Small photostable photoswitchable quantum dots as nanotools for live cell imaging

We recently reported a novel surface architecture for the generation of biocompatible and stable photoswitchable quantum dots (psQDs). The system is based on Förster Resonance Energy Transfer (FRET) from a QD donor to diheteroarylethenes photochromic (PC) acceptors (pcFRET). The PC molecules are lin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Jares, Elizabeth Andrea
Publicado:	2011
Materias:	EGFR FRET Imaging Photochromic Quantum dots Amphiphilic polymers Carboxyl groups Epidermal growth factor receptors Live cell Live-cell imaging Nanotools Novel surfaces Over-expression Photo-switchable Photostable Quantum Dot Resonance energy transfer Time-resolved fluorescence Energy transfer Fluidics Imaging techniques Medical imaging Medical nanotechnology Microcomputers Nanotechnology Photochromism Semiconductor quantum dots
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97814398_v3_n_p205_Diaz http://hdl.handle.net/20.500.12110/paper_97814398_v3_n_p205_Diaz
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_97814398_v3_n_p205_Diaz
record_format	dspace
spelling	paper:paper_97814398_v3_n_p205_Diaz2023-06-08T16:37:26Z Small photostable photoswitchable quantum dots as nanotools for live cell imaging Jares, Elizabeth Andrea EGFR FRET Imaging Photochromic Quantum dots Amphiphilic polymers Carboxyl groups EGFR Epidermal growth factor receptors FRET Live cell Live-cell imaging Nanotools Novel surfaces Over-expression Photo-switchable Photochromic Photostable Quantum Dot Resonance energy transfer Time-resolved fluorescence Energy transfer Fluidics Imaging techniques Medical imaging Medical nanotechnology Microcomputers Nanotechnology Photochromism Semiconductor quantum dots We recently reported a novel surface architecture for the generation of biocompatible and stable photoswitchable quantum dots (psQDs). The system is based on Förster Resonance Energy Transfer (FRET) from a QD donor to diheteroarylethenes photochromic (PC) acceptors (pcFRET). The PC molecules are linked to an amphiphilic polymer that self-assembles, creating an interior lipophilic space, in which the hydrophobic PCs are included, and external facing carboxyl groups. psQDs retain the desirable properties of the original QDs; furthermore the brightness can be tailored by light. The modulation of emission monitored by steady-state and time-resolved fluorescence is 35-40%. The epidermal growth factor receptor (EGFR) is of far-reaching biomedical importance due to its overexpression and/or mutation in various types of cancer. Here, we demonstrate imaging of live cells exposed to psQDs targeted to the EGFR. Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97814398_v3_n_p205_Diaz http://hdl.handle.net/20.500.12110/paper_97814398_v3_n_p205_Diaz
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	EGFR FRET Imaging Photochromic Quantum dots Amphiphilic polymers Carboxyl groups EGFR Epidermal growth factor receptors FRET Live cell Live-cell imaging Nanotools Novel surfaces Over-expression Photo-switchable Photochromic Photostable Quantum Dot Resonance energy transfer Time-resolved fluorescence Energy transfer Fluidics Imaging techniques Medical imaging Medical nanotechnology Microcomputers Nanotechnology Photochromism Semiconductor quantum dots
spellingShingle	EGFR FRET Imaging Photochromic Quantum dots Amphiphilic polymers Carboxyl groups EGFR Epidermal growth factor receptors FRET Live cell Live-cell imaging Nanotools Novel surfaces Over-expression Photo-switchable Photochromic Photostable Quantum Dot Resonance energy transfer Time-resolved fluorescence Energy transfer Fluidics Imaging techniques Medical imaging Medical nanotechnology Microcomputers Nanotechnology Photochromism Semiconductor quantum dots Jares, Elizabeth Andrea Small photostable photoswitchable quantum dots as nanotools for live cell imaging
topic_facet	EGFR FRET Imaging Photochromic Quantum dots Amphiphilic polymers Carboxyl groups EGFR Epidermal growth factor receptors FRET Live cell Live-cell imaging Nanotools Novel surfaces Over-expression Photo-switchable Photochromic Photostable Quantum Dot Resonance energy transfer Time-resolved fluorescence Energy transfer Fluidics Imaging techniques Medical imaging Medical nanotechnology Microcomputers Nanotechnology Photochromism Semiconductor quantum dots
description	We recently reported a novel surface architecture for the generation of biocompatible and stable photoswitchable quantum dots (psQDs). The system is based on Förster Resonance Energy Transfer (FRET) from a QD donor to diheteroarylethenes photochromic (PC) acceptors (pcFRET). The PC molecules are linked to an amphiphilic polymer that self-assembles, creating an interior lipophilic space, in which the hydrophobic PCs are included, and external facing carboxyl groups. psQDs retain the desirable properties of the original QDs; furthermore the brightness can be tailored by light. The modulation of emission monitored by steady-state and time-resolved fluorescence is 35-40%. The epidermal growth factor receptor (EGFR) is of far-reaching biomedical importance due to its overexpression and/or mutation in various types of cancer. Here, we demonstrate imaging of live cells exposed to psQDs targeted to the EGFR.
author	Jares, Elizabeth Andrea
author_facet	Jares, Elizabeth Andrea
author_sort	Jares, Elizabeth Andrea
title	Small photostable photoswitchable quantum dots as nanotools for live cell imaging
title_short	Small photostable photoswitchable quantum dots as nanotools for live cell imaging
title_full	Small photostable photoswitchable quantum dots as nanotools for live cell imaging
title_fullStr	Small photostable photoswitchable quantum dots as nanotools for live cell imaging
title_full_unstemmed	Small photostable photoswitchable quantum dots as nanotools for live cell imaging
title_sort	small photostable photoswitchable quantum dots as nanotools for live cell imaging
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97814398_v3_n_p205_Diaz http://hdl.handle.net/20.500.12110/paper_97814398_v3_n_p205_Diaz
work_keys_str_mv	AT jareselizabethandrea smallphotostablephotoswitchablequantumdotsasnanotoolsforlivecellimaging
_version_	1768542290816532480

Small photostable photoswitchable quantum dots as nanotools for live cell imaging

Ejemplares similares