In vitro and in vivo 5-aminolevulinic acid-based photodynamic therapy in sensitive ani drug resistant leukemic murine cells

The effects of combined administration of doxorubicin (DOX) and vincristine (VCR), with 5-aminolevulinic acid photodynamic treatment (ALA-PDT), were analyzed in sensitive murine leukemic cell lines (LBR-) and DOX and VCR chemoresistant LBR-D160 and LBR-V160 cell lines. Low doses of DOX and VCR incre...

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Autores principales: Diez, Berenice Andrea, Ernst, Glenda M., Teijo, María Julieta, Batlle, Alcira María del Carmen, Fukuda, Haydeé
Publicado: 2015
Materias:
5-aminolevulinic acid
Chemotherapy resistance
Leukemic cells
Photodynamic therapy
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97816346_v_n_p173_Diez
http://hdl.handle.net/20.500.12110/paper_97816346_v_n_p173_Diez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_97816346_v_n_p173_Diez
record_format dspace
spelling paper:paper_97816346_v_n_p173_Diez2023-06-08T16:38:33Z In vitro and in vivo 5-aminolevulinic acid-based photodynamic therapy in sensitive ani drug resistant leukemic murine cells Diez, Berenice Andrea Ernst, Glenda M. Teijo, María Julieta Batlle, Alcira María del Carmen Fukuda, Haydeé 5-aminolevulinic acid Chemotherapy resistance Leukemic cells Photodynamic therapy The effects of combined administration of doxorubicin (DOX) and vincristine (VCR), with 5-aminolevulinic acid photodynamic treatment (ALA-PDT), were analyzed in sensitive murine leukemic cell lines (LBR-) and DOX and VCR chemoresistant LBR-D160 and LBR-V160 cell lines. Low doses of DOX and VCR increased anti-cancer effect of ALA-PDT in LBR-cells. Decrease in cell survival was higher when the combination VCR + ALA-PDT was used compared to DOX + ALA-PDT. Resistant cell lines LBR-D160 and LBR-V160 were sensitive to ALA-PDT; however, no changes occured when combining therapies. Thus, ALA-PDT can overcome drug resistance and is a good candidate for using treating multidrug resistant (MDR) cells. This combined treatment were evaluated in a murine model. For this purpose, BALB/c mice were inoculated with LBR-cells previously treated with DOX or VCR plus ALA-PDT. 30 days after treatment animals were sacrificed and tumoral infiltration analyzed in many tissues: liver, kidney, spleen, lung, brain, timus, lymph nodes. Histologic studies revealed that in control animals inoculated with LBR-cells without any treatment, infiltration reached 87% in all the tissues analyzed. Animals inoculated with LBR-cells treated with only ALA-PDT, showed 50% of organs infiltrated. When animals were inoculated with cells treated with DOX or VCR, tumor infiltration was found in 87.5% and 75% of organs, respectively. In the case of inoculation with LBR-cells treated with DOX or VCR plus ALA-PDT, no evidence of tumor infiltration was observed in any of the tissues. These results show the beneficial effect of combining therapies, suggesting the potential therapeutic alternative in leukemic patients, additionaly bringing the possibility of diminishing chemotherapy dose, thus minimizing undesirable drug side effects. © 2015 by Nova Science Publishers, Inc. All rights reserved. Fil:Diez, B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ernst, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Teijo, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fukuda, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97816346_v_n_p173_Diez http://hdl.handle.net/20.500.12110/paper_97816346_v_n_p173_Diez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 5-aminolevulinic acid
Chemotherapy resistance
Leukemic cells
Photodynamic therapy
spellingShingle 5-aminolevulinic acid
Chemotherapy resistance
Leukemic cells
Photodynamic therapy
Diez, Berenice Andrea
Ernst, Glenda M.
Teijo, María Julieta
Batlle, Alcira María del Carmen
Fukuda, Haydeé
In vitro and in vivo 5-aminolevulinic acid-based photodynamic therapy in sensitive ani drug resistant leukemic murine cells
topic_facet 5-aminolevulinic acid
Chemotherapy resistance
Leukemic cells
Photodynamic therapy
description The effects of combined administration of doxorubicin (DOX) and vincristine (VCR), with 5-aminolevulinic acid photodynamic treatment (ALA-PDT), were analyzed in sensitive murine leukemic cell lines (LBR-) and DOX and VCR chemoresistant LBR-D160 and LBR-V160 cell lines. Low doses of DOX and VCR increased anti-cancer effect of ALA-PDT in LBR-cells. Decrease in cell survival was higher when the combination VCR + ALA-PDT was used compared to DOX + ALA-PDT. Resistant cell lines LBR-D160 and LBR-V160 were sensitive to ALA-PDT; however, no changes occured when combining therapies. Thus, ALA-PDT can overcome drug resistance and is a good candidate for using treating multidrug resistant (MDR) cells. This combined treatment were evaluated in a murine model. For this purpose, BALB/c mice were inoculated with LBR-cells previously treated with DOX or VCR plus ALA-PDT. 30 days after treatment animals were sacrificed and tumoral infiltration analyzed in many tissues: liver, kidney, spleen, lung, brain, timus, lymph nodes. Histologic studies revealed that in control animals inoculated with LBR-cells without any treatment, infiltration reached 87% in all the tissues analyzed. Animals inoculated with LBR-cells treated with only ALA-PDT, showed 50% of organs infiltrated. When animals were inoculated with cells treated with DOX or VCR, tumor infiltration was found in 87.5% and 75% of organs, respectively. In the case of inoculation with LBR-cells treated with DOX or VCR plus ALA-PDT, no evidence of tumor infiltration was observed in any of the tissues. These results show the beneficial effect of combining therapies, suggesting the potential therapeutic alternative in leukemic patients, additionaly bringing the possibility of diminishing chemotherapy dose, thus minimizing undesirable drug side effects. © 2015 by Nova Science Publishers, Inc. All rights reserved.
author Diez, Berenice Andrea
Ernst, Glenda M.
Teijo, María Julieta
Batlle, Alcira María del Carmen
Fukuda, Haydeé
author_facet Diez, Berenice Andrea
Ernst, Glenda M.
Teijo, María Julieta
Batlle, Alcira María del Carmen
Fukuda, Haydeé
author_sort Diez, Berenice Andrea
title In vitro and in vivo 5-aminolevulinic acid-based photodynamic therapy in sensitive ani drug resistant leukemic murine cells
title_short In vitro and in vivo 5-aminolevulinic acid-based photodynamic therapy in sensitive ani drug resistant leukemic murine cells
title_full In vitro and in vivo 5-aminolevulinic acid-based photodynamic therapy in sensitive ani drug resistant leukemic murine cells
title_fullStr In vitro and in vivo 5-aminolevulinic acid-based photodynamic therapy in sensitive ani drug resistant leukemic murine cells
title_full_unstemmed In vitro and in vivo 5-aminolevulinic acid-based photodynamic therapy in sensitive ani drug resistant leukemic murine cells
title_sort in vitro and in vivo 5-aminolevulinic acid-based photodynamic therapy in sensitive ani drug resistant leukemic murine cells
publishDate 2015
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97816346_v_n_p173_Diez
http://hdl.handle.net/20.500.12110/paper_97816346_v_n_p173_Diez
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