Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro

Polyfunctionalized stigmasterol derivatives, (22S,23S)-22,23-dihydroxystigmast-4-en-3-one (compound 1) and (22S,23S)-3β-bromo-5,22,23-trihydroxystigmastan-6-one (compound 2), inhibit herpes simplex virus type 1 (HSV-1) replication and spreading in human epithelial cells derived from ocular tissues....

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Autores principales:	Petrera, Erina, Níttolo, Analía Gabriela, Alche, Laura Edith
Publicado:	2014
Materias:	22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one gamma interferon interleukin 6 stigmasterol unclassified drug 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one antiinflammatory agent antivirus agent cholestane derivative animal cell antiinflammatory activity antiviral activity Article controlled study cytokine production cytotoxicity assay herpes simplex keratitis Herpes simplex virus 1 human human cell immunopathology interferon production nerve cell nonhuman virus inhibition virus replication analogs and derivatives animal cell line Chlorocebus aethiops drug effects epithelium cell inflammation metabolism mouse Vero cell line Human herpesvirus 1 Murinae Simplexvirus Animals Anti-Inflammatory Agents Antiviral Agents Cell Line Cercopithecus aethiops Cholestanones Epithelial Cells Herpesvirus 1, Human Humans Inflammation Interferon-gamma Interleukin-6 Mice Stigmasterol Vero Cells Virus Replication
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_23146133_v2014_n_p_Petrera http://hdl.handle.net/20.500.12110/paper_23146133_v2014_n_p_Petrera
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_23146133_v2014_n_p_Petrera
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spelling	paper:paper_23146133_v2014_n_p_Petrera2023-06-08T16:35:34Z Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro Petrera, Erina Níttolo, Analía Gabriela Alche, Laura Edith 22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one gamma interferon interleukin 6 stigmasterol unclassified drug 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one antiinflammatory agent antivirus agent cholestane derivative gamma interferon interleukin 6 stigmasterol animal cell antiinflammatory activity antiviral activity Article controlled study cytokine production cytotoxicity assay herpes simplex keratitis Herpes simplex virus 1 human human cell immunopathology interferon production nerve cell nonhuman virus inhibition virus replication analogs and derivatives animal cell line Chlorocebus aethiops drug effects epithelium cell Herpes simplex virus 1 inflammation metabolism mouse Vero cell line virus replication Human herpesvirus 1 Murinae Simplexvirus Animals Anti-Inflammatory Agents Antiviral Agents Cell Line Cercopithecus aethiops Cholestanones Epithelial Cells Herpesvirus 1, Human Humans Inflammation Interferon-gamma Interleukin-6 Mice Stigmasterol Vero Cells Virus Replication Polyfunctionalized stigmasterol derivatives, (22S,23S)-22,23-dihydroxystigmast-4-en-3-one (compound 1) and (22S,23S)-3β-bromo-5,22,23-trihydroxystigmastan-6-one (compound 2), inhibit herpes simplex virus type 1 (HSV-1) replication and spreading in human epithelial cells derived from ocular tissues. Both compounds reduce the incidence and severity of lesions in a murine model of herpetic stromal keratitis when administered in different treatment modalities. Since encephalitis caused by HSV-1 is another immunopathology of viral origin, we evaluate here the antiviral effect of both compounds on HSV-1 infected nervous cell lines as well as their anti-inflammatory action. We found that both stigmasterol derivatives presented low cytotoxicity in the three nervous cell lines assayed. Regarding the antiviral activity, in all cases both compounds prevented HSV-1 multiplication when added after infection, as well as virus propagation. Additionally, both compounds were able to hinder interleukin-6 and Interferon-gamma secretion induced by HSV-1 infection in Neuro-2a cells. We conclude that compounds 1 and 2 have exerted a dual antiviral and anti-inflammatory effect in HSV-1 infected nervous cell lines, which makes them interesting molecules to be further studied. © 2014 Erina Petrera et al. Fil:Petrera, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Níttolo, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alché, L.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_23146133_v2014_n_p_Petrera http://hdl.handle.net/20.500.12110/paper_23146133_v2014_n_p_Petrera
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one gamma interferon interleukin 6 stigmasterol unclassified drug 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one antiinflammatory agent antivirus agent cholestane derivative gamma interferon interleukin 6 stigmasterol animal cell antiinflammatory activity antiviral activity Article controlled study cytokine production cytotoxicity assay herpes simplex keratitis Herpes simplex virus 1 human human cell immunopathology interferon production nerve cell nonhuman virus inhibition virus replication analogs and derivatives animal cell line Chlorocebus aethiops drug effects epithelium cell Herpes simplex virus 1 inflammation metabolism mouse Vero cell line virus replication Human herpesvirus 1 Murinae Simplexvirus Animals Anti-Inflammatory Agents Antiviral Agents Cell Line Cercopithecus aethiops Cholestanones Epithelial Cells Herpesvirus 1, Human Humans Inflammation Interferon-gamma Interleukin-6 Mice Stigmasterol Vero Cells Virus Replication
spellingShingle	22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one gamma interferon interleukin 6 stigmasterol unclassified drug 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one antiinflammatory agent antivirus agent cholestane derivative gamma interferon interleukin 6 stigmasterol animal cell antiinflammatory activity antiviral activity Article controlled study cytokine production cytotoxicity assay herpes simplex keratitis Herpes simplex virus 1 human human cell immunopathology interferon production nerve cell nonhuman virus inhibition virus replication analogs and derivatives animal cell line Chlorocebus aethiops drug effects epithelium cell Herpes simplex virus 1 inflammation metabolism mouse Vero cell line virus replication Human herpesvirus 1 Murinae Simplexvirus Animals Anti-Inflammatory Agents Antiviral Agents Cell Line Cercopithecus aethiops Cholestanones Epithelial Cells Herpesvirus 1, Human Humans Inflammation Interferon-gamma Interleukin-6 Mice Stigmasterol Vero Cells Virus Replication Petrera, Erina Níttolo, Analía Gabriela Alche, Laura Edith Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro
topic_facet	22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one gamma interferon interleukin 6 stigmasterol unclassified drug 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one antiinflammatory agent antivirus agent cholestane derivative gamma interferon interleukin 6 stigmasterol animal cell antiinflammatory activity antiviral activity Article controlled study cytokine production cytotoxicity assay herpes simplex keratitis Herpes simplex virus 1 human human cell immunopathology interferon production nerve cell nonhuman virus inhibition virus replication analogs and derivatives animal cell line Chlorocebus aethiops drug effects epithelium cell Herpes simplex virus 1 inflammation metabolism mouse Vero cell line virus replication Human herpesvirus 1 Murinae Simplexvirus Animals Anti-Inflammatory Agents Antiviral Agents Cell Line Cercopithecus aethiops Cholestanones Epithelial Cells Herpesvirus 1, Human Humans Inflammation Interferon-gamma Interleukin-6 Mice Stigmasterol Vero Cells Virus Replication
description	Polyfunctionalized stigmasterol derivatives, (22S,23S)-22,23-dihydroxystigmast-4-en-3-one (compound 1) and (22S,23S)-3β-bromo-5,22,23-trihydroxystigmastan-6-one (compound 2), inhibit herpes simplex virus type 1 (HSV-1) replication and spreading in human epithelial cells derived from ocular tissues. Both compounds reduce the incidence and severity of lesions in a murine model of herpetic stromal keratitis when administered in different treatment modalities. Since encephalitis caused by HSV-1 is another immunopathology of viral origin, we evaluate here the antiviral effect of both compounds on HSV-1 infected nervous cell lines as well as their anti-inflammatory action. We found that both stigmasterol derivatives presented low cytotoxicity in the three nervous cell lines assayed. Regarding the antiviral activity, in all cases both compounds prevented HSV-1 multiplication when added after infection, as well as virus propagation. Additionally, both compounds were able to hinder interleukin-6 and Interferon-gamma secretion induced by HSV-1 infection in Neuro-2a cells. We conclude that compounds 1 and 2 have exerted a dual antiviral and anti-inflammatory effect in HSV-1 infected nervous cell lines, which makes them interesting molecules to be further studied. © 2014 Erina Petrera et al.
author	Petrera, Erina Níttolo, Analía Gabriela Alche, Laura Edith
author_facet	Petrera, Erina Níttolo, Analía Gabriela Alche, Laura Edith
author_sort	Petrera, Erina
title	Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro
title_short	Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro
title_full	Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro
title_fullStr	Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro
title_full_unstemmed	Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro
title_sort	antiviral action of synthetic stigmasterol derivatives on herpes simplex virus replication in nervous cells in vitro
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_23146133_v2014_n_p_Petrera http://hdl.handle.net/20.500.12110/paper_23146133_v2014_n_p_Petrera
work_keys_str_mv	AT petreraerina antiviralactionofsyntheticstigmasterolderivativesonherpessimplexvirusreplicationinnervouscellsinvitro AT nittoloanaliagabriela antiviralactionofsyntheticstigmasterolderivativesonherpessimplexvirusreplicationinnervouscellsinvitro AT alchelauraedith antiviralactionofsyntheticstigmasterolderivativesonherpessimplexvirusreplicationinnervouscellsinvitro
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Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro

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