17Beta-estradiol enhances leptin expression in human placental cells through genomic and nongenomic actions
The process of embryo implantation and trophoblast invasion is considered the most limiting factor in the establishment of pregnancy. Leptin was originally described as an adipocyte-derived signaling molecule for the central control of metabolism. However, it has been suggested that leptin is involv...
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| Autores principales: | , , , , , , |
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
| Publicado: |
2010
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00063363_v83_n1_p42_Gambino |
| Aporte de: |
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paperaa:paper_00063363_v83_n1_p42_Gambino |
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| record_format |
dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
17beta-estradiol Gene expression Leptin MAPK signal transduction pathway Placenta 2 (2 amino 3 methoxyphenyl)chromone estradiol estrogen receptor estrogen receptor alpha fulvestrant leptin luciferase mitogen activated protein kinase phosphatidylinositol 3 kinase wortmannin estradiol estrogen receptor alpha estrogen receptor alpha, human fulvestrant leptin mitogen activated protein kinase phosphatidylinositol 3 kinase protein kinase B adipocyte article cancer cell culture choriocarcinoma concentration response controlled study embryo enzyme inhibition enzyme phosphorylation explant gene deletion gene expression human human cell human tissue nidation placenta plasmid pregnancy priority journal promoter region reporter gene signal transduction transient transfection trophoblast Western blotting analogs and derivatives antagonists and inhibitors female in vitro study metabolism placenta signal transduction tumor cell line Bovinae Cell Line, Tumor Estradiol Estrogen Receptor alpha Extracellular Signal-Regulated MAP Kinases Female Humans In Vitro Techniques Leptin MAP Kinase Signaling System Phosphatidylinositol 3-Kinases Placenta Pregnancy Promoter Regions, Genetic Proto-Oncogene Proteins c-akt Receptor Cross-Talk |
| spellingShingle |
17beta-estradiol Gene expression Leptin MAPK signal transduction pathway Placenta 2 (2 amino 3 methoxyphenyl)chromone estradiol estrogen receptor estrogen receptor alpha fulvestrant leptin luciferase mitogen activated protein kinase phosphatidylinositol 3 kinase wortmannin estradiol estrogen receptor alpha estrogen receptor alpha, human fulvestrant leptin mitogen activated protein kinase phosphatidylinositol 3 kinase protein kinase B adipocyte article cancer cell culture choriocarcinoma concentration response controlled study embryo enzyme inhibition enzyme phosphorylation explant gene deletion gene expression human human cell human tissue nidation placenta plasmid pregnancy priority journal promoter region reporter gene signal transduction transient transfection trophoblast Western blotting analogs and derivatives antagonists and inhibitors female in vitro study metabolism placenta signal transduction tumor cell line Bovinae Cell Line, Tumor Estradiol Estrogen Receptor alpha Extracellular Signal-Regulated MAP Kinases Female Humans In Vitro Techniques Leptin MAP Kinase Signaling System Phosphatidylinositol 3-Kinases Placenta Pregnancy Promoter Regions, Genetic Proto-Oncogene Proteins c-akt Receptor Cross-Talk Gambino, Y.P. Maymó, J.L. Pérez-Pérez, A. Dueñas, J.L. Sánchez-Margalet, V. Calvo, J.C. Varone, C.L. 17Beta-estradiol enhances leptin expression in human placental cells through genomic and nongenomic actions |
| topic_facet |
17beta-estradiol Gene expression Leptin MAPK signal transduction pathway Placenta 2 (2 amino 3 methoxyphenyl)chromone estradiol estrogen receptor estrogen receptor alpha fulvestrant leptin luciferase mitogen activated protein kinase phosphatidylinositol 3 kinase wortmannin estradiol estrogen receptor alpha estrogen receptor alpha, human fulvestrant leptin mitogen activated protein kinase phosphatidylinositol 3 kinase protein kinase B adipocyte article cancer cell culture choriocarcinoma concentration response controlled study embryo enzyme inhibition enzyme phosphorylation explant gene deletion gene expression human human cell human tissue nidation placenta plasmid pregnancy priority journal promoter region reporter gene signal transduction transient transfection trophoblast Western blotting analogs and derivatives antagonists and inhibitors female in vitro study metabolism placenta signal transduction tumor cell line Bovinae Cell Line, Tumor Estradiol Estrogen Receptor alpha Extracellular Signal-Regulated MAP Kinases Female Humans In Vitro Techniques Leptin MAP Kinase Signaling System Phosphatidylinositol 3-Kinases Placenta Pregnancy Promoter Regions, Genetic Proto-Oncogene Proteins c-akt Receptor Cross-Talk |
| description |
The process of embryo implantation and trophoblast invasion is considered the most limiting factor in the establishment of pregnancy. Leptin was originally described as an adipocyte-derived signaling molecule for the central control of metabolism. However, it has been suggested that leptin is involved in other functions during pregnancy, particularly in the placenta, where it was found to be expressed. In the present work, we have found a stimulatory effect of 17beta-estradiol (E2) on endogenous leptin expression, as analyzed by Western blot, in both the BeWo choriocarcinoma cell line and normal placental explants. This effect was time and dose dependent. Maximal effect was achieved at 10 nM in BeWo cells and 1 nM in placental explants. The E 2 effects involved the estrogen receptor, as the antagonist ICI 182 780 inhibited E2-induced leptin expression. Moreover, E2 treatment enhanced leptin promoter activity up to 4-fold, as evaluated by transient transfection with a plasmid construction containing the leptin promoter region and the reporter gene luciferase. This effect was dose dependent. Deletion analysis demonstrated that a minimal promoter region between - 1951 and -1847 bp is both necessary and sufficient to achieve E2 effects. Estradiol action involved estrogen receptor 1, previously known as estrogen receptor alpha, as cotransfection with a vector encoding estrogen receptor 1 potentiated the effects of E2 on leptin expression. Moreover, E2 action probably involves membrane receptors too, as treatment with an estradiol-bovine serum albumin complex partially enhanced leptin expression. The effects of E2 could be blocked by pharmacologic inhibition of MAPK and the phosphoinositide-3-kinase (PI3K) pathways with 50 μM PD98059 and 0.1 μM Wortmannin, respectively. Moreover, cotransfection of dominant negative mutants of MAP2K or MAPK blocked E 2 induction of leptin promoter. On the other hand, E2 treatment promoted MAPK1/MAPK3 and AKT phosphorylation in placental cells. In conclusion, we provide evidence suggesting that E2 induces leptin expression in trophoblastic cells, probably through genomic and nongenomic actions via crosstalk between estrogen receptor 1 and MAPK and PI3K signal transduction pathways. © 2010 by the Society for the Study of Reproduction, Inc. |
| format |
Artículo Artículo publishedVersion |
| author |
Gambino, Y.P. Maymó, J.L. Pérez-Pérez, A. Dueñas, J.L. Sánchez-Margalet, V. Calvo, J.C. Varone, C.L. |
| author_facet |
Gambino, Y.P. Maymó, J.L. Pérez-Pérez, A. Dueñas, J.L. Sánchez-Margalet, V. Calvo, J.C. Varone, C.L. |
| author_sort |
Gambino, Y.P. |
| title |
17Beta-estradiol enhances leptin expression in human placental cells through genomic and nongenomic actions |
| title_short |
17Beta-estradiol enhances leptin expression in human placental cells through genomic and nongenomic actions |
| title_full |
17Beta-estradiol enhances leptin expression in human placental cells through genomic and nongenomic actions |
| title_fullStr |
17Beta-estradiol enhances leptin expression in human placental cells through genomic and nongenomic actions |
| title_full_unstemmed |
17Beta-estradiol enhances leptin expression in human placental cells through genomic and nongenomic actions |
| title_sort |
17beta-estradiol enhances leptin expression in human placental cells through genomic and nongenomic actions |
| publishDate |
2010 |
| url |
http://hdl.handle.net/20.500.12110/paper_00063363_v83_n1_p42_Gambino |
| work_keys_str_mv |
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| _version_ |
1769810208117227520 |
| spelling |
paperaa:paper_00063363_v83_n1_p42_Gambino2023-06-12T16:41:17Z 17Beta-estradiol enhances leptin expression in human placental cells through genomic and nongenomic actions Biol. Reprod. 2010;83(1):42-51 Gambino, Y.P. Maymó, J.L. Pérez-Pérez, A. Dueñas, J.L. Sánchez-Margalet, V. Calvo, J.C. Varone, C.L. 17beta-estradiol Gene expression Leptin MAPK signal transduction pathway Placenta 2 (2 amino 3 methoxyphenyl)chromone estradiol estrogen receptor estrogen receptor alpha fulvestrant leptin luciferase mitogen activated protein kinase phosphatidylinositol 3 kinase wortmannin estradiol estrogen receptor alpha estrogen receptor alpha, human fulvestrant leptin mitogen activated protein kinase phosphatidylinositol 3 kinase protein kinase B adipocyte article cancer cell culture choriocarcinoma concentration response controlled study embryo enzyme inhibition enzyme phosphorylation explant gene deletion gene expression human human cell human tissue nidation placenta plasmid pregnancy priority journal promoter region reporter gene signal transduction transient transfection trophoblast Western blotting analogs and derivatives antagonists and inhibitors female in vitro study metabolism placenta signal transduction tumor cell line Bovinae Cell Line, Tumor Estradiol Estrogen Receptor alpha Extracellular Signal-Regulated MAP Kinases Female Humans In Vitro Techniques Leptin MAP Kinase Signaling System Phosphatidylinositol 3-Kinases Placenta Pregnancy Promoter Regions, Genetic Proto-Oncogene Proteins c-akt Receptor Cross-Talk The process of embryo implantation and trophoblast invasion is considered the most limiting factor in the establishment of pregnancy. Leptin was originally described as an adipocyte-derived signaling molecule for the central control of metabolism. However, it has been suggested that leptin is involved in other functions during pregnancy, particularly in the placenta, where it was found to be expressed. In the present work, we have found a stimulatory effect of 17beta-estradiol (E2) on endogenous leptin expression, as analyzed by Western blot, in both the BeWo choriocarcinoma cell line and normal placental explants. This effect was time and dose dependent. Maximal effect was achieved at 10 nM in BeWo cells and 1 nM in placental explants. The E 2 effects involved the estrogen receptor, as the antagonist ICI 182 780 inhibited E2-induced leptin expression. Moreover, E2 treatment enhanced leptin promoter activity up to 4-fold, as evaluated by transient transfection with a plasmid construction containing the leptin promoter region and the reporter gene luciferase. This effect was dose dependent. Deletion analysis demonstrated that a minimal promoter region between - 1951 and -1847 bp is both necessary and sufficient to achieve E2 effects. Estradiol action involved estrogen receptor 1, previously known as estrogen receptor alpha, as cotransfection with a vector encoding estrogen receptor 1 potentiated the effects of E2 on leptin expression. Moreover, E2 action probably involves membrane receptors too, as treatment with an estradiol-bovine serum albumin complex partially enhanced leptin expression. The effects of E2 could be blocked by pharmacologic inhibition of MAPK and the phosphoinositide-3-kinase (PI3K) pathways with 50 μM PD98059 and 0.1 μM Wortmannin, respectively. Moreover, cotransfection of dominant negative mutants of MAP2K or MAPK blocked E 2 induction of leptin promoter. On the other hand, E2 treatment promoted MAPK1/MAPK3 and AKT phosphorylation in placental cells. In conclusion, we provide evidence suggesting that E2 induces leptin expression in trophoblastic cells, probably through genomic and nongenomic actions via crosstalk between estrogen receptor 1 and MAPK and PI3K signal transduction pathways. © 2010 by the Society for the Study of Reproduction, Inc. Fil:Gambino, Y.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maymó, J.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Varone, C.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00063363_v83_n1_p42_Gambino |