lyases carboxylyase copper ferrous ion mercury metal p Chloromercuribenzoic Acid porphyrin thiol derivative uroporphyrinogen decarboxylase zinc article biosynthesis metabolism Rhodopseudomonas Carboxy-Lyases Chloromercuribenzoates Copper Dithionitrobenzoic Acid Ferrous Compounds Mercury Metals p-Chloromercuribenzoic Acid Porphyrins Rhodopseudomonas Sulfhydryl Compounds Support, Non-U.S. Gov't Uroporphyrinogen Decarboxylase Zinc |
| spellingShingle |
4 chloromercuribenzoic acid 5,5' dithiobis(2 nitrobenzoic acid) Carboxy Lyases carboxylyase copper ferrous ion mercury metal p Chloromercuribenzoic Acid porphyrin thiol derivative uroporphyrinogen decarboxylase zinc article biosynthesis metabolism Rhodopseudomonas Carboxy-Lyases Chloromercuribenzoates Copper Dithionitrobenzoic Acid Ferrous Compounds Mercury Metals p-Chloromercuribenzoic Acid Porphyrins Rhodopseudomonas Sulfhydryl Compounds Support, Non-U.S. Gov't Uroporphyrinogen Decarboxylase Zinc Koopmann, G.E. Del C. Batlle, A.M. Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase |
| topic_facet |
4 chloromercuribenzoic acid 5,5' dithiobis(2 nitrobenzoic acid) Carboxy Lyases carboxylyase copper ferrous ion mercury metal p Chloromercuribenzoic Acid porphyrin thiol derivative uroporphyrinogen decarboxylase zinc article biosynthesis metabolism Rhodopseudomonas Carboxy-Lyases Chloromercuribenzoates Copper Dithionitrobenzoic Acid Ferrous Compounds Mercury Metals p-Chloromercuribenzoic Acid Porphyrins Rhodopseudomonas Sulfhydryl Compounds Support, Non-U.S. Gov't Uroporphyrinogen Decarboxylase Zinc |
| description |
1. 1. The effect of several metals and reagents on the decarboxylation rate of uroporphyrinogen I by using a 16-fold purified preparation of Uroporphyrinogen Decarboxylase from Rhodopseudomonas palustris, was studied. 2. 2. 1 mM Hg2+ and Cu2+ were strong inhibitors, 1 mM Zn2+ and Fe2+ under certain conditions and 1 mM Fe3+ and Cr3+ also inactivated the enzyme, but Pb2+, Cd2+, and Al3+ did not. Metals inhibition was reversed by 1 mM GSH or CvSH. 3. 3. 0.1 mM DTNB and PCMB, 1 mM pyridoxal phosphate and 100 mM chloral hydrate, as well as 1 mM 2-methoxy-5-nitrotropone and 0.2 mM diethylpyrocarbonate inhibited Uroporphyrinogen Decarboxylase; while GSH, CySH, N-ethylmaleimide, sodium thioglycolate, 1,4-dithioerythritol, EDTA and O-phenantroline did not modify activity. 4. 4. Data obtained would indicate that one cysteine, one or two histidine residues and probably a lysine group are required for enzyme activity. © 1987. |
| format |
Artículo Artículo publishedVersion |
| author |
Koopmann, G.E. Del C. Batlle, A.M. |
| author_facet |
Koopmann, G.E. Del C. Batlle, A.M. |
| author_sort |
Koopmann, G.E. |
| title |
Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase |
| title_short |
Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase |
| title_full |
Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase |
| title_fullStr |
Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase |
| title_full_unstemmed |
Biosynthesis of porphyrins in Rhodopseudomonas palustris-VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase |
| title_sort |
biosynthesis of porphyrins in rhodopseudomonas palustris-vi. the effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase |
| publishDate |
1987 |
| url |
http://hdl.handle.net/20.500.12110/paper_0020711X_v19_n4_p373_Koopmann |
| work_keys_str_mv |
AT koopmannge biosynthesisofporphyrinsinrhodopseudomonaspalustrisvitheeffectofmetalsthiolsandotherreagentsontheactivityofuroporphyrinogendecarboxylase AT delcbatlleam biosynthesisofporphyrinsinrhodopseudomonaspalustrisvitheeffectofmetalsthiolsandotherreagentsontheactivityofuroporphyrinogendecarboxylase |
| _version_ |
1769810077676470272 |