Transcriptional features of fractionated human breast tumor chromatin

By means of controlled mechanical shearing it was possible to fractionate human breast tumor chromatin in 'active' and 'inactive' species, according to their in vitro template activity, using E. coli RNA polymerase. The 'active' molecules can be resolved in 3 well defin...

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Detalles Bibliográficos
Publicado: 1979
Materias:
rna
rna polymerase
breast
breast cancer
chromatin
escherichia coli
euchromatin
genetic transcription
heterochromatin
human cell
in vitro study
theoretical study
Breast Neoplasms
Chromatin
Deoxyribonucleoproteins
DNA, Neoplasm
Female
Human
RNA Polymerase I
RNA, Neoplasm
Support, Non-U.S. Gov't
Transcription, Genetic
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_NIS18558_v29_n1_p81_Thierbach
http://hdl.handle.net/20.500.12110/paper_NIS18558_v29_n1_p81_Thierbach
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_NIS18558_v29_n1_p81_Thierbach
record_format dspace
spelling paper:paper_NIS18558_v29_n1_p81_Thierbach2023-06-08T16:39:46Z Transcriptional features of fractionated human breast tumor chromatin rna rna polymerase breast breast cancer chromatin escherichia coli euchromatin genetic transcription heterochromatin human cell in vitro study theoretical study Breast Neoplasms Chromatin Deoxyribonucleoproteins DNA, Neoplasm Female Human RNA Polymerase I RNA, Neoplasm Support, Non-U.S. Gov't Transcription, Genetic By means of controlled mechanical shearing it was possible to fractionate human breast tumor chromatin in 'active' and 'inactive' species, according to their in vitro template activity, using E. coli RNA polymerase. The 'active' molecules can be resolved in 3 well defined regions in an exponential sucrose gradient, showing approximately 300 times the efficiency for synthesizing ribonucleic acid, relative to the chromatin which migrated fastest. This technique could provide the initial tool to isolate eu-and heterochromatin from native human chromatin. 1979 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_NIS18558_v29_n1_p81_Thierbach http://hdl.handle.net/20.500.12110/paper_NIS18558_v29_n1_p81_Thierbach
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic rna
rna polymerase
breast
breast cancer
chromatin
escherichia coli
euchromatin
genetic transcription
heterochromatin
human cell
in vitro study
theoretical study
Breast Neoplasms
Chromatin
Deoxyribonucleoproteins
DNA, Neoplasm
Female
Human
RNA Polymerase I
RNA, Neoplasm
Support, Non-U.S. Gov't
Transcription, Genetic
spellingShingle rna
rna polymerase
breast
breast cancer
chromatin
escherichia coli
euchromatin
genetic transcription
heterochromatin
human cell
in vitro study
theoretical study
Breast Neoplasms
Chromatin
Deoxyribonucleoproteins
DNA, Neoplasm
Female
Human
RNA Polymerase I
RNA, Neoplasm
Support, Non-U.S. Gov't
Transcription, Genetic
Transcriptional features of fractionated human breast tumor chromatin
topic_facet rna
rna polymerase
breast
breast cancer
chromatin
escherichia coli
euchromatin
genetic transcription
heterochromatin
human cell
in vitro study
theoretical study
Breast Neoplasms
Chromatin
Deoxyribonucleoproteins
DNA, Neoplasm
Female
Human
RNA Polymerase I
RNA, Neoplasm
Support, Non-U.S. Gov't
Transcription, Genetic
description By means of controlled mechanical shearing it was possible to fractionate human breast tumor chromatin in 'active' and 'inactive' species, according to their in vitro template activity, using E. coli RNA polymerase. The 'active' molecules can be resolved in 3 well defined regions in an exponential sucrose gradient, showing approximately 300 times the efficiency for synthesizing ribonucleic acid, relative to the chromatin which migrated fastest. This technique could provide the initial tool to isolate eu-and heterochromatin from native human chromatin.
title Transcriptional features of fractionated human breast tumor chromatin
title_short Transcriptional features of fractionated human breast tumor chromatin
title_full Transcriptional features of fractionated human breast tumor chromatin
title_fullStr Transcriptional features of fractionated human breast tumor chromatin
title_full_unstemmed Transcriptional features of fractionated human breast tumor chromatin
title_sort transcriptional features of fractionated human breast tumor chromatin
publishDate 1979
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_NIS18558_v29_n1_p81_Thierbach
http://hdl.handle.net/20.500.12110/paper_NIS18558_v29_n1_p81_Thierbach
_version_ 1769175826401591296

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