Coupled Promoter Splicing Systems

In this chapter are reported the experimental strategies and details to assess alternative splicing (AS) in mammalian cells that are transfected with reporter minigenes carrying different promoters, or where artificial mutants of RNA polymerase II are used to generate RNA from these reporters. These...

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Autores principales: Muñoz, Manuel Javier, de la Mata, Manuel, Kornblihtt, Alberto Rodolfo
Publicado: 2012
Materias:
Coupling
Reporter minigene transfections
RT-PCR
Transcription and alternative splicing
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97835273_v_n_p400_Munoz
http://hdl.handle.net/20.500.12110/paper_97835273_v_n_p400_Munoz
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_97835273_v_n_p400_Munoz
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spelling paper:paper_97835273_v_n_p400_Munoz2025-07-30T19:14:43Z Coupled Promoter Splicing Systems Muñoz, Manuel Javier de la Mata, Manuel Kornblihtt, Alberto Rodolfo Coupling Reporter minigene transfections RT-PCR Transcription and alternative splicing In this chapter are reported the experimental strategies and details to assess alternative splicing (AS) in mammalian cells that are transfected with reporter minigenes carrying different promoters, or where artificial mutants of RNA polymerase II are used to generate RNA from these reporters. These techniques were applied successfully to demonstrate the coupling between transcription and AS, and to elucidate the roles of the carboxy-terminal domain of RNA polymerase II in the recruitment of splicing factors, and of the rate of transcriptional elongation on splice site selection. © 2012 Wiley-VCH Verlag GmbH & Co. KGaA. Fil:Muñoz, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:de la Mata, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97835273_v_n_p400_Munoz http://hdl.handle.net/20.500.12110/paper_97835273_v_n_p400_Munoz
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Coupling
Reporter minigene transfections
RT-PCR
Transcription and alternative splicing
spellingShingle Coupling
Reporter minigene transfections
RT-PCR
Transcription and alternative splicing
Muñoz, Manuel Javier
de la Mata, Manuel
Kornblihtt, Alberto Rodolfo
Coupled Promoter Splicing Systems
topic_facet Coupling
Reporter minigene transfections
RT-PCR
Transcription and alternative splicing
description In this chapter are reported the experimental strategies and details to assess alternative splicing (AS) in mammalian cells that are transfected with reporter minigenes carrying different promoters, or where artificial mutants of RNA polymerase II are used to generate RNA from these reporters. These techniques were applied successfully to demonstrate the coupling between transcription and AS, and to elucidate the roles of the carboxy-terminal domain of RNA polymerase II in the recruitment of splicing factors, and of the rate of transcriptional elongation on splice site selection. © 2012 Wiley-VCH Verlag GmbH & Co. KGaA.
author Muñoz, Manuel Javier
de la Mata, Manuel
Kornblihtt, Alberto Rodolfo
author_facet Muñoz, Manuel Javier
de la Mata, Manuel
Kornblihtt, Alberto Rodolfo
author_sort Muñoz, Manuel Javier
title Coupled Promoter Splicing Systems
title_short Coupled Promoter Splicing Systems
title_full Coupled Promoter Splicing Systems
title_fullStr Coupled Promoter Splicing Systems
title_full_unstemmed Coupled Promoter Splicing Systems
title_sort coupled promoter splicing systems
publishDate 2012
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97835273_v_n_p400_Munoz
http://hdl.handle.net/20.500.12110/paper_97835273_v_n_p400_Munoz
work_keys_str_mv AT munozmanueljavier coupledpromotersplicingsystems
AT delamatamanuel coupledpromotersplicingsystems
AT kornblihttalbertorodolfo coupledpromotersplicingsystems
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