Brucella lumazine synthase elicits a mixed Th1-Th2 immune response and reduces infection in mice challenged with Brucella abortus 544 independently of the adjuvant formulation used
The immunogenicity and protective efficacy of recombinant lumazine synthase from Brucella spp. (rBLS) administered with different adjuvants was evaluated in mice. Mice were immunized with rBLS in the absence or the presence of aluminum hydroxide gel (BLS-A1), monophosphoryl lipid A (BLS-MPA), or inc...
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| Autores principales: | , , , , , , , |
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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2003
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00199567_v71_n10_p5750_Velikovsky |
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paperaa:paper_00199567_v71_n10_p5750_Velikovsky2023-06-12T16:42:06Z Brucella lumazine synthase elicits a mixed Th1-Th2 immune response and reduces infection in mice challenged with Brucella abortus 544 independently of the adjuvant formulation used Infect. Immun. 2003;71(10):5750-5755 Velikovsky, C.A. Goldbaum, F.A. Cassataro, J. Estein, S. Bowden, R.A. Bruno, L. Fossati, C.A. Giambartolomei, G.H. aluminum hydroxide Brucella vaccine Freund adjuvant gamma interferon immunoglobulin G immunoglobulin G antibody immunoglobulin G1 immunoglobulin G2 interleukin 10 interleukin 2 interleukin 4 lumazine lumazine synthase phosphoryl lipid A recombinant enzyme unclassified drug animal experiment animal model article Brucella abortus brucellosis controlled study cytokine production drug effect drug formulation female immune response immunization immunogenicity mouse nonhuman priority journal spleen cell Th1 cell Th2 cell Adjuvants, Immunologic Animals Antibodies, Bacterial Brucella Brucella Vaccine Brucellosis Female Lymphocyte Activation Mice Mice, Inbred BALB C Multienzyme Complexes Th1 Cells Th2 Cells Vaccines, Synthetic The immunogenicity and protective efficacy of recombinant lumazine synthase from Brucella spp. (rBLS) administered with different adjuvants was evaluated in mice. Mice were immunized with rBLS in the absence or the presence of aluminum hydroxide gel (BLS-A1), monophosphoryl lipid A (BLS-MPA), or incomplete Freund's adjuvant (BLS-IFA). rBLS per se induced a vigorous immunoglobulin G (IgG) response, with high titers of IgG1 as well as IgG2. All the adjuvants increased this response; the BLS-IFA formulation was the most effective at inducing BLS-specific IgG antibodies. In addition, after in vitro stimulation with rBLS, spleen cells from BLS-IFA-, BLS-A1-, or BLS-MPA-immunized mice proliferated and produced interleukin-2 (IL-2), gamma interferon (IFN-γ), IL-10, and IL-4, suggesting the induction of a mixed Th1-Th2 response. Immunization with rBLS protected mice against challenge with B. abortus 544. The levels of protection in the spleen were similar for all adjuvants, but only BLS-A1 and BLS-IFA were effective in the liver. Our results indicate that BLS might be a useful candidate for the development of subunit vaccines against brucellosis, since it elicits antigen-specific cellular responses, with production of IFN-γ and protection, independently of the adjuvant formulation used. 2003 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00199567_v71_n10_p5750_Velikovsky |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
aluminum hydroxide Brucella vaccine Freund adjuvant gamma interferon immunoglobulin G immunoglobulin G antibody immunoglobulin G1 immunoglobulin G2 interleukin 10 interleukin 2 interleukin 4 lumazine lumazine synthase phosphoryl lipid A recombinant enzyme unclassified drug animal experiment animal model article Brucella abortus brucellosis controlled study cytokine production drug effect drug formulation female immune response immunization immunogenicity mouse nonhuman priority journal spleen cell Th1 cell Th2 cell Adjuvants, Immunologic Animals Antibodies, Bacterial Brucella Brucella Vaccine Brucellosis Female Lymphocyte Activation Mice Mice, Inbred BALB C Multienzyme Complexes Th1 Cells Th2 Cells Vaccines, Synthetic |
| spellingShingle |
aluminum hydroxide Brucella vaccine Freund adjuvant gamma interferon immunoglobulin G immunoglobulin G antibody immunoglobulin G1 immunoglobulin G2 interleukin 10 interleukin 2 interleukin 4 lumazine lumazine synthase phosphoryl lipid A recombinant enzyme unclassified drug animal experiment animal model article Brucella abortus brucellosis controlled study cytokine production drug effect drug formulation female immune response immunization immunogenicity mouse nonhuman priority journal spleen cell Th1 cell Th2 cell Adjuvants, Immunologic Animals Antibodies, Bacterial Brucella Brucella Vaccine Brucellosis Female Lymphocyte Activation Mice Mice, Inbred BALB C Multienzyme Complexes Th1 Cells Th2 Cells Vaccines, Synthetic Velikovsky, C.A. Goldbaum, F.A. Cassataro, J. Estein, S. Bowden, R.A. Bruno, L. Fossati, C.A. Giambartolomei, G.H. Brucella lumazine synthase elicits a mixed Th1-Th2 immune response and reduces infection in mice challenged with Brucella abortus 544 independently of the adjuvant formulation used |
| topic_facet |
aluminum hydroxide Brucella vaccine Freund adjuvant gamma interferon immunoglobulin G immunoglobulin G antibody immunoglobulin G1 immunoglobulin G2 interleukin 10 interleukin 2 interleukin 4 lumazine lumazine synthase phosphoryl lipid A recombinant enzyme unclassified drug animal experiment animal model article Brucella abortus brucellosis controlled study cytokine production drug effect drug formulation female immune response immunization immunogenicity mouse nonhuman priority journal spleen cell Th1 cell Th2 cell Adjuvants, Immunologic Animals Antibodies, Bacterial Brucella Brucella Vaccine Brucellosis Female Lymphocyte Activation Mice Mice, Inbred BALB C Multienzyme Complexes Th1 Cells Th2 Cells Vaccines, Synthetic |
| description |
The immunogenicity and protective efficacy of recombinant lumazine synthase from Brucella spp. (rBLS) administered with different adjuvants was evaluated in mice. Mice were immunized with rBLS in the absence or the presence of aluminum hydroxide gel (BLS-A1), monophosphoryl lipid A (BLS-MPA), or incomplete Freund's adjuvant (BLS-IFA). rBLS per se induced a vigorous immunoglobulin G (IgG) response, with high titers of IgG1 as well as IgG2. All the adjuvants increased this response; the BLS-IFA formulation was the most effective at inducing BLS-specific IgG antibodies. In addition, after in vitro stimulation with rBLS, spleen cells from BLS-IFA-, BLS-A1-, or BLS-MPA-immunized mice proliferated and produced interleukin-2 (IL-2), gamma interferon (IFN-γ), IL-10, and IL-4, suggesting the induction of a mixed Th1-Th2 response. Immunization with rBLS protected mice against challenge with B. abortus 544. The levels of protection in the spleen were similar for all adjuvants, but only BLS-A1 and BLS-IFA were effective in the liver. Our results indicate that BLS might be a useful candidate for the development of subunit vaccines against brucellosis, since it elicits antigen-specific cellular responses, with production of IFN-γ and protection, independently of the adjuvant formulation used. |
| format |
Artículo Artículo publishedVersion |
| author |
Velikovsky, C.A. Goldbaum, F.A. Cassataro, J. Estein, S. Bowden, R.A. Bruno, L. Fossati, C.A. Giambartolomei, G.H. |
| author_facet |
Velikovsky, C.A. Goldbaum, F.A. Cassataro, J. Estein, S. Bowden, R.A. Bruno, L. Fossati, C.A. Giambartolomei, G.H. |
| author_sort |
Velikovsky, C.A. |
| title |
Brucella lumazine synthase elicits a mixed Th1-Th2 immune response and reduces infection in mice challenged with Brucella abortus 544 independently of the adjuvant formulation used |
| title_short |
Brucella lumazine synthase elicits a mixed Th1-Th2 immune response and reduces infection in mice challenged with Brucella abortus 544 independently of the adjuvant formulation used |
| title_full |
Brucella lumazine synthase elicits a mixed Th1-Th2 immune response and reduces infection in mice challenged with Brucella abortus 544 independently of the adjuvant formulation used |
| title_fullStr |
Brucella lumazine synthase elicits a mixed Th1-Th2 immune response and reduces infection in mice challenged with Brucella abortus 544 independently of the adjuvant formulation used |
| title_full_unstemmed |
Brucella lumazine synthase elicits a mixed Th1-Th2 immune response and reduces infection in mice challenged with Brucella abortus 544 independently of the adjuvant formulation used |
| title_sort |
brucella lumazine synthase elicits a mixed th1-th2 immune response and reduces infection in mice challenged with brucella abortus 544 independently of the adjuvant formulation used |
| publishDate |
2003 |
| url |
http://hdl.handle.net/20.500.12110/paper_00199567_v71_n10_p5750_Velikovsky |
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