Proteins as nano-carriers for bioactive compounds. The case of 7S and 11S soy globulins and folic acid complexation

Isolated 7S and 11S globulins obtained from defeated soy flour were complexated with folic acid (FA) in order to generate nano-carriers for this important vitamin in human nutrition. Fluorescence spectroscopy and dynamic light scattering were applied to follow the nano-complexes formation and for th...

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Publicado:	2018
Materias:	7S and 11S globulins Folic acid Lactobacillus casei BL23 Nano-complexation Binding sites Bins Bioactivity Fluorescence Fluorescence spectroscopy Light scattering Organic acids Particle size Particle size analysis 11S globulin Bioactive compounds Double logarithms Folic acids Lactobacillus casei Peptide receptor Protein aggregation Protein molecules Proteins
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20734360_v10_n2_p_Ochnio http://hdl.handle.net/20.500.12110/paper_20734360_v10_n2_p_Ochnio
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_20734360_v10_n2_p_Ochnio
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spelling	paper:paper_20734360_v10_n2_p_Ochnio2023-06-08T16:34:11Z Proteins as nano-carriers for bioactive compounds. The case of 7S and 11S soy globulins and folic acid complexation 7S and 11S globulins Folic acid Lactobacillus casei BL23 Nano-complexation Binding sites Bins Bioactivity Fluorescence Fluorescence spectroscopy Light scattering Organic acids Particle size Particle size analysis 11S globulin Bioactive compounds Double logarithms Folic acids Lactobacillus casei Peptide receptor Protein aggregation Protein molecules Proteins Isolated 7S and 11S globulins obtained from defeated soy flour were complexated with folic acid (FA) in order to generate nano-carriers for this important vitamin in human nutrition. Fluorescence spectroscopy and dynamic light scattering were applied to follow the nano-complexes formation and for their characterization. Fluorescence experimental data were modeled by the Stern-Volmer and a modified double logarithm approach. The results obtained confirmed static quenching. The number of binding sites on the protein molecule was ~1. The values obtained for the binding constants suggest a high affinity between proteins and FA. Particle size distribution allowed to study the protein aggregation phenomenon induced by FA bound to the native proteins. Z-average manifested a clear trend to protein aggregation. 11S-FA nano-complexes resulted in more polydispersity. ζ-potential of FA nano-complexes did not show a remarkable change after FA complexation. The biological activity of nano-complexes loaded with FA was explored in terms of their capacity to enhance the biomass formation of Lactobacillus casei BL23. The results concerning to nano-complexes inclusion in culture media showed higher bacterial growth. Such a result was attributed to the entry of the acid by the specific receptors concomitantly by the peptide receptors. These findings have technological impact for the use of globulins-FA based nano-complexes in nutraceutical, pharmaceutical and food industries. © 2018 by the authors. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20734360_v10_n2_p_Ochnio http://hdl.handle.net/20.500.12110/paper_20734360_v10_n2_p_Ochnio
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	7S and 11S globulins Folic acid Lactobacillus casei BL23 Nano-complexation Binding sites Bins Bioactivity Fluorescence Fluorescence spectroscopy Light scattering Organic acids Particle size Particle size analysis 11S globulin Bioactive compounds Double logarithms Folic acids Lactobacillus casei Peptide receptor Protein aggregation Protein molecules Proteins
spellingShingle	7S and 11S globulins Folic acid Lactobacillus casei BL23 Nano-complexation Binding sites Bins Bioactivity Fluorescence Fluorescence spectroscopy Light scattering Organic acids Particle size Particle size analysis 11S globulin Bioactive compounds Double logarithms Folic acids Lactobacillus casei Peptide receptor Protein aggregation Protein molecules Proteins Proteins as nano-carriers for bioactive compounds. The case of 7S and 11S soy globulins and folic acid complexation
topic_facet	7S and 11S globulins Folic acid Lactobacillus casei BL23 Nano-complexation Binding sites Bins Bioactivity Fluorescence Fluorescence spectroscopy Light scattering Organic acids Particle size Particle size analysis 11S globulin Bioactive compounds Double logarithms Folic acids Lactobacillus casei Peptide receptor Protein aggregation Protein molecules Proteins
description	Isolated 7S and 11S globulins obtained from defeated soy flour were complexated with folic acid (FA) in order to generate nano-carriers for this important vitamin in human nutrition. Fluorescence spectroscopy and dynamic light scattering were applied to follow the nano-complexes formation and for their characterization. Fluorescence experimental data were modeled by the Stern-Volmer and a modified double logarithm approach. The results obtained confirmed static quenching. The number of binding sites on the protein molecule was ~1. The values obtained for the binding constants suggest a high affinity between proteins and FA. Particle size distribution allowed to study the protein aggregation phenomenon induced by FA bound to the native proteins. Z-average manifested a clear trend to protein aggregation. 11S-FA nano-complexes resulted in more polydispersity. ζ-potential of FA nano-complexes did not show a remarkable change after FA complexation. The biological activity of nano-complexes loaded with FA was explored in terms of their capacity to enhance the biomass formation of Lactobacillus casei BL23. The results concerning to nano-complexes inclusion in culture media showed higher bacterial growth. Such a result was attributed to the entry of the acid by the specific receptors concomitantly by the peptide receptors. These findings have technological impact for the use of globulins-FA based nano-complexes in nutraceutical, pharmaceutical and food industries. © 2018 by the authors.
title	Proteins as nano-carriers for bioactive compounds. The case of 7S and 11S soy globulins and folic acid complexation
title_short	Proteins as nano-carriers for bioactive compounds. The case of 7S and 11S soy globulins and folic acid complexation
title_full	Proteins as nano-carriers for bioactive compounds. The case of 7S and 11S soy globulins and folic acid complexation
title_fullStr	Proteins as nano-carriers for bioactive compounds. The case of 7S and 11S soy globulins and folic acid complexation
title_full_unstemmed	Proteins as nano-carriers for bioactive compounds. The case of 7S and 11S soy globulins and folic acid complexation
title_sort	proteins as nano-carriers for bioactive compounds. the case of 7s and 11s soy globulins and folic acid complexation
publishDate	2018
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20734360_v10_n2_p_Ochnio http://hdl.handle.net/20.500.12110/paper_20734360_v10_n2_p_Ochnio
_version_	1768546465678884864

Proteins as nano-carriers for bioactive compounds. The case of 7S and 11S soy globulins and folic acid complexation

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