AtTIP1;3 and AtTIP5;1, the only highly expressed Arabidopsis pollen-specific aquaporins, transport water and urea
Pollination includes processes where water and/or solute movements must be finely regulated, suggesting participation of aquaporins. Using information available from different transcriptional profilings of Arabidopsis thaliana mature pollen, we showed that the only aquaporins that are selectively an...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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2008
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00145793_v582_n29_p4077_Soto |
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paperaa:paper_00145793_v582_n29_p4077_Soto2023-06-12T16:41:52Z AtTIP1;3 and AtTIP5;1, the only highly expressed Arabidopsis pollen-specific aquaporins, transport water and urea FEBS Lett. 2008;582(29):4077-4082 Soto, G. Alleva, K. Mazzella, M.A. Amodeo, G. Muschietti, J.P. Arabidopsis Pollen hydration Pollen tube Solute transport Tonoplast aquaporin Urea aquaporin boric acid glycerol urea animal cell Arabidopsis article cell swelling controlled study DNA fingerprinting gene locus genetic transcription nonhuman oocyte pollen priority journal protein expression water permeability water transport Animals Aquaporins Arabidopsis Arabidopsis Proteins Biological Transport Gene Expression Regulation, Plant Pollen Transcription, Genetic Urea Water Xenopus Arabidopsis Arabidopsis thaliana Pollination includes processes where water and/or solute movements must be finely regulated, suggesting participation of aquaporins. Using information available from different transcriptional profilings of Arabidopsis thaliana mature pollen, we showed that the only aquaporins that are selectively and highly expressed in mature pollen are two TIPs: AtTIP1;3 and AtTIP5;1. Pollen exhibited a lower number and more exclusive type of aquaporin expressed genes when compared to other single cell transcriptional profilings. When characterized using Xenopus oocyte swelling assays, AtTIP1;3 and AtTIP5;1 showed intermediate water permeabilities. Although they displayed neither glycerol nor boric acid permeability they both transported urea. In conclusion, these results suggest a function for AtTIP1;3 and AtTIP5;1 as specific water and urea channels in Arabidopsis pollen. © 2008 Federation of European Biochemical Societies. Fil:Soto, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mazzella, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muschietti, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00145793_v582_n29_p4077_Soto |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
Arabidopsis Pollen hydration Pollen tube Solute transport Tonoplast aquaporin Urea aquaporin boric acid glycerol urea animal cell Arabidopsis article cell swelling controlled study DNA fingerprinting gene locus genetic transcription nonhuman oocyte pollen priority journal protein expression water permeability water transport Animals Aquaporins Arabidopsis Arabidopsis Proteins Biological Transport Gene Expression Regulation, Plant Pollen Transcription, Genetic Urea Water Xenopus Arabidopsis Arabidopsis thaliana |
| spellingShingle |
Arabidopsis Pollen hydration Pollen tube Solute transport Tonoplast aquaporin Urea aquaporin boric acid glycerol urea animal cell Arabidopsis article cell swelling controlled study DNA fingerprinting gene locus genetic transcription nonhuman oocyte pollen priority journal protein expression water permeability water transport Animals Aquaporins Arabidopsis Arabidopsis Proteins Biological Transport Gene Expression Regulation, Plant Pollen Transcription, Genetic Urea Water Xenopus Arabidopsis Arabidopsis thaliana Soto, G. Alleva, K. Mazzella, M.A. Amodeo, G. Muschietti, J.P. AtTIP1;3 and AtTIP5;1, the only highly expressed Arabidopsis pollen-specific aquaporins, transport water and urea |
| topic_facet |
Arabidopsis Pollen hydration Pollen tube Solute transport Tonoplast aquaporin Urea aquaporin boric acid glycerol urea animal cell Arabidopsis article cell swelling controlled study DNA fingerprinting gene locus genetic transcription nonhuman oocyte pollen priority journal protein expression water permeability water transport Animals Aquaporins Arabidopsis Arabidopsis Proteins Biological Transport Gene Expression Regulation, Plant Pollen Transcription, Genetic Urea Water Xenopus Arabidopsis Arabidopsis thaliana |
| description |
Pollination includes processes where water and/or solute movements must be finely regulated, suggesting participation of aquaporins. Using information available from different transcriptional profilings of Arabidopsis thaliana mature pollen, we showed that the only aquaporins that are selectively and highly expressed in mature pollen are two TIPs: AtTIP1;3 and AtTIP5;1. Pollen exhibited a lower number and more exclusive type of aquaporin expressed genes when compared to other single cell transcriptional profilings. When characterized using Xenopus oocyte swelling assays, AtTIP1;3 and AtTIP5;1 showed intermediate water permeabilities. Although they displayed neither glycerol nor boric acid permeability they both transported urea. In conclusion, these results suggest a function for AtTIP1;3 and AtTIP5;1 as specific water and urea channels in Arabidopsis pollen. © 2008 Federation of European Biochemical Societies. |
| format |
Artículo Artículo publishedVersion |
| author |
Soto, G. Alleva, K. Mazzella, M.A. Amodeo, G. Muschietti, J.P. |
| author_facet |
Soto, G. Alleva, K. Mazzella, M.A. Amodeo, G. Muschietti, J.P. |
| author_sort |
Soto, G. |
| title |
AtTIP1;3 and AtTIP5;1, the only highly expressed Arabidopsis pollen-specific aquaporins, transport water and urea |
| title_short |
AtTIP1;3 and AtTIP5;1, the only highly expressed Arabidopsis pollen-specific aquaporins, transport water and urea |
| title_full |
AtTIP1;3 and AtTIP5;1, the only highly expressed Arabidopsis pollen-specific aquaporins, transport water and urea |
| title_fullStr |
AtTIP1;3 and AtTIP5;1, the only highly expressed Arabidopsis pollen-specific aquaporins, transport water and urea |
| title_full_unstemmed |
AtTIP1;3 and AtTIP5;1, the only highly expressed Arabidopsis pollen-specific aquaporins, transport water and urea |
| title_sort |
attip1;3 and attip5;1, the only highly expressed arabidopsis pollen-specific aquaporins, transport water and urea |
| publishDate |
2008 |
| url |
http://hdl.handle.net/20.500.12110/paper_00145793_v582_n29_p4077_Soto |
| work_keys_str_mv |
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| _version_ |
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