Evidence for a role of the alternatively spliced ED-I sequence of fibronectin during ovarian follicular development
This study was aimed at testing the hypothesis that different forms of fibronectin (FN), produced as a consequence of the alternative splicing of the precursor messenger RNA, play specific roles during development of the ovarian follicle. In particular, we were interested in determining the effect o...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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1999
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00137227_v140_n6_p2541_ColmanLerner |
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paperaa:paper_00137227_v140_n6_p2541_ColmanLerner2023-06-12T16:41:37Z Evidence for a role of the alternatively spliced ED-I sequence of fibronectin during ovarian follicular development Endocrinology 1999;140(6):2541-2548 Colman-Lerner, A. Fischman, M.L. Lanuza, G.M. Bissell, D.M. Kornblihtt, A.R. Barañao, J.L. cyclic AMP estradiol fibronectin messenger RNA recombinant protein RNA precursor transforming growth factor beta alternative RNA splicing animal cell animal tissue article cattle cell proliferation DNA synthesis female gene expression regulation gene function gene sequence granulosa cell growth regulation mitogenesis nonhuman Northern blotting ovary follicle development ovary follicle fluid priority journal protein content protein domain protein synthesis Western blotting This study was aimed at testing the hypothesis that different forms of fibronectin (FN), produced as a consequence of the alternative splicing of the precursor messenger RNA, play specific roles during development of the ovarian follicle. In particular, we were interested in determining the effect of the ED-I (also termed ED-A) type III repeat, which is absent in the plasma form. Analysis of FN levels in follicular fluids corresponding to different stages of development of bovine follicles revealed marked changes in the concentrations of ED-I + FN, whereas total FN levels remained relatively constant. ED-I + FN levels were higher in small follicles, corresponding to the phase of granulosa cell proliferation. The hypothesis of a physiological role for ED-I + FN was further supported by the finding of a regulation of the alternative splicing of FN in primary cultures of bovine granulosa cells by factors known to control ovarian follicular development. cAMP produced a 10-fold decrease in the relative proportion of the ED-I region. In contrast, transforming growth factor-β elicited a 2-fold stimulation of overall FN synthesis and a 4-fold increase in the synthesis of ED-I containing FN. This effect was evident at the protein (Western blots) and messenger RNA (Northern blots) levels. Although a negative correlation (P < 0.001) was detected between ED-I + FN and estradiol levels in follicular fluid, this steroid was unable to modulate in vitro the alternative splicing of FN. A possible mitogenic effect of ED-I + FN was suggested by the observation that a recombinant peptide corresponding to the ED-I domain stimulated DNA synthesis in a bovine granulosa cell line (BGC-1), whereas a peptide corresponding to the flanking type III sequences had no effect. The hypothesis of ED-I + FN as a growth regulatory factor was further strengthened by the fact that depletion of FN from BGC-1-conditioned medium, which contained ED-I + FN, abrogated its mitogenic activity, whereas plasma FN was without effect. We propose that changes in the primary structure of FN may mediate some of the effects of gonadotropin and intraovarian factors during follicular development. Fil:Colman-Lerner, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lanuza, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, J.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1999 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00137227_v140_n6_p2541_ColmanLerner |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
cyclic AMP estradiol fibronectin messenger RNA recombinant protein RNA precursor transforming growth factor beta alternative RNA splicing animal cell animal tissue article cattle cell proliferation DNA synthesis female gene expression regulation gene function gene sequence granulosa cell growth regulation mitogenesis nonhuman Northern blotting ovary follicle development ovary follicle fluid priority journal protein content protein domain protein synthesis Western blotting |
| spellingShingle |
cyclic AMP estradiol fibronectin messenger RNA recombinant protein RNA precursor transforming growth factor beta alternative RNA splicing animal cell animal tissue article cattle cell proliferation DNA synthesis female gene expression regulation gene function gene sequence granulosa cell growth regulation mitogenesis nonhuman Northern blotting ovary follicle development ovary follicle fluid priority journal protein content protein domain protein synthesis Western blotting Colman-Lerner, A. Fischman, M.L. Lanuza, G.M. Bissell, D.M. Kornblihtt, A.R. Barañao, J.L. Evidence for a role of the alternatively spliced ED-I sequence of fibronectin during ovarian follicular development |
| topic_facet |
cyclic AMP estradiol fibronectin messenger RNA recombinant protein RNA precursor transforming growth factor beta alternative RNA splicing animal cell animal tissue article cattle cell proliferation DNA synthesis female gene expression regulation gene function gene sequence granulosa cell growth regulation mitogenesis nonhuman Northern blotting ovary follicle development ovary follicle fluid priority journal protein content protein domain protein synthesis Western blotting |
| description |
This study was aimed at testing the hypothesis that different forms of fibronectin (FN), produced as a consequence of the alternative splicing of the precursor messenger RNA, play specific roles during development of the ovarian follicle. In particular, we were interested in determining the effect of the ED-I (also termed ED-A) type III repeat, which is absent in the plasma form. Analysis of FN levels in follicular fluids corresponding to different stages of development of bovine follicles revealed marked changes in the concentrations of ED-I + FN, whereas total FN levels remained relatively constant. ED-I + FN levels were higher in small follicles, corresponding to the phase of granulosa cell proliferation. The hypothesis of a physiological role for ED-I + FN was further supported by the finding of a regulation of the alternative splicing of FN in primary cultures of bovine granulosa cells by factors known to control ovarian follicular development. cAMP produced a 10-fold decrease in the relative proportion of the ED-I region. In contrast, transforming growth factor-β elicited a 2-fold stimulation of overall FN synthesis and a 4-fold increase in the synthesis of ED-I containing FN. This effect was evident at the protein (Western blots) and messenger RNA (Northern blots) levels. Although a negative correlation (P < 0.001) was detected between ED-I + FN and estradiol levels in follicular fluid, this steroid was unable to modulate in vitro the alternative splicing of FN. A possible mitogenic effect of ED-I + FN was suggested by the observation that a recombinant peptide corresponding to the ED-I domain stimulated DNA synthesis in a bovine granulosa cell line (BGC-1), whereas a peptide corresponding to the flanking type III sequences had no effect. The hypothesis of ED-I + FN as a growth regulatory factor was further strengthened by the fact that depletion of FN from BGC-1-conditioned medium, which contained ED-I + FN, abrogated its mitogenic activity, whereas plasma FN was without effect. We propose that changes in the primary structure of FN may mediate some of the effects of gonadotropin and intraovarian factors during follicular development. |
| format |
Artículo Artículo publishedVersion |
| author |
Colman-Lerner, A. Fischman, M.L. Lanuza, G.M. Bissell, D.M. Kornblihtt, A.R. Barañao, J.L. |
| author_facet |
Colman-Lerner, A. Fischman, M.L. Lanuza, G.M. Bissell, D.M. Kornblihtt, A.R. Barañao, J.L. |
| author_sort |
Colman-Lerner, A. |
| title |
Evidence for a role of the alternatively spliced ED-I sequence of fibronectin during ovarian follicular development |
| title_short |
Evidence for a role of the alternatively spliced ED-I sequence of fibronectin during ovarian follicular development |
| title_full |
Evidence for a role of the alternatively spliced ED-I sequence of fibronectin during ovarian follicular development |
| title_fullStr |
Evidence for a role of the alternatively spliced ED-I sequence of fibronectin during ovarian follicular development |
| title_full_unstemmed |
Evidence for a role of the alternatively spliced ED-I sequence of fibronectin during ovarian follicular development |
| title_sort |
evidence for a role of the alternatively spliced ed-i sequence of fibronectin during ovarian follicular development |
| publishDate |
1999 |
| url |
http://hdl.handle.net/20.500.12110/paper_00137227_v140_n6_p2541_ColmanLerner |
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