Regulation of aryl hydrocarbon receptor expression in rat granulosa cells
The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that mediates most of the toxic and endocrine-disruptive actions of aromatic compounds in the ovary. Paradoxically, this receptor has been shown to play important roles in normal female reproductive function as well. Alth...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00063363_v75_n3_p360_Bussmann |
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paperaa:paper_00063363_v75_n3_p360_Bussmann2023-06-12T16:41:15Z Regulation of aryl hydrocarbon receptor expression in rat granulosa cells Biol. Reprod. 2006;75(3):360-369 Bussmann, U.A. Barañao, J.L. Estradiol Follicle-stimulating hormone Granulosa cells Ovary Toxicology aromatic compound aromatic hydrocarbon receptor cell receptor endocrine disruptor estradiol follitropin hormone ligand messenger RNA transcription factor animal cell article controlled study down regulation estrus cycle female female genital system granulosa cell hormonal regulation nonhuman ovary ovary cell culture physiology priority journal protein expression protein function rat receptor upregulation reproduction signal transduction Animals beta-Naphthoflavone Blotting, Western Cell Line Cytochrome P-450 CYP1A1 Down-Regulation Enzyme Inhibitors Estradiol Female Follicle Stimulating Hormone Gene Expression Regulation Granulosa Cells Ovary Proteasome Endopeptidase Complex Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon Reproduction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that mediates most of the toxic and endocrine-disruptive actions of aromatic compounds in the ovary. Paradoxically, this receptor has been shown to play important roles in normal female reproductive function as well. Although knowledge of AHR expression regulation in the ovary is of crucial significance to understand the receptor biology and its function in reproductive physiology, there are only limited data in this area. The purpose of the present study was to establish the possible regulation that AHR might undergo in ovarian cells. Here we show that the hormones FSH and estradiol are able to reduce AHR protein and transcript levels in granulosa cells in a way that parallels the changes observed in ovarian tissue across the rat estrous cycle. These findings suggest that estradiol and FSH would be cycle-associated endogenous modulators of AHR expression. In addition, we show that in granulosa cells the receptor is rapidly downregulated via proteasomal degradation following treatment with AHR ligands. However, prolonged treatment with an agonist caused an increase in Ahr mRNA levels. These actions would constitute a regulatory mechanism that both attenuates AHR signal rapidly and replenishes the cellular receptor pool in the long term. In conclusion, our results indicate that AHR expression is regulated by classical hormones and by its own ligands in granulosa cells. © 2006 by the Society for the Study of Reproduction, Inc. Fil:Bussmann, U.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, J.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00063363_v75_n3_p360_Bussmann |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
Estradiol Follicle-stimulating hormone Granulosa cells Ovary Toxicology aromatic compound aromatic hydrocarbon receptor cell receptor endocrine disruptor estradiol follitropin hormone ligand messenger RNA transcription factor animal cell article controlled study down regulation estrus cycle female female genital system granulosa cell hormonal regulation nonhuman ovary ovary cell culture physiology priority journal protein expression protein function rat receptor upregulation reproduction signal transduction Animals beta-Naphthoflavone Blotting, Western Cell Line Cytochrome P-450 CYP1A1 Down-Regulation Enzyme Inhibitors Estradiol Female Follicle Stimulating Hormone Gene Expression Regulation Granulosa Cells Ovary Proteasome Endopeptidase Complex Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon Reproduction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger |
| spellingShingle |
Estradiol Follicle-stimulating hormone Granulosa cells Ovary Toxicology aromatic compound aromatic hydrocarbon receptor cell receptor endocrine disruptor estradiol follitropin hormone ligand messenger RNA transcription factor animal cell article controlled study down regulation estrus cycle female female genital system granulosa cell hormonal regulation nonhuman ovary ovary cell culture physiology priority journal protein expression protein function rat receptor upregulation reproduction signal transduction Animals beta-Naphthoflavone Blotting, Western Cell Line Cytochrome P-450 CYP1A1 Down-Regulation Enzyme Inhibitors Estradiol Female Follicle Stimulating Hormone Gene Expression Regulation Granulosa Cells Ovary Proteasome Endopeptidase Complex Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon Reproduction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Bussmann, U.A. Barañao, J.L. Regulation of aryl hydrocarbon receptor expression in rat granulosa cells |
| topic_facet |
Estradiol Follicle-stimulating hormone Granulosa cells Ovary Toxicology aromatic compound aromatic hydrocarbon receptor cell receptor endocrine disruptor estradiol follitropin hormone ligand messenger RNA transcription factor animal cell article controlled study down regulation estrus cycle female female genital system granulosa cell hormonal regulation nonhuman ovary ovary cell culture physiology priority journal protein expression protein function rat receptor upregulation reproduction signal transduction Animals beta-Naphthoflavone Blotting, Western Cell Line Cytochrome P-450 CYP1A1 Down-Regulation Enzyme Inhibitors Estradiol Female Follicle Stimulating Hormone Gene Expression Regulation Granulosa Cells Ovary Proteasome Endopeptidase Complex Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon Reproduction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger |
| description |
The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that mediates most of the toxic and endocrine-disruptive actions of aromatic compounds in the ovary. Paradoxically, this receptor has been shown to play important roles in normal female reproductive function as well. Although knowledge of AHR expression regulation in the ovary is of crucial significance to understand the receptor biology and its function in reproductive physiology, there are only limited data in this area. The purpose of the present study was to establish the possible regulation that AHR might undergo in ovarian cells. Here we show that the hormones FSH and estradiol are able to reduce AHR protein and transcript levels in granulosa cells in a way that parallels the changes observed in ovarian tissue across the rat estrous cycle. These findings suggest that estradiol and FSH would be cycle-associated endogenous modulators of AHR expression. In addition, we show that in granulosa cells the receptor is rapidly downregulated via proteasomal degradation following treatment with AHR ligands. However, prolonged treatment with an agonist caused an increase in Ahr mRNA levels. These actions would constitute a regulatory mechanism that both attenuates AHR signal rapidly and replenishes the cellular receptor pool in the long term. In conclusion, our results indicate that AHR expression is regulated by classical hormones and by its own ligands in granulosa cells. © 2006 by the Society for the Study of Reproduction, Inc. |
| format |
Artículo Artículo publishedVersion |
| author |
Bussmann, U.A. Barañao, J.L. |
| author_facet |
Bussmann, U.A. Barañao, J.L. |
| author_sort |
Bussmann, U.A. |
| title |
Regulation of aryl hydrocarbon receptor expression in rat granulosa cells |
| title_short |
Regulation of aryl hydrocarbon receptor expression in rat granulosa cells |
| title_full |
Regulation of aryl hydrocarbon receptor expression in rat granulosa cells |
| title_fullStr |
Regulation of aryl hydrocarbon receptor expression in rat granulosa cells |
| title_full_unstemmed |
Regulation of aryl hydrocarbon receptor expression in rat granulosa cells |
| title_sort |
regulation of aryl hydrocarbon receptor expression in rat granulosa cells |
| publishDate |
2006 |
| url |
http://hdl.handle.net/20.500.12110/paper_00063363_v75_n3_p360_Bussmann |
| work_keys_str_mv |
AT bussmannua regulationofarylhydrocarbonreceptorexpressioninratgranulosacells AT baranaojl regulationofarylhydrocarbonreceptorexpressioninratgranulosacells |
| _version_ |
1769810311380992000 |