Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer
The role of oxidative stress in prostate cancer has been increasingly recognised. Acute and chronic inflammations generate reactive oxygen species that result in damage to cellular structures. Haeme oxygenase-1 (HO-1) has cytoprotective effects against oxidative damage. We hypothesise that modulatio...
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2007
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paperaa:paper_00070920_v97_n12_p1683_Sacca2023-06-12T16:41:22Z Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer Br. J. Cancer 2007;97(12):1683-1689 Sacca, P. Meiss, R. Casas, G. Mazza, O. Calvo, J.C. Navone, N. Vazquez, E. Haeme oxygenase-1 Nuclear localisation Oxidative stress Prostate cancer heme oxygenase 1 hemin adult aged article cancer cell culture cancer growth carcinogenesis cell compartmentalization cell nucleus cell protection controlled study disease association disease course enzyme localization enzyme regulation Gleason score human human cell human tissue immunohistochemistry male oxidative stress priority journal prostate cancer prostate hypertrophy prostatectomy protein expression Western blotting Active Transport, Cell Nucleus Adult Aged Aged, 80 and over Cell Nucleus Heme Oxygenase-1 Hemin Humans Male Middle Aged Prostatic Hyperplasia Prostatic Neoplasms Tumor Cells, Cultured The role of oxidative stress in prostate cancer has been increasingly recognised. Acute and chronic inflammations generate reactive oxygen species that result in damage to cellular structures. Haeme oxygenase-1 (HO-1) has cytoprotective effects against oxidative damage. We hypothesise that modulation of HO-1 expression may be involved in the process of prostate carcinogenesis and prostate cancer progression. We thus studied HO-1 expression and localisation in 85 samples of organ-confined primary prostate cancer obtained via radical prostatectomy (Gleason grades 4-9) and in 39 specimens of benign prostatic hyperplasia (BPH). We assessed HO-1 expression by immunohistochemical staining. No significant difference was observed in the cytoplasmic positive reactivity among tumours (84%), non-neoplastic surrounding parenchyma (89%), or BPH samples (87%) (P=0.53). Haeme oxygenase-1 immunostaining was detected in the nuclei of prostate cancer cells in 55 of 85 (65%) patients but less often in non-neoplastic surrounding parenchyma (30 of 85, 35%) or in BPH (9 of 39, 23%) (P<0.0001). Immunocytochemical and western blot analysis showed HO-1 only in the cytoplasmic compartment of PC3 and LNCaP prostate cancer cell lines. Treatment with hemin, a well-known specific inducer of HO-1, led to clear nuclear localisation of HO-1 in both cell lines and highly induced HO-1 expression in both cellular compartments. These findings have demonstrated, for the first time, that HO-1 expression and nuclear localisation can define a new subgroup of prostate cancer primary tumours and that the modulation of HO-1 expression and its nuclear translocation could represent new avenues for therapy. © 2007 Cancer Research UK. Fil:Sacca, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vazquez, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00070920_v97_n12_p1683_Sacca |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
language |
Inglés |
orig_language_str_mv |
eng |
topic |
Haeme oxygenase-1 Nuclear localisation Oxidative stress Prostate cancer heme oxygenase 1 hemin adult aged article cancer cell culture cancer growth carcinogenesis cell compartmentalization cell nucleus cell protection controlled study disease association disease course enzyme localization enzyme regulation Gleason score human human cell human tissue immunohistochemistry male oxidative stress priority journal prostate cancer prostate hypertrophy prostatectomy protein expression Western blotting Active Transport, Cell Nucleus Adult Aged Aged, 80 and over Cell Nucleus Heme Oxygenase-1 Hemin Humans Male Middle Aged Prostatic Hyperplasia Prostatic Neoplasms Tumor Cells, Cultured |
spellingShingle |
Haeme oxygenase-1 Nuclear localisation Oxidative stress Prostate cancer heme oxygenase 1 hemin adult aged article cancer cell culture cancer growth carcinogenesis cell compartmentalization cell nucleus cell protection controlled study disease association disease course enzyme localization enzyme regulation Gleason score human human cell human tissue immunohistochemistry male oxidative stress priority journal prostate cancer prostate hypertrophy prostatectomy protein expression Western blotting Active Transport, Cell Nucleus Adult Aged Aged, 80 and over Cell Nucleus Heme Oxygenase-1 Hemin Humans Male Middle Aged Prostatic Hyperplasia Prostatic Neoplasms Tumor Cells, Cultured Sacca, P. Meiss, R. Casas, G. Mazza, O. Calvo, J.C. Navone, N. Vazquez, E. Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer |
topic_facet |
Haeme oxygenase-1 Nuclear localisation Oxidative stress Prostate cancer heme oxygenase 1 hemin adult aged article cancer cell culture cancer growth carcinogenesis cell compartmentalization cell nucleus cell protection controlled study disease association disease course enzyme localization enzyme regulation Gleason score human human cell human tissue immunohistochemistry male oxidative stress priority journal prostate cancer prostate hypertrophy prostatectomy protein expression Western blotting Active Transport, Cell Nucleus Adult Aged Aged, 80 and over Cell Nucleus Heme Oxygenase-1 Hemin Humans Male Middle Aged Prostatic Hyperplasia Prostatic Neoplasms Tumor Cells, Cultured |
description |
The role of oxidative stress in prostate cancer has been increasingly recognised. Acute and chronic inflammations generate reactive oxygen species that result in damage to cellular structures. Haeme oxygenase-1 (HO-1) has cytoprotective effects against oxidative damage. We hypothesise that modulation of HO-1 expression may be involved in the process of prostate carcinogenesis and prostate cancer progression. We thus studied HO-1 expression and localisation in 85 samples of organ-confined primary prostate cancer obtained via radical prostatectomy (Gleason grades 4-9) and in 39 specimens of benign prostatic hyperplasia (BPH). We assessed HO-1 expression by immunohistochemical staining. No significant difference was observed in the cytoplasmic positive reactivity among tumours (84%), non-neoplastic surrounding parenchyma (89%), or BPH samples (87%) (P=0.53). Haeme oxygenase-1 immunostaining was detected in the nuclei of prostate cancer cells in 55 of 85 (65%) patients but less often in non-neoplastic surrounding parenchyma (30 of 85, 35%) or in BPH (9 of 39, 23%) (P<0.0001). Immunocytochemical and western blot analysis showed HO-1 only in the cytoplasmic compartment of PC3 and LNCaP prostate cancer cell lines. Treatment with hemin, a well-known specific inducer of HO-1, led to clear nuclear localisation of HO-1 in both cell lines and highly induced HO-1 expression in both cellular compartments. These findings have demonstrated, for the first time, that HO-1 expression and nuclear localisation can define a new subgroup of prostate cancer primary tumours and that the modulation of HO-1 expression and its nuclear translocation could represent new avenues for therapy. © 2007 Cancer Research UK. |
format |
Artículo Artículo publishedVersion |
author |
Sacca, P. Meiss, R. Casas, G. Mazza, O. Calvo, J.C. Navone, N. Vazquez, E. |
author_facet |
Sacca, P. Meiss, R. Casas, G. Mazza, O. Calvo, J.C. Navone, N. Vazquez, E. |
author_sort |
Sacca, P. |
title |
Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer |
title_short |
Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer |
title_full |
Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer |
title_fullStr |
Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer |
title_full_unstemmed |
Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer |
title_sort |
nuclear translocation of haeme oxygenase-1 is associated to prostate cancer |
publishDate |
2007 |
url |
http://hdl.handle.net/20.500.12110/paper_00070920_v97_n12_p1683_Sacca |
work_keys_str_mv |
AT saccap nucleartranslocationofhaemeoxygenase1isassociatedtoprostatecancer AT meissr nucleartranslocationofhaemeoxygenase1isassociatedtoprostatecancer AT casasg nucleartranslocationofhaemeoxygenase1isassociatedtoprostatecancer AT mazzao nucleartranslocationofhaemeoxygenase1isassociatedtoprostatecancer AT calvojc nucleartranslocationofhaemeoxygenase1isassociatedtoprostatecancer AT navonen nucleartranslocationofhaemeoxygenase1isassociatedtoprostatecancer AT vazqueze nucleartranslocationofhaemeoxygenase1isassociatedtoprostatecancer |
_version_ |
1769810155157848064 |