Transcriptional features of fractionated human breast tumor chromatin

By means of controlled mechanical shearing it was possible to fractionate human breast tumor chromatin in 'active' and 'inactive' species, according to their in vitro template activity, using E. coli RNA polymerase. The 'active' molecules can be resolved in 3 well defin...

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Guardado en:
Detalles Bibliográficos
Publicado: 1979
Materias:
rna
rna polymerase
breast
breast cancer
chromatin
escherichia coli
euchromatin
genetic transcription
heterochromatin
human cell
in vitro study
theoretical study
Breast Neoplasms
Chromatin
Deoxyribonucleoproteins
DNA, Neoplasm
Female
Human
RNA Polymerase I
RNA, Neoplasm
Support, Non-U.S. Gov't
Transcription, Genetic
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_NIS18558_v29_n1_p81_Thierbach
http://hdl.handle.net/20.500.12110/paper_NIS18558_v29_n1_p81_Thierbach
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:By means of controlled mechanical shearing it was possible to fractionate human breast tumor chromatin in 'active' and 'inactive' species, according to their in vitro template activity, using E. coli RNA polymerase. The 'active' molecules can be resolved in 3 well defined regions in an exponential sucrose gradient, showing approximately 300 times the efficiency for synthesizing ribonucleic acid, relative to the chromatin which migrated fastest. This technique could provide the initial tool to isolate eu-and heterochromatin from native human chromatin.

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