Heme synthesis in Crithidia deanei: Influence of the endosymbiote

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1. 1. The activity of the following enzymes involved in the biosynthesis of porphyrins was determined in endosymbiote-free and endosymbiote-containing Crithidia deanei grown in a chemically defined medium: succinyl Coenzyme A synthetase (Suc.CoA-S), 5-aminolevulinate synthetase (ALA-S), 4,5-dioxoval...

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Detalles Bibliográficos
Autores principales: Salzman, T.A., Del Batlle, C.A.M., Angluster, J., De Souza, W.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 1985
Materias:
enzyme
heme
crithidia deanei
nonhuman
protozoon
5-Aminolevulinate Synthetase
Aminolevulinic Acid
Comparative Study
Crithidia
Heme
Porphyrins
Rickettsiaceae
Succinate-CoA Ligases
Support, Non-U.S. Gov't
Symbiosis
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0020711X_v17_n12_p1343_Salzman
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:1. 1. The activity of the following enzymes involved in the biosynthesis of porphyrins was determined in endosymbiote-free and endosymbiote-containing Crithidia deanei grown in a chemically defined medium: succinyl Coenzyme A synthetase (Suc.CoA-S), 5-aminolevulinate synthetase (ALA-S), 4,5-dioxovaleric acid transaminase (DOVA-T), 5-aminolevulinate dehydratase (ALA-D), por- phobilinogenase (PBGase), deaminase and heme synthetase (Heme-S). The amount of 5-aminolevulinic acid (ALA) and porphobilinogen, porphyrins and heme was also determined. 2. 2. ALA and PBG were detected in C. deanei. The levels of free porphyrins was low. Heme concentration was nil. 3. 3. The activity of ALA-D. deaminase and PBGase was not detected in C deanei. 4. 4. The activity of Suc.CoA-S and ALA-S were twice higher in symbiote-containing than in aposymbiotic C. deanei. Aposymbiotic cells had a higher activity of DOVA-T than symbiote-containing cells. 5. 5. The level of Heme-S, measured using protoporphyrin as substrate, was twice as high in symbiotecontaining than in symbiote-free cells,. © 1985.

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