Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data

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In order to clarify the relationships among southern South American (sSA) representatives of the genus Ilex, an amplified fragment length polymorphism (AFLP) analysis was accomplished. In addition, the phylogenetic relationships of the species were studied using ribosomal internal transcribed spacer...

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Autores principales: Gottlieb, A.M., Giberti, G.C., Poggio, L.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2005
Materias:
AFLP
Direct optimization
Ilex
ITS
Sensitivity analysis
molecular analysis
sensitivity analysis
taxonomy
Aquifoliaceae
Embryophyta
Ilex argentina
Ilex brasiliensis
Ilex brevicuspis
Ilex dumosa
Ilex integerrima
Ilex paraguariensis
Ilex theezans
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00029122_v92_n2_p352_Gottlieb
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling paperaa:paper_00029122_v92_n2_p352_Gottlieb2023-06-12T16:39:36Z Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data Am. J. Bot. 2005;92(2):352-369 Gottlieb, A.M. Giberti, G.C. Poggio, L. AFLP Direct optimization Ilex ITS Sensitivity analysis molecular analysis sensitivity analysis taxonomy Aquifoliaceae Embryophyta Ilex Ilex Ilex argentina Ilex argentina Ilex brasiliensis Ilex brasiliensis Ilex brevicuspis Ilex brevicuspis Ilex dumosa Ilex dumosa Ilex integerrima Ilex integerrima Ilex paraguariensis Ilex paraguariensis Ilex theezans Ilex theezans In order to clarify the relationships among southern South American (sSA) representatives of the genus Ilex, an amplified fragment length polymorphism (AFLP) analysis was accomplished. In addition, the phylogenetic relationships of the species were studied using ribosomal internal transcribed spacer (ITS) sequence data alone and in combination with AFLP data, taking into account the possible existence of paralogous sequences and the influence of alignment parameters. To explore stability of phylogenetic hypotheses, a sensitivity analysis was performed using 15 indel-substitution models. Within each species assayed, the AFLPs allowed the recognition of several diagnostic bands. Furthermore, the AFLP analysis revealed that individuals belonging to the same morpho-species formed coherent clades. In addition, some cases of geographical association were noted. Studies on ITS sequences revealed divergence between data obtained herein and sequence data downloaded from GenBank. The sensitivity analyses yielded different interspecific hypotheses of relationships. Notwithstanding, analyses of the ITS data alone and in combination with AFLPs, rendered clades stable to variation in the analytical parameters. Topologies obtained for the AFLPs, the ITS data alone and the combined analyses, demonstrated the existence of a group formed by I. argentina, I. brasiliensis, I. brevicuspis, I. integerrima, and I. theezans, and that I. dumosa and I. paraguariensis were distantly related to the former. Incongruence with traditional taxonomical treatments was found. Fil:Gottlieb, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00029122_v92_n2_p352_Gottlieb
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic AFLP
Direct optimization
Ilex
ITS
Sensitivity analysis
molecular analysis
sensitivity analysis
taxonomy
Aquifoliaceae
Embryophyta
Ilex
Ilex
Ilex argentina
Ilex argentina
Ilex brasiliensis
Ilex brasiliensis
Ilex brevicuspis
Ilex brevicuspis
Ilex dumosa
Ilex dumosa
Ilex integerrima
Ilex integerrima
Ilex paraguariensis
Ilex paraguariensis
Ilex theezans
Ilex theezans
spellingShingle AFLP
Direct optimization
Ilex
ITS
Sensitivity analysis
molecular analysis
sensitivity analysis
taxonomy
Aquifoliaceae
Embryophyta
Ilex
Ilex
Ilex argentina
Ilex argentina
Ilex brasiliensis
Ilex brasiliensis
Ilex brevicuspis
Ilex brevicuspis
Ilex dumosa
Ilex dumosa
Ilex integerrima
Ilex integerrima
Ilex paraguariensis
Ilex paraguariensis
Ilex theezans
Ilex theezans
Gottlieb, A.M.
Giberti, G.C.
Poggio, L.
Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data
topic_facet AFLP
Direct optimization
Ilex
ITS
Sensitivity analysis
molecular analysis
sensitivity analysis
taxonomy
Aquifoliaceae
Embryophyta
Ilex
Ilex
Ilex argentina
Ilex argentina
Ilex brasiliensis
Ilex brasiliensis
Ilex brevicuspis
Ilex brevicuspis
Ilex dumosa
Ilex dumosa
Ilex integerrima
Ilex integerrima
Ilex paraguariensis
Ilex paraguariensis
Ilex theezans
Ilex theezans
description In order to clarify the relationships among southern South American (sSA) representatives of the genus Ilex, an amplified fragment length polymorphism (AFLP) analysis was accomplished. In addition, the phylogenetic relationships of the species were studied using ribosomal internal transcribed spacer (ITS) sequence data alone and in combination with AFLP data, taking into account the possible existence of paralogous sequences and the influence of alignment parameters. To explore stability of phylogenetic hypotheses, a sensitivity analysis was performed using 15 indel-substitution models. Within each species assayed, the AFLPs allowed the recognition of several diagnostic bands. Furthermore, the AFLP analysis revealed that individuals belonging to the same morpho-species formed coherent clades. In addition, some cases of geographical association were noted. Studies on ITS sequences revealed divergence between data obtained herein and sequence data downloaded from GenBank. The sensitivity analyses yielded different interspecific hypotheses of relationships. Notwithstanding, analyses of the ITS data alone and in combination with AFLPs, rendered clades stable to variation in the analytical parameters. Topologies obtained for the AFLPs, the ITS data alone and the combined analyses, demonstrated the existence of a group formed by I. argentina, I. brasiliensis, I. brevicuspis, I. integerrima, and I. theezans, and that I. dumosa and I. paraguariensis were distantly related to the former. Incongruence with traditional taxonomical treatments was found.
format Artículo
Artículo
publishedVersion
author Gottlieb, A.M.
Giberti, G.C.
Poggio, L.
author_facet Gottlieb, A.M.
Giberti, G.C.
Poggio, L.
author_sort Gottlieb, A.M.
title Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data
title_short Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data
title_full Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data
title_fullStr Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data
title_full_unstemmed Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data
title_sort molecular analyses of the genus ilex (aquifoliaceae) in southern south america, evidence from aflp and its sequence data
publishDate 2005
url http://hdl.handle.net/20.500.12110/paper_00029122_v92_n2_p352_Gottlieb
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AT poggiol molecularanalysesofthegenusilexaquifoliaceaeinsouthernsouthamericaevidencefromaflpanditssequencedata
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