Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway

Mostrar todas las versiones(3)

Bacterial lipopolysaccharide (LPS) activates the immune system and induces increases in peripheral cytokines, which, in turn, affect the endocrine system. In particular, LPS-induced cytokines stimulate the hypothalamic-pituitary-adrenal axis to increase levels of antiinflammatory-acting glucocortico...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Lohrer, P., Gloddek, J., Carbia Nagashima, A., Korali, Z., Hopfner, U., Paez Pereda, M., Arzt, E., Stalla, G.K., Renner, U.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2000
Materias:
4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole
antibody
CD14 antigen
cycloheximide
dexamethasone
immunoglobulin enhancer binding protein
interleukin 6
lipopolysaccharide
lipopolysaccharide binding protein
mitogen activated protein kinase
toll like receptor 4
animal cell
antigen expression
article
cell line
cell stimulation
complex formation
controlled study
cytokine production
cytokine release
hypophysis cell
male
mouse
nonhuman
priority journal
protein metabolism
protein phosphorylation
signal transduction
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00137227_v141_n12_p4457_Lohrer
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paperaa:paper_00137227_v141_n12_p4457_Lohrer
record_format dspace
spelling paperaa:paper_00137227_v141_n12_p4457_Lohrer2023-06-12T16:41:37Z Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway Endocrinology 2000;141(12):4457-4465 Lohrer, P. Gloddek, J. Carbia Nagashima, A. Korali, Z. Hopfner, U. Paez Pereda, M. Arzt, E. Stalla, G.K. Renner, U. 4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole antibody CD14 antigen cycloheximide dexamethasone immunoglobulin enhancer binding protein interleukin 6 lipopolysaccharide lipopolysaccharide binding protein mitogen activated protein kinase toll like receptor 4 animal cell antigen expression article cell line cell stimulation complex formation controlled study cytokine production cytokine release hypophysis cell male mouse nonhuman priority journal protein metabolism protein phosphorylation signal transduction Bacterial lipopolysaccharide (LPS) activates the immune system and induces increases in peripheral cytokines, which, in turn, affect the endocrine system. In particular, LPS-induced cytokines stimulate the hypothalamic-pituitary-adrenal axis to increase levels of antiinflammatory-acting glucocorticoids. In the present work, we show that LPS directly stimulates interleukin (IL)-6 release by mouse pituitary folliculostellate (FS) TtT/GF tumor cells and FS cells of mouse pituitary cell cultures. The stimulatory effect of LPS was strongly enhanced in the presence of serum, suggesting that LPS is only fully active as a complex with LPS-binding protein (LBP). Both TtT/GF cells and mouse pituitaries expressed CD14, which binds the LPS/LBP complex, and Toll-like receptor type 4, which induces LPS signals. LPS increased phospoinositol turnover in TtT/GF cells and induced phosphorylation of p38α mitogen-activated protein kinase and the inhibitor (IκB) of nuclear factor-κ B. Nuclear factor-κ B was activated by LPS in TtT/GF cells. Functional studies demonstrated that My4 (an antibody blocking the interaction between LPS/LBP and CD14), SB203580, (a specific inhibitor of p38α mitogen-activated protein kinase phosphorylation), dexamethasone, and the messenger RNA translation inhibitor cycloheximide all inhibited LPS-induced IL-6 production by TtT/GF cells and mouse pituitary FS cells. LPS-induced intrapituitary IL-6 may modulate the function of anterior pituitary cells during bacterial infection/inflammation. Fil:Carbia Nagashima, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paez Pereda, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2000 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00137227_v141_n12_p4457_Lohrer
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic 4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole
antibody
CD14 antigen
cycloheximide
dexamethasone
immunoglobulin enhancer binding protein
interleukin 6
lipopolysaccharide
lipopolysaccharide binding protein
mitogen activated protein kinase
toll like receptor 4
animal cell
antigen expression
article
cell line
cell stimulation
complex formation
controlled study
cytokine production
cytokine release
hypophysis cell
male
mouse
nonhuman
priority journal
protein metabolism
protein phosphorylation
signal transduction
spellingShingle 4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole
antibody
CD14 antigen
cycloheximide
dexamethasone
immunoglobulin enhancer binding protein
interleukin 6
lipopolysaccharide
lipopolysaccharide binding protein
mitogen activated protein kinase
toll like receptor 4
animal cell
antigen expression
article
cell line
cell stimulation
complex formation
controlled study
cytokine production
cytokine release
hypophysis cell
male
mouse
nonhuman
priority journal
protein metabolism
protein phosphorylation
signal transduction
Lohrer, P.
Gloddek, J.
Carbia Nagashima, A.
Korali, Z.
Hopfner, U.
Paez Pereda, M.
Arzt, E.
Stalla, G.K.
Renner, U.
Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway
topic_facet 4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole
antibody
CD14 antigen
cycloheximide
dexamethasone
immunoglobulin enhancer binding protein
interleukin 6
lipopolysaccharide
lipopolysaccharide binding protein
mitogen activated protein kinase
toll like receptor 4
animal cell
antigen expression
article
cell line
cell stimulation
complex formation
controlled study
cytokine production
cytokine release
hypophysis cell
male
mouse
nonhuman
priority journal
protein metabolism
protein phosphorylation
signal transduction
description Bacterial lipopolysaccharide (LPS) activates the immune system and induces increases in peripheral cytokines, which, in turn, affect the endocrine system. In particular, LPS-induced cytokines stimulate the hypothalamic-pituitary-adrenal axis to increase levels of antiinflammatory-acting glucocorticoids. In the present work, we show that LPS directly stimulates interleukin (IL)-6 release by mouse pituitary folliculostellate (FS) TtT/GF tumor cells and FS cells of mouse pituitary cell cultures. The stimulatory effect of LPS was strongly enhanced in the presence of serum, suggesting that LPS is only fully active as a complex with LPS-binding protein (LBP). Both TtT/GF cells and mouse pituitaries expressed CD14, which binds the LPS/LBP complex, and Toll-like receptor type 4, which induces LPS signals. LPS increased phospoinositol turnover in TtT/GF cells and induced phosphorylation of p38α mitogen-activated protein kinase and the inhibitor (IκB) of nuclear factor-κ B. Nuclear factor-κ B was activated by LPS in TtT/GF cells. Functional studies demonstrated that My4 (an antibody blocking the interaction between LPS/LBP and CD14), SB203580, (a specific inhibitor of p38α mitogen-activated protein kinase phosphorylation), dexamethasone, and the messenger RNA translation inhibitor cycloheximide all inhibited LPS-induced IL-6 production by TtT/GF cells and mouse pituitary FS cells. LPS-induced intrapituitary IL-6 may modulate the function of anterior pituitary cells during bacterial infection/inflammation.
format Artículo
Artículo
publishedVersion
author Lohrer, P.
Gloddek, J.
Carbia Nagashima, A.
Korali, Z.
Hopfner, U.
Paez Pereda, M.
Arzt, E.
Stalla, G.K.
Renner, U.
author_facet Lohrer, P.
Gloddek, J.
Carbia Nagashima, A.
Korali, Z.
Hopfner, U.
Paez Pereda, M.
Arzt, E.
Stalla, G.K.
Renner, U.
author_sort Lohrer, P.
title Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway
title_short Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway
title_full Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway
title_fullStr Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway
title_full_unstemmed Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway
title_sort lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κb pathway
publishDate 2000
url http://hdl.handle.net/20.500.12110/paper_00137227_v141_n12_p4457_Lohrer
work_keys_str_mv AT lohrerp lipopolysaccharidedirectlystimulatestheintrapituitaryinterleukin6productionbyfolliculostellatecellsviaspecificreceptorsandthep38amitogenactivatedproteinkinasenuclearfactorkbpathway
AT gloddekj lipopolysaccharidedirectlystimulatestheintrapituitaryinterleukin6productionbyfolliculostellatecellsviaspecificreceptorsandthep38amitogenactivatedproteinkinasenuclearfactorkbpathway
AT carbianagashimaa lipopolysaccharidedirectlystimulatestheintrapituitaryinterleukin6productionbyfolliculostellatecellsviaspecificreceptorsandthep38amitogenactivatedproteinkinasenuclearfactorkbpathway
AT koraliz lipopolysaccharidedirectlystimulatestheintrapituitaryinterleukin6productionbyfolliculostellatecellsviaspecificreceptorsandthep38amitogenactivatedproteinkinasenuclearfactorkbpathway
AT hopfneru lipopolysaccharidedirectlystimulatestheintrapituitaryinterleukin6productionbyfolliculostellatecellsviaspecificreceptorsandthep38amitogenactivatedproteinkinasenuclearfactorkbpathway
AT paezperedam lipopolysaccharidedirectlystimulatestheintrapituitaryinterleukin6productionbyfolliculostellatecellsviaspecificreceptorsandthep38amitogenactivatedproteinkinasenuclearfactorkbpathway
AT arzte lipopolysaccharidedirectlystimulatestheintrapituitaryinterleukin6productionbyfolliculostellatecellsviaspecificreceptorsandthep38amitogenactivatedproteinkinasenuclearfactorkbpathway
AT stallagk lipopolysaccharidedirectlystimulatestheintrapituitaryinterleukin6productionbyfolliculostellatecellsviaspecificreceptorsandthep38amitogenactivatedproteinkinasenuclearfactorkbpathway
AT renneru lipopolysaccharidedirectlystimulatestheintrapituitaryinterleukin6productionbyfolliculostellatecellsviaspecificreceptorsandthep38amitogenactivatedproteinkinasenuclearfactorkbpathway
_version_ 1769810312944418816

Ejemplares similares