Vasoactive intestinal peptide/vasoactive intestinal peptide receptor relative expression in salivary glands as one endogenous modulator of acinar cell apoptosis in a murine model of Sjögren's syndrome
Sjögren's syndrome (SS) is a chronic autoimmune disease characterized by a progressive oral and ocular dryness that correlates poorly with the autoimmune damage of the glands. It has been proposed that a loss of homeostatic equilibrium in the glands is partly responsible for salivary dysfunctio...
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| Autores principales: | , , , , , , |
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
| Publicado: |
2011
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00099104_v166_n3_p309_Hauk |
| Aporte de: |
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paperaa:paper_00099104_v166_n3_p309_Hauk |
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| record_format |
dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
Acinar cell apoptosis and clearance Sjögren's syndrome VIP cyclic AMP cyclic AMP dependent protein kinase tumor necrosis factor alpha vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 acinar cell animal cell animal experiment animal model animal tissue apoptosis article cell function cell isolation cell survival controlled study female homeostasis inflammation macrophage mouse nonhuman phagocytosis phenotype priority journal protein expression salivary gland Sjoegren syndrome submandibular gland Acinar Cells Animals Apoptosis Autoimmune Diseases Cell Survival Cells, Cultured Cyclic AMP Cyclic AMP-Dependent Protein Kinases Disease Models, Animal Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD NF-kappa B Phagocytosis Receptors, Vasoactive Intestinal Peptide Sjogren's Syndrome Submandibular Gland Tumor Necrosis Factor-alpha Vasoactive Intestinal Peptide |
| spellingShingle |
Acinar cell apoptosis and clearance Sjögren's syndrome VIP cyclic AMP cyclic AMP dependent protein kinase tumor necrosis factor alpha vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 acinar cell animal cell animal experiment animal model animal tissue apoptosis article cell function cell isolation cell survival controlled study female homeostasis inflammation macrophage mouse nonhuman phagocytosis phenotype priority journal protein expression salivary gland Sjoegren syndrome submandibular gland Acinar Cells Animals Apoptosis Autoimmune Diseases Cell Survival Cells, Cultured Cyclic AMP Cyclic AMP-Dependent Protein Kinases Disease Models, Animal Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD NF-kappa B Phagocytosis Receptors, Vasoactive Intestinal Peptide Sjogren's Syndrome Submandibular Gland Tumor Necrosis Factor-alpha Vasoactive Intestinal Peptide Hauk, V. Calafat, M. Larocca, L. Fraccaroli, L. Grasso, E. Ramhorst, R. Leirós, C.P. Vasoactive intestinal peptide/vasoactive intestinal peptide receptor relative expression in salivary glands as one endogenous modulator of acinar cell apoptosis in a murine model of Sjögren's syndrome |
| topic_facet |
Acinar cell apoptosis and clearance Sjögren's syndrome VIP cyclic AMP cyclic AMP dependent protein kinase tumor necrosis factor alpha vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 acinar cell animal cell animal experiment animal model animal tissue apoptosis article cell function cell isolation cell survival controlled study female homeostasis inflammation macrophage mouse nonhuman phagocytosis phenotype priority journal protein expression salivary gland Sjoegren syndrome submandibular gland Acinar Cells Animals Apoptosis Autoimmune Diseases Cell Survival Cells, Cultured Cyclic AMP Cyclic AMP-Dependent Protein Kinases Disease Models, Animal Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD NF-kappa B Phagocytosis Receptors, Vasoactive Intestinal Peptide Sjogren's Syndrome Submandibular Gland Tumor Necrosis Factor-alpha Vasoactive Intestinal Peptide |
| description |
Sjögren's syndrome (SS) is a chronic autoimmune disease characterized by a progressive oral and ocular dryness that correlates poorly with the autoimmune damage of the glands. It has been proposed that a loss of homeostatic equilibrium in the glands is partly responsible for salivary dysfunction with acinar cells involved actively in the pathogenesis of SS. The non-obese diabetic (NOD) mouse model of Sjögren's syndrome develops secretory dysfunction and early loss of glandular homeostatic mechanisms, with mild infiltration of the glands. Based on the vasodilator, prosecretory and trophic effects of the vasoactive intestinal peptide (VIP) on acini as well as its anti-inflammatory properties we hypothesized that the local expression of VIP/vasoactive intestinal peptide receptor (VPAC) system in salivary glands could have a role in acinar cell apoptosis and macrophage function thus influencing gland homeostasis. Here we show a progressive decline of VIP expression in submandibular glands of NOD mice with no changes in VPAC receptor expression compared with normal mice. The deep loss of endogenous VIP was associated with a loss of acinar cells through apoptotic mechanisms that could be induced further by tumour necrosis factor (TNF)-α and reversed by VIP through a cyclic adenosine-5'-monophosphate (cAMP)/protein kinase A (PKA)-mediated pathway. The clearance of apoptotic acinar cells by macrophages was impaired for NOD macrophages but a shift from inflammatory to regulatory phenotype was induced in macrophages during phagocytosis of apoptotic acinar cells. These results support that the decline in endogenous VIP/VPAC local levels might influence the survival/apoptosis intracellular set point in NOD acinar cells and their clearance, thus contributing to gland homeostasis loss. © 2011 The Authors. Clinical and Experimental Immunology © 2011 British Society for Immunology. |
| format |
Artículo Artículo publishedVersion |
| author |
Hauk, V. Calafat, M. Larocca, L. Fraccaroli, L. Grasso, E. Ramhorst, R. Leirós, C.P. |
| author_facet |
Hauk, V. Calafat, M. Larocca, L. Fraccaroli, L. Grasso, E. Ramhorst, R. Leirós, C.P. |
| author_sort |
Hauk, V. |
| title |
Vasoactive intestinal peptide/vasoactive intestinal peptide receptor relative expression in salivary glands as one endogenous modulator of acinar cell apoptosis in a murine model of Sjögren's syndrome |
| title_short |
Vasoactive intestinal peptide/vasoactive intestinal peptide receptor relative expression in salivary glands as one endogenous modulator of acinar cell apoptosis in a murine model of Sjögren's syndrome |
| title_full |
Vasoactive intestinal peptide/vasoactive intestinal peptide receptor relative expression in salivary glands as one endogenous modulator of acinar cell apoptosis in a murine model of Sjögren's syndrome |
| title_fullStr |
Vasoactive intestinal peptide/vasoactive intestinal peptide receptor relative expression in salivary glands as one endogenous modulator of acinar cell apoptosis in a murine model of Sjögren's syndrome |
| title_full_unstemmed |
Vasoactive intestinal peptide/vasoactive intestinal peptide receptor relative expression in salivary glands as one endogenous modulator of acinar cell apoptosis in a murine model of Sjögren's syndrome |
| title_sort |
vasoactive intestinal peptide/vasoactive intestinal peptide receptor relative expression in salivary glands as one endogenous modulator of acinar cell apoptosis in a murine model of sjögren's syndrome |
| publishDate |
2011 |
| url |
http://hdl.handle.net/20.500.12110/paper_00099104_v166_n3_p309_Hauk |
| work_keys_str_mv |
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| _version_ |
1769810069889744896 |
| spelling |
paperaa:paper_00099104_v166_n3_p309_Hauk2023-06-12T16:41:24Z Vasoactive intestinal peptide/vasoactive intestinal peptide receptor relative expression in salivary glands as one endogenous modulator of acinar cell apoptosis in a murine model of Sjögren's syndrome Clin. Exp. Immunol. 2011;166(3):309-316 Hauk, V. Calafat, M. Larocca, L. Fraccaroli, L. Grasso, E. Ramhorst, R. Leirós, C.P. Acinar cell apoptosis and clearance Sjögren's syndrome VIP cyclic AMP cyclic AMP dependent protein kinase tumor necrosis factor alpha vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 acinar cell animal cell animal experiment animal model animal tissue apoptosis article cell function cell isolation cell survival controlled study female homeostasis inflammation macrophage mouse nonhuman phagocytosis phenotype priority journal protein expression salivary gland Sjoegren syndrome submandibular gland Acinar Cells Animals Apoptosis Autoimmune Diseases Cell Survival Cells, Cultured Cyclic AMP Cyclic AMP-Dependent Protein Kinases Disease Models, Animal Macrophages Mice Mice, Inbred BALB C Mice, Inbred NOD NF-kappa B Phagocytosis Receptors, Vasoactive Intestinal Peptide Sjogren's Syndrome Submandibular Gland Tumor Necrosis Factor-alpha Vasoactive Intestinal Peptide Sjögren's syndrome (SS) is a chronic autoimmune disease characterized by a progressive oral and ocular dryness that correlates poorly with the autoimmune damage of the glands. It has been proposed that a loss of homeostatic equilibrium in the glands is partly responsible for salivary dysfunction with acinar cells involved actively in the pathogenesis of SS. The non-obese diabetic (NOD) mouse model of Sjögren's syndrome develops secretory dysfunction and early loss of glandular homeostatic mechanisms, with mild infiltration of the glands. Based on the vasodilator, prosecretory and trophic effects of the vasoactive intestinal peptide (VIP) on acini as well as its anti-inflammatory properties we hypothesized that the local expression of VIP/vasoactive intestinal peptide receptor (VPAC) system in salivary glands could have a role in acinar cell apoptosis and macrophage function thus influencing gland homeostasis. Here we show a progressive decline of VIP expression in submandibular glands of NOD mice with no changes in VPAC receptor expression compared with normal mice. The deep loss of endogenous VIP was associated with a loss of acinar cells through apoptotic mechanisms that could be induced further by tumour necrosis factor (TNF)-α and reversed by VIP through a cyclic adenosine-5'-monophosphate (cAMP)/protein kinase A (PKA)-mediated pathway. The clearance of apoptotic acinar cells by macrophages was impaired for NOD macrophages but a shift from inflammatory to regulatory phenotype was induced in macrophages during phagocytosis of apoptotic acinar cells. These results support that the decline in endogenous VIP/VPAC local levels might influence the survival/apoptosis intracellular set point in NOD acinar cells and their clearance, thus contributing to gland homeostasis loss. © 2011 The Authors. Clinical and Experimental Immunology © 2011 British Society for Immunology. Fil:Hauk, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calafat, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Larocca, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fraccaroli, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grasso, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramhorst, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Leirós, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00099104_v166_n3_p309_Hauk |