A three-domain copper-nitrite reductase with a unique sensing loop
Dissimilatory nitrite reductases are key enzymes in the denitrification pathway, reducing nitrite and leading to the production of gaseous products (NO, N2O and N2). The reaction is catalysed either by a Cu-containing nitrite reductase (NirK) or by a cytochrome cd 1 nitrite reductase (NirS), as the...
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2019
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20522525_v6_n_p248_Opperman http://hdl.handle.net/20.500.12110/paper_20522525_v6_n_p248_Opperman |
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paper:paper_20522525_v6_n_p248_Opperman2023-06-08T16:33:58Z A three-domain copper-nitrite reductase with a unique sensing loop Ser Thermus scotoductus SA-01 three-domain copper-nitrite reductase X-ray crystal structure Dissimilatory nitrite reductases are key enzymes in the denitrification pathway, reducing nitrite and leading to the production of gaseous products (NO, N2O and N2). The reaction is catalysed either by a Cu-containing nitrite reductase (NirK) or by a cytochrome cd 1 nitrite reductase (NirS), as the simultaneous presence of the two enzymes has never been detected in the same microorganism. The thermophilic bacterium Thermus scotoductus SA-01 is an exception to this rule, harbouring both genes within a denitrification cluster, which encodes for an atypical NirK. The crystal structure of TsNirK has been determined at 1.63Å resolution. TsNirK is a homotrimer with subunits of 451 residues that contain three copper atoms each. The N-Terminal region possesses a type 2 Cu (T2Cu) and a type 1 Cu (T1CuN) while the C-Terminus contains an extra type 1 Cu (T1CuC) bound within a cupredoxin motif. T1CuN shows an unusual Cu atom coordination (His2-Cys-Gln) compared with T1Cu observed in NirKs reported so far (His2-Cys-Met). T1CuC is buried at ∼5Å from the molecular surface and located ∼14.1Å away from T1CuN; T1CuN and T2Cu are ∼12.6Å apart. All these distances are compatible with an electron-Transfer process T1CuC → T1CuN → T2Cu. T1CuN and T2Cu are connected by a typical Cys-His bridge and an unexpected sensing loop which harbours a SerCAT residue close to T2Cu, suggesting an alternative nitrite-reduction mechanism in these enzymes. Biophysicochemical and functional features of TsNirK are discussed on the basis of X-ray crystallography, electron paramagnetic resonance, resonance Raman and kinetic experiments. © 2019. 2019 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20522525_v6_n_p248_Opperman http://hdl.handle.net/20.500.12110/paper_20522525_v6_n_p248_Opperman |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Ser Thermus scotoductus SA-01 three-domain copper-nitrite reductase X-ray crystal structure |
| spellingShingle |
Ser Thermus scotoductus SA-01 three-domain copper-nitrite reductase X-ray crystal structure A three-domain copper-nitrite reductase with a unique sensing loop |
| topic_facet |
Ser Thermus scotoductus SA-01 three-domain copper-nitrite reductase X-ray crystal structure |
| description |
Dissimilatory nitrite reductases are key enzymes in the denitrification pathway, reducing nitrite and leading to the production of gaseous products (NO, N2O and N2). The reaction is catalysed either by a Cu-containing nitrite reductase (NirK) or by a cytochrome cd 1 nitrite reductase (NirS), as the simultaneous presence of the two enzymes has never been detected in the same microorganism. The thermophilic bacterium Thermus scotoductus SA-01 is an exception to this rule, harbouring both genes within a denitrification cluster, which encodes for an atypical NirK. The crystal structure of TsNirK has been determined at 1.63Å resolution. TsNirK is a homotrimer with subunits of 451 residues that contain three copper atoms each. The N-Terminal region possesses a type 2 Cu (T2Cu) and a type 1 Cu (T1CuN) while the C-Terminus contains an extra type 1 Cu (T1CuC) bound within a cupredoxin motif. T1CuN shows an unusual Cu atom coordination (His2-Cys-Gln) compared with T1Cu observed in NirKs reported so far (His2-Cys-Met). T1CuC is buried at ∼5Å from the molecular surface and located ∼14.1Å away from T1CuN; T1CuN and T2Cu are ∼12.6Å apart. All these distances are compatible with an electron-Transfer process T1CuC → T1CuN → T2Cu. T1CuN and T2Cu are connected by a typical Cys-His bridge and an unexpected sensing loop which harbours a SerCAT residue close to T2Cu, suggesting an alternative nitrite-reduction mechanism in these enzymes. Biophysicochemical and functional features of TsNirK are discussed on the basis of X-ray crystallography, electron paramagnetic resonance, resonance Raman and kinetic experiments. © 2019. |
| title |
A three-domain copper-nitrite reductase with a unique sensing loop |
| title_short |
A three-domain copper-nitrite reductase with a unique sensing loop |
| title_full |
A three-domain copper-nitrite reductase with a unique sensing loop |
| title_fullStr |
A three-domain copper-nitrite reductase with a unique sensing loop |
| title_full_unstemmed |
A three-domain copper-nitrite reductase with a unique sensing loop |
| title_sort |
three-domain copper-nitrite reductase with a unique sensing loop |
| publishDate |
2019 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20522525_v6_n_p248_Opperman http://hdl.handle.net/20.500.12110/paper_20522525_v6_n_p248_Opperman |
| _version_ |
1768542761484550144 |