Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata
DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann (Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene (Adh1) was amplified using exon priming intron crossing-polymerase chain reaction. Three size variants wer...
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Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00138703_v136_n1_p53_Lanzavecchia |
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paperaa:paper_00138703_v136_n1_p53_Lanzavecchia2023-06-12T16:41:41Z Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata Entomol. Exp. Appl. 2010;136(1):53-65 Lanzavecchia, S. Remis, M. Cladera, J. Zandomeni, R. Diptera EPIC-PCR Exon priming intron crossing-polymerase chain reaction Gene flow Medfly Migration patterns Molecular markers Population genetics Tephritidae allele biomarker fly gene flow genetic analysis heterozygosity migration polymerase chain reaction polymorphism Ceratitis capitata Diptera Hexapoda Tephritidae DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann (Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene (Adh1) was amplified using exon priming intron crossing-polymerase chain reaction. Three size variants were detected among the 307 samples analyzed. To better differentiate the size variants, further digestion of PCR products with the EcoRI restriction enzyme was carried out. Complete nucleotide sequences of the three-allele variants were obtained and single changes, insertions, deletions, and EcoRI recognition sites were located. Population allele frequencies were analyzed and a global mean heterozygosity (He) of 0.33 was obtained. In most populations, observed allelic frequencies conformed to Hardy-Weinberg expectations. Significant differences between provinces and sampling sites within these provinces, and among some populations were found. The average number of insects exchanged among populations (Nm) was estimated and high values were observed between Argentina and populations from two African countries (Morocco and Kenya), Australia, and Hawaii (Kauai). Pest introduction sources and dispersion patterns in Argentina are discussed based on these results as well as on available bibliographical data. © 2010 The Authors. Journal compilation © 2010 The Netherlands Entomological Society. Fil:Lanzavecchia, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Remis, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cladera, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00138703_v136_n1_p53_Lanzavecchia |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
language |
Inglés |
orig_language_str_mv |
eng |
topic |
Diptera EPIC-PCR Exon priming intron crossing-polymerase chain reaction Gene flow Medfly Migration patterns Molecular markers Population genetics Tephritidae allele biomarker fly gene flow genetic analysis heterozygosity migration polymerase chain reaction polymorphism Ceratitis capitata Diptera Hexapoda Tephritidae |
spellingShingle |
Diptera EPIC-PCR Exon priming intron crossing-polymerase chain reaction Gene flow Medfly Migration patterns Molecular markers Population genetics Tephritidae allele biomarker fly gene flow genetic analysis heterozygosity migration polymerase chain reaction polymorphism Ceratitis capitata Diptera Hexapoda Tephritidae Lanzavecchia, S. Remis, M. Cladera, J. Zandomeni, R. Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata |
topic_facet |
Diptera EPIC-PCR Exon priming intron crossing-polymerase chain reaction Gene flow Medfly Migration patterns Molecular markers Population genetics Tephritidae allele biomarker fly gene flow genetic analysis heterozygosity migration polymerase chain reaction polymorphism Ceratitis capitata Diptera Hexapoda Tephritidae |
description |
DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann (Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene (Adh1) was amplified using exon priming intron crossing-polymerase chain reaction. Three size variants were detected among the 307 samples analyzed. To better differentiate the size variants, further digestion of PCR products with the EcoRI restriction enzyme was carried out. Complete nucleotide sequences of the three-allele variants were obtained and single changes, insertions, deletions, and EcoRI recognition sites were located. Population allele frequencies were analyzed and a global mean heterozygosity (He) of 0.33 was obtained. In most populations, observed allelic frequencies conformed to Hardy-Weinberg expectations. Significant differences between provinces and sampling sites within these provinces, and among some populations were found. The average number of insects exchanged among populations (Nm) was estimated and high values were observed between Argentina and populations from two African countries (Morocco and Kenya), Australia, and Hawaii (Kauai). Pest introduction sources and dispersion patterns in Argentina are discussed based on these results as well as on available bibliographical data. © 2010 The Authors. Journal compilation © 2010 The Netherlands Entomological Society. |
format |
Artículo Artículo publishedVersion |
author |
Lanzavecchia, S. Remis, M. Cladera, J. Zandomeni, R. |
author_facet |
Lanzavecchia, S. Remis, M. Cladera, J. Zandomeni, R. |
author_sort |
Lanzavecchia, S. |
title |
Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata |
title_short |
Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata |
title_full |
Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata |
title_fullStr |
Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata |
title_full_unstemmed |
Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata |
title_sort |
adh1 first intron polymorphisms in argentinean populations of ceratitis capitata |
publishDate |
2010 |
url |
http://hdl.handle.net/20.500.12110/paper_00138703_v136_n1_p53_Lanzavecchia |
work_keys_str_mv |
AT lanzavecchias adh1firstintronpolymorphismsinargentineanpopulationsofceratitiscapitata AT remism adh1firstintronpolymorphismsinargentineanpopulationsofceratitiscapitata AT claderaj adh1firstintronpolymorphismsinargentineanpopulationsofceratitiscapitata AT zandomenir adh1firstintronpolymorphismsinargentineanpopulationsofceratitiscapitata |
_version_ |
1769810072781717504 |