Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina
Neobalantidium coli (Pomajbíková et al., 2013) is a cosmopolitan ciliate which colonizes the intestine of humans and animals. Pigs are the most important host and reservoir for this parasite, although others mammals have been described. Humans can acquire the disease through the ingestion of water a...
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2017
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_24059390_v10_n_p114_LopezArias http://hdl.handle.net/20.500.12110/paper_24059390_v10_n_p114_LopezArias |
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paper:paper_24059390_v10_n_p114_LopezArias2023-06-08T16:35:56Z Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina 18S rRNA Human balantidiosis Neobalantidium coli PCR Swine balantidiosis Zoonotic transmission RNA 18S animal experiment animal health Argentina Article balantidiasis controlled study cyst disease transmission DNA extraction feces health care planning microscopy mortality Neobalantidium coli nonhuman parasite pig polymerase chain reaction zoonosis Neobalantidium coli (Pomajbíková et al., 2013) is a cosmopolitan ciliate which colonizes the intestine of humans and animals. Pigs are the most important host and reservoir for this parasite, although others mammals have been described. Humans can acquire the disease through the ingestion of water and food contaminated with cysts and even from person to person contact. Farmers and slaughterhouse workers from rural areas of developing countries have an increased incidence of balantidiosis. In Argentina, despite swine production on family farms covers 70% of domestic consumption requirements; there is a lack of veterinary animal health planning which result in high rate of animal mortality, as well as environmental risk due to inefficient facilities and mismanagement of manure and effluents. At present there are no epidemiological data on balantidiosis in Argentina, except for isolated reports. Therefore, the aims of this study were to establish the frequency of N. coli in pigs raised under different conditions and to explore the zoonotic potential. In order to confirm the identity of Neobalantidium coli like-cysts founded in the feces, a set of N. coli specific primers based on 18S rRNA gene sequences was designed. The molecular identification of N. coli was performed in 88.9% (16 out of 18) of swine stool samples in which cysts had been visualized. The fecal samples obtained from pigs raised on more open farmland showed a lower percentage of N. coli than those obtained from animals raised in swine pens. On the other hand, molecular identification of N. coli was also performed in human feces. Pairwise comparison of sequences obtained from pigs and human fecal samples from the NW Region of Argentina showed a high percentage of similarity, indicating a possible zoonotic transmission. © 2017 Elsevier B.V. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_24059390_v10_n_p114_LopezArias http://hdl.handle.net/20.500.12110/paper_24059390_v10_n_p114_LopezArias |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
18S rRNA Human balantidiosis Neobalantidium coli PCR Swine balantidiosis Zoonotic transmission RNA 18S animal experiment animal health Argentina Article balantidiasis controlled study cyst disease transmission DNA extraction feces health care planning microscopy mortality Neobalantidium coli nonhuman parasite pig polymerase chain reaction zoonosis |
| spellingShingle |
18S rRNA Human balantidiosis Neobalantidium coli PCR Swine balantidiosis Zoonotic transmission RNA 18S animal experiment animal health Argentina Article balantidiasis controlled study cyst disease transmission DNA extraction feces health care planning microscopy mortality Neobalantidium coli nonhuman parasite pig polymerase chain reaction zoonosis Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina |
| topic_facet |
18S rRNA Human balantidiosis Neobalantidium coli PCR Swine balantidiosis Zoonotic transmission RNA 18S animal experiment animal health Argentina Article balantidiasis controlled study cyst disease transmission DNA extraction feces health care planning microscopy mortality Neobalantidium coli nonhuman parasite pig polymerase chain reaction zoonosis |
| description |
Neobalantidium coli (Pomajbíková et al., 2013) is a cosmopolitan ciliate which colonizes the intestine of humans and animals. Pigs are the most important host and reservoir for this parasite, although others mammals have been described. Humans can acquire the disease through the ingestion of water and food contaminated with cysts and even from person to person contact. Farmers and slaughterhouse workers from rural areas of developing countries have an increased incidence of balantidiosis. In Argentina, despite swine production on family farms covers 70% of domestic consumption requirements; there is a lack of veterinary animal health planning which result in high rate of animal mortality, as well as environmental risk due to inefficient facilities and mismanagement of manure and effluents. At present there are no epidemiological data on balantidiosis in Argentina, except for isolated reports. Therefore, the aims of this study were to establish the frequency of N. coli in pigs raised under different conditions and to explore the zoonotic potential. In order to confirm the identity of Neobalantidium coli like-cysts founded in the feces, a set of N. coli specific primers based on 18S rRNA gene sequences was designed. The molecular identification of N. coli was performed in 88.9% (16 out of 18) of swine stool samples in which cysts had been visualized. The fecal samples obtained from pigs raised on more open farmland showed a lower percentage of N. coli than those obtained from animals raised in swine pens. On the other hand, molecular identification of N. coli was also performed in human feces. Pairwise comparison of sequences obtained from pigs and human fecal samples from the NW Region of Argentina showed a high percentage of similarity, indicating a possible zoonotic transmission. © 2017 Elsevier B.V. |
| title |
Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina |
| title_short |
Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina |
| title_full |
Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina |
| title_fullStr |
Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina |
| title_full_unstemmed |
Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina |
| title_sort |
development of a pcr assay for identification of neobalantidium coli (pomajbíková et al., 2013) in argentina |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_24059390_v10_n_p114_LopezArias http://hdl.handle.net/20.500.12110/paper_24059390_v10_n_p114_LopezArias |
| _version_ |
1768544843487772672 |