Improvement of Chicken Primordial Germ Cell Maintenance In Vitro by Blockade of the Aryl Hydrocarbon Receptor Endogenous Activity

Primordial germ cells (PGCs) are the undifferentiated progenitors of gametes. Germline competent PGCs can be developed as a cell-based system for genetic modification in chickens, which provides a valuable tool for transgenic technology with both research and industrial applications. This implies ma...

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Autores principales:	Barañao, José Lino S., Bussmann, Ursula A.
Publicado:	2016
Materias:	alkaline phosphatase alpha naphthoflavone aromatic hydrocarbon receptor stage specific embryo antigen 1 alpha-naphthoflavone benzoflavone derivative animal experiment Article chicken controlled study culture medium embryo flow cytometry hematopoietic stem cell immunofluorescence in vitro study nonhuman primordial germ cell priority journal stable transfection animal antagonists and inhibitors cell culture cytology drug effects female germ cell signal transduction Animals Benzoflavones Cells, Cultured Chickens Female Germ Cells Receptors, Aryl Hydrocarbon Signal Transduction
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_21524971_v18_n3_p154_PerezSaez http://hdl.handle.net/20.500.12110/paper_21524971_v18_n3_p154_PerezSaez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_21524971_v18_n3_p154_PerezSaez
record_format	dspace
spelling	paper:paper_21524971_v18_n3_p154_PerezSaez2023-06-08T16:34:22Z Improvement of Chicken Primordial Germ Cell Maintenance In Vitro by Blockade of the Aryl Hydrocarbon Receptor Endogenous Activity Barañao, José Lino S. Bussmann, Ursula A. alkaline phosphatase alpha naphthoflavone aromatic hydrocarbon receptor stage specific embryo antigen 1 alpha-naphthoflavone aromatic hydrocarbon receptor benzoflavone derivative animal experiment Article chicken controlled study culture medium embryo flow cytometry hematopoietic stem cell immunofluorescence in vitro study nonhuman primordial germ cell priority journal stable transfection animal antagonists and inhibitors cell culture chicken cytology drug effects female germ cell signal transduction Animals Benzoflavones Cells, Cultured Chickens Female Germ Cells Receptors, Aryl Hydrocarbon Signal Transduction Primordial germ cells (PGCs) are the undifferentiated progenitors of gametes. Germline competent PGCs can be developed as a cell-based system for genetic modification in chickens, which provides a valuable tool for transgenic technology with both research and industrial applications. This implies manipulation of PGCs, which, in recent years, encouraged a lot of research focused on the study of PGCs and the way of improving their culture. The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that besides mediating toxic responses to environmental contaminants plays pivotal physiological roles in various biological processes. Since a novel compound that acts as an antagonist of this receptor has been reported to promote expansion of hematopoietic stem cells, we conducted the present study with the aim of determining whether addition of an established AHR antagonist to the standard culture medium used nowadays for in vitro chicken PGCs culture improves ex vivo expansion. We have found that addition of α-naphthoflavone in culture medium promotes the amplification of undifferentiated cells and that this effect is exerted by the blockade of AHR action. Our results constitute the first report of the successful use of a readily available AHR antagonist to improve avian PGCs expansion, and they further extend the knowledge of the effects of AHR modulation in undifferentiated cells. Copyright © 2016 Mary Ann Liebert, Inc. Fil:Barañao, J.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bussmann, U.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_21524971_v18_n3_p154_PerezSaez http://hdl.handle.net/20.500.12110/paper_21524971_v18_n3_p154_PerezSaez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	alkaline phosphatase alpha naphthoflavone aromatic hydrocarbon receptor stage specific embryo antigen 1 alpha-naphthoflavone aromatic hydrocarbon receptor benzoflavone derivative animal experiment Article chicken controlled study culture medium embryo flow cytometry hematopoietic stem cell immunofluorescence in vitro study nonhuman primordial germ cell priority journal stable transfection animal antagonists and inhibitors cell culture chicken cytology drug effects female germ cell signal transduction Animals Benzoflavones Cells, Cultured Chickens Female Germ Cells Receptors, Aryl Hydrocarbon Signal Transduction
spellingShingle	alkaline phosphatase alpha naphthoflavone aromatic hydrocarbon receptor stage specific embryo antigen 1 alpha-naphthoflavone aromatic hydrocarbon receptor benzoflavone derivative animal experiment Article chicken controlled study culture medium embryo flow cytometry hematopoietic stem cell immunofluorescence in vitro study nonhuman primordial germ cell priority journal stable transfection animal antagonists and inhibitors cell culture chicken cytology drug effects female germ cell signal transduction Animals Benzoflavones Cells, Cultured Chickens Female Germ Cells Receptors, Aryl Hydrocarbon Signal Transduction Barañao, José Lino S. Bussmann, Ursula A. Improvement of Chicken Primordial Germ Cell Maintenance In Vitro by Blockade of the Aryl Hydrocarbon Receptor Endogenous Activity
topic_facet	alkaline phosphatase alpha naphthoflavone aromatic hydrocarbon receptor stage specific embryo antigen 1 alpha-naphthoflavone aromatic hydrocarbon receptor benzoflavone derivative animal experiment Article chicken controlled study culture medium embryo flow cytometry hematopoietic stem cell immunofluorescence in vitro study nonhuman primordial germ cell priority journal stable transfection animal antagonists and inhibitors cell culture chicken cytology drug effects female germ cell signal transduction Animals Benzoflavones Cells, Cultured Chickens Female Germ Cells Receptors, Aryl Hydrocarbon Signal Transduction
description	Primordial germ cells (PGCs) are the undifferentiated progenitors of gametes. Germline competent PGCs can be developed as a cell-based system for genetic modification in chickens, which provides a valuable tool for transgenic technology with both research and industrial applications. This implies manipulation of PGCs, which, in recent years, encouraged a lot of research focused on the study of PGCs and the way of improving their culture. The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that besides mediating toxic responses to environmental contaminants plays pivotal physiological roles in various biological processes. Since a novel compound that acts as an antagonist of this receptor has been reported to promote expansion of hematopoietic stem cells, we conducted the present study with the aim of determining whether addition of an established AHR antagonist to the standard culture medium used nowadays for in vitro chicken PGCs culture improves ex vivo expansion. We have found that addition of α-naphthoflavone in culture medium promotes the amplification of undifferentiated cells and that this effect is exerted by the blockade of AHR action. Our results constitute the first report of the successful use of a readily available AHR antagonist to improve avian PGCs expansion, and they further extend the knowledge of the effects of AHR modulation in undifferentiated cells. Copyright © 2016 Mary Ann Liebert, Inc.
author	Barañao, José Lino S. Bussmann, Ursula A.
author_facet	Barañao, José Lino S. Bussmann, Ursula A.
author_sort	Barañao, José Lino S.
title	Improvement of Chicken Primordial Germ Cell Maintenance In Vitro by Blockade of the Aryl Hydrocarbon Receptor Endogenous Activity
title_short	Improvement of Chicken Primordial Germ Cell Maintenance In Vitro by Blockade of the Aryl Hydrocarbon Receptor Endogenous Activity
title_full	Improvement of Chicken Primordial Germ Cell Maintenance In Vitro by Blockade of the Aryl Hydrocarbon Receptor Endogenous Activity
title_fullStr	Improvement of Chicken Primordial Germ Cell Maintenance In Vitro by Blockade of the Aryl Hydrocarbon Receptor Endogenous Activity
title_full_unstemmed	Improvement of Chicken Primordial Germ Cell Maintenance In Vitro by Blockade of the Aryl Hydrocarbon Receptor Endogenous Activity
title_sort	improvement of chicken primordial germ cell maintenance in vitro by blockade of the aryl hydrocarbon receptor endogenous activity
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_21524971_v18_n3_p154_PerezSaez http://hdl.handle.net/20.500.12110/paper_21524971_v18_n3_p154_PerezSaez
work_keys_str_mv	AT baranaojoselinos improvementofchickenprimordialgermcellmaintenanceinvitrobyblockadeofthearylhydrocarbonreceptorendogenousactivity AT bussmannursulaa improvementofchickenprimordialgermcellmaintenanceinvitrobyblockadeofthearylhydrocarbonreceptorendogenousactivity
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Improvement of Chicken Primordial Germ Cell Maintenance In Vitro by Blockade of the Aryl Hydrocarbon Receptor Endogenous Activity

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