The measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria

1. 1. This paper confirms the increase in sensitivity obtained for erythrocyte UIS measurement by pre-incubation of the red cells in a 0.2% Triton-X 100 solution containing 1 mmol/1 ZnSO4 and dithiothreitol as described by Piepkorn et al. (1978). 2. 2. To achieve optimal precision in this assay a su...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Brocklehurst, D., Wider De Xifra, E.A., Batlle, A.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 1980
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0020711X_v12_n5-6_p791_Brocklehurst
Aporte de:
id paperaa:paper_0020711X_v12_n5-6_p791_Brocklehurst
record_format dspace
spelling paperaa:paper_0020711X_v12_n5-6_p791_Brocklehurst2023-06-12T16:42:09Z The measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria Int. J. Biochem. 1980;12(5-6):791-793 Brocklehurst, D. Wider De Xifra, E.A. Batlle, A. blood and hemopoietic system erythrocyte methodology porphyria uroporphyrinogen synthase Acute Disease Adult Aged Ammonia-Lyases Chronic Disease Comparative Study Enzyme Tests Erythrocytes Female Human Hydroxymethylbilane Synthase Kinetics Male Pedigree Porphyria 1. 1. This paper confirms the increase in sensitivity obtained for erythrocyte UIS measurement by pre-incubation of the red cells in a 0.2% Triton-X 100 solution containing 1 mmol/1 ZnSO4 and dithiothreitol as described by Piepkorn et al. (1978). 2. 2. To achieve optimal precision in this assay a substrate concentration of delta aminolaevulinic acid (ALA) of 1000nmol/ml is required. Interpretation of the results obtained by this method is discussed and its use in the detection of both latent and acute intermittent porphyria is demonstrated. Comparative studies were carried out by using the Batlle et al. (1978) method and a modification employing ALA as substrate. © 1980. Fil:Wider De Xifra, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1980 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0020711X_v12_n5-6_p791_Brocklehurst
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic blood and hemopoietic system
erythrocyte
methodology
porphyria
uroporphyrinogen synthase
Acute Disease
Adult
Aged
Ammonia-Lyases
Chronic Disease
Comparative Study
Enzyme Tests
Erythrocytes
Female
Human
Hydroxymethylbilane Synthase
Kinetics
Male
Pedigree
Porphyria
spellingShingle blood and hemopoietic system
erythrocyte
methodology
porphyria
uroporphyrinogen synthase
Acute Disease
Adult
Aged
Ammonia-Lyases
Chronic Disease
Comparative Study
Enzyme Tests
Erythrocytes
Female
Human
Hydroxymethylbilane Synthase
Kinetics
Male
Pedigree
Porphyria
Brocklehurst, D.
Wider De Xifra, E.A.
Batlle, A.
The measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria
topic_facet blood and hemopoietic system
erythrocyte
methodology
porphyria
uroporphyrinogen synthase
Acute Disease
Adult
Aged
Ammonia-Lyases
Chronic Disease
Comparative Study
Enzyme Tests
Erythrocytes
Female
Human
Hydroxymethylbilane Synthase
Kinetics
Male
Pedigree
Porphyria
description 1. 1. This paper confirms the increase in sensitivity obtained for erythrocyte UIS measurement by pre-incubation of the red cells in a 0.2% Triton-X 100 solution containing 1 mmol/1 ZnSO4 and dithiothreitol as described by Piepkorn et al. (1978). 2. 2. To achieve optimal precision in this assay a substrate concentration of delta aminolaevulinic acid (ALA) of 1000nmol/ml is required. Interpretation of the results obtained by this method is discussed and its use in the detection of both latent and acute intermittent porphyria is demonstrated. Comparative studies were carried out by using the Batlle et al. (1978) method and a modification employing ALA as substrate. © 1980.
format Artículo
Artículo
publishedVersion
author Brocklehurst, D.
Wider De Xifra, E.A.
Batlle, A.
author_facet Brocklehurst, D.
Wider De Xifra, E.A.
Batlle, A.
author_sort Brocklehurst, D.
title The measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria
title_short The measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria
title_full The measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria
title_fullStr The measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria
title_full_unstemmed The measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria
title_sort measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria
publishDate 1980
url http://hdl.handle.net/20.500.12110/paper_0020711X_v12_n5-6_p791_Brocklehurst
work_keys_str_mv AT brocklehurstd themeasurementoferythrocyteuroporphyrinogenisynthaseinthediagnosisoflatentandacuteintermittentporphyria
AT widerdexifraea themeasurementoferythrocyteuroporphyrinogenisynthaseinthediagnosisoflatentandacuteintermittentporphyria
AT batllea themeasurementoferythrocyteuroporphyrinogenisynthaseinthediagnosisoflatentandacuteintermittentporphyria
AT brocklehurstd measurementoferythrocyteuroporphyrinogenisynthaseinthediagnosisoflatentandacuteintermittentporphyria
AT widerdexifraea measurementoferythrocyteuroporphyrinogenisynthaseinthediagnosisoflatentandacuteintermittentporphyria
AT batllea measurementoferythrocyteuroporphyrinogenisynthaseinthediagnosisoflatentandacuteintermittentporphyria
_version_ 1769810372315840512