Simultaneous production assessment of triacylglycerols for biodiesel and exopolysaccharides as valuable co-products in Navicula cincta

The marine benthic diatom Navicula cincta was cultured in order to evaluate its capacity to produce both neutral lipid-triacylglycerols (TAG) for biodiesel, and exopolysaccharides (EPS), as valuable co-products, under the same environmental conditions. The species was cultured without applying stres...

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Autores principales: Rodriguez, María Cecilia, Matulewicz, María Cristina
Publicado: 2016
Materias:
Biodiesel
Diatoms
Exopolysaccharides
Navicula cincta
Triacylglycerols
Valuable co-products
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_22119264_v15_n_p120_Bielsa
http://hdl.handle.net/20.500.12110/paper_22119264_v15_n_p120_Bielsa
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_22119264_v15_n_p120_Bielsa
record_format dspace
spelling paper:paper_22119264_v15_n_p120_Bielsa2023-06-08T16:35:14Z Simultaneous production assessment of triacylglycerols for biodiesel and exopolysaccharides as valuable co-products in Navicula cincta Rodriguez, María Cecilia Matulewicz, María Cristina Biodiesel Diatoms Exopolysaccharides Navicula cincta Triacylglycerols Valuable co-products The marine benthic diatom Navicula cincta was cultured in order to evaluate its capacity to produce both neutral lipid-triacylglycerols (TAG) for biodiesel, and exopolysaccharides (EPS), as valuable co-products, under the same environmental conditions. The species was cultured without applying stress conditions, except for the ones naturally created by the culture age. Peaks of neutral lipid accumulation were estimated by fluorimetry by Nile Red. Consequently, lipids were extracted and fractionated into neutral and polar fractions and the fatty acid profile of each fraction analysed by GC. The stationary phase began on day 6, when phosphate and silicate reached limiting values for diatom growth. Total lipids and lipid fractions did not show differences between harvesting time points, reaching total lipid up to 41% of ash-free dry weight (AFDW) and TAG the dominant fraction (ca. 90% of total lipids). Particularly noticeable was the storage of palmitoleic acid (ca. 54% of total fatty acid methyl esters) and a lower level of polyunsaturated fatty acids, which may impart overall favourable properties to a biodiesel fuel, especially cold flow and oxidative stability. The maximum concentrations of EPS corresponded to soluble fraction, which was most significant when the cultures reached the stationary phase and when the medium was almost phosphate and silicate depleted. The characterization of soluble EPS indicated the presence of N-glycopeptides. According to the present results, a hypothetical scheme of demonstrative cultures under a biorefinery approach is proposed for N. cincta. © 2016 Elsevier B.V. Fil:Rodríguez, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matulewicz, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_22119264_v15_n_p120_Bielsa http://hdl.handle.net/20.500.12110/paper_22119264_v15_n_p120_Bielsa
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Biodiesel
Diatoms
Exopolysaccharides
Navicula cincta
Triacylglycerols
Valuable co-products
spellingShingle Biodiesel
Diatoms
Exopolysaccharides
Navicula cincta
Triacylglycerols
Valuable co-products
Rodriguez, María Cecilia
Matulewicz, María Cristina
Simultaneous production assessment of triacylglycerols for biodiesel and exopolysaccharides as valuable co-products in Navicula cincta
topic_facet Biodiesel
Diatoms
Exopolysaccharides
Navicula cincta
Triacylglycerols
Valuable co-products
description The marine benthic diatom Navicula cincta was cultured in order to evaluate its capacity to produce both neutral lipid-triacylglycerols (TAG) for biodiesel, and exopolysaccharides (EPS), as valuable co-products, under the same environmental conditions. The species was cultured without applying stress conditions, except for the ones naturally created by the culture age. Peaks of neutral lipid accumulation were estimated by fluorimetry by Nile Red. Consequently, lipids were extracted and fractionated into neutral and polar fractions and the fatty acid profile of each fraction analysed by GC. The stationary phase began on day 6, when phosphate and silicate reached limiting values for diatom growth. Total lipids and lipid fractions did not show differences between harvesting time points, reaching total lipid up to 41% of ash-free dry weight (AFDW) and TAG the dominant fraction (ca. 90% of total lipids). Particularly noticeable was the storage of palmitoleic acid (ca. 54% of total fatty acid methyl esters) and a lower level of polyunsaturated fatty acids, which may impart overall favourable properties to a biodiesel fuel, especially cold flow and oxidative stability. The maximum concentrations of EPS corresponded to soluble fraction, which was most significant when the cultures reached the stationary phase and when the medium was almost phosphate and silicate depleted. The characterization of soluble EPS indicated the presence of N-glycopeptides. According to the present results, a hypothetical scheme of demonstrative cultures under a biorefinery approach is proposed for N. cincta. © 2016 Elsevier B.V.
author Rodriguez, María Cecilia
Matulewicz, María Cristina
author_facet Rodriguez, María Cecilia
Matulewicz, María Cristina
author_sort Rodriguez, María Cecilia
title Simultaneous production assessment of triacylglycerols for biodiesel and exopolysaccharides as valuable co-products in Navicula cincta
title_short Simultaneous production assessment of triacylglycerols for biodiesel and exopolysaccharides as valuable co-products in Navicula cincta
title_full Simultaneous production assessment of triacylglycerols for biodiesel and exopolysaccharides as valuable co-products in Navicula cincta
title_fullStr Simultaneous production assessment of triacylglycerols for biodiesel and exopolysaccharides as valuable co-products in Navicula cincta
title_full_unstemmed Simultaneous production assessment of triacylglycerols for biodiesel and exopolysaccharides as valuable co-products in Navicula cincta
title_sort simultaneous production assessment of triacylglycerols for biodiesel and exopolysaccharides as valuable co-products in navicula cincta
publishDate 2016
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_22119264_v15_n_p120_Bielsa
http://hdl.handle.net/20.500.12110/paper_22119264_v15_n_p120_Bielsa
work_keys_str_mv AT rodriguezmariacecilia simultaneousproductionassessmentoftriacylglycerolsforbiodieselandexopolysaccharidesasvaluablecoproductsinnaviculacincta
AT matulewiczmariacristina simultaneousproductionassessmentoftriacylglycerolsforbiodieselandexopolysaccharidesasvaluablecoproductsinnaviculacincta
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