Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock
In vertebrate development, the sequential and rhythmic segmentation of the body axis is regulated by a "segmentation clock". This clock is comprised of a population of coordinated oscillating cells that together produce rhythmic gene expression patterns in the embryo. Whether individual ce...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_2050084X_v5_nFEBRUARY2016_p_Webb http://hdl.handle.net/20.500.12110/paper_2050084X_v5_nFEBRUARY2016_p_Webb |
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paper:paper_2050084X_v5_nFEBRUARY2016_p_Webb2023-06-08T16:33:50Z Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock animal cell Article circadian rhythm embryo segmentation gene expression assay immunocytochemistry nonhuman oscillation zebra fish animal biological rhythm biosynthesis cell culture cell function embryology gene expression profiling gene fusion physiology reporter gene transgenic animal basic helix loop helix transcription factor her1 protein, zebrafish zebrafish protein Animals Animals, Genetically Modified Artificial Gene Fusion Basic Helix-Loop-Helix Transcription Factors Biological Clocks Cell Physiological Phenomena Cells, Cultured Gene Expression Profiling Genes, Reporter Zebrafish Zebrafish Proteins In vertebrate development, the sequential and rhythmic segmentation of the body axis is regulated by a "segmentation clock". This clock is comprised of a population of coordinated oscillating cells that together produce rhythmic gene expression patterns in the embryo. Whether individual cells autonomously maintain oscillations, or whether oscillations depend on signals from neighboring cells is unknown. Using a transgenic zebrafish reporter line for the cyclic transcription factor Her1, we recorded single tailbud cells in vitro. We demonstrate that individual cells can behave as autonomous cellular oscillators. We described the observed variability in cell behavior using a theory of generic oscillators with correlated noise. Single cells have longer periods and lower precision than the tissue, highlighting the role of collective processes in the segmentation clock. Our work reveals a population of cells from the zebrafish segmentation clock that behave as self-sustained, autonomous oscillators with distinctive noisy dynamics. © Webb et al. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_2050084X_v5_nFEBRUARY2016_p_Webb http://hdl.handle.net/20.500.12110/paper_2050084X_v5_nFEBRUARY2016_p_Webb |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
animal cell Article circadian rhythm embryo segmentation gene expression assay immunocytochemistry nonhuman oscillation zebra fish animal biological rhythm biosynthesis cell culture cell function embryology gene expression profiling gene fusion physiology reporter gene transgenic animal basic helix loop helix transcription factor her1 protein, zebrafish zebrafish protein Animals Animals, Genetically Modified Artificial Gene Fusion Basic Helix-Loop-Helix Transcription Factors Biological Clocks Cell Physiological Phenomena Cells, Cultured Gene Expression Profiling Genes, Reporter Zebrafish Zebrafish Proteins |
| spellingShingle |
animal cell Article circadian rhythm embryo segmentation gene expression assay immunocytochemistry nonhuman oscillation zebra fish animal biological rhythm biosynthesis cell culture cell function embryology gene expression profiling gene fusion physiology reporter gene transgenic animal basic helix loop helix transcription factor her1 protein, zebrafish zebrafish protein Animals Animals, Genetically Modified Artificial Gene Fusion Basic Helix-Loop-Helix Transcription Factors Biological Clocks Cell Physiological Phenomena Cells, Cultured Gene Expression Profiling Genes, Reporter Zebrafish Zebrafish Proteins Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock |
| topic_facet |
animal cell Article circadian rhythm embryo segmentation gene expression assay immunocytochemistry nonhuman oscillation zebra fish animal biological rhythm biosynthesis cell culture cell function embryology gene expression profiling gene fusion physiology reporter gene transgenic animal basic helix loop helix transcription factor her1 protein, zebrafish zebrafish protein Animals Animals, Genetically Modified Artificial Gene Fusion Basic Helix-Loop-Helix Transcription Factors Biological Clocks Cell Physiological Phenomena Cells, Cultured Gene Expression Profiling Genes, Reporter Zebrafish Zebrafish Proteins |
| description |
In vertebrate development, the sequential and rhythmic segmentation of the body axis is regulated by a "segmentation clock". This clock is comprised of a population of coordinated oscillating cells that together produce rhythmic gene expression patterns in the embryo. Whether individual cells autonomously maintain oscillations, or whether oscillations depend on signals from neighboring cells is unknown. Using a transgenic zebrafish reporter line for the cyclic transcription factor Her1, we recorded single tailbud cells in vitro. We demonstrate that individual cells can behave as autonomous cellular oscillators. We described the observed variability in cell behavior using a theory of generic oscillators with correlated noise. Single cells have longer periods and lower precision than the tissue, highlighting the role of collective processes in the segmentation clock. Our work reveals a population of cells from the zebrafish segmentation clock that behave as self-sustained, autonomous oscillators with distinctive noisy dynamics. © Webb et al. |
| title |
Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock |
| title_short |
Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock |
| title_full |
Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock |
| title_fullStr |
Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock |
| title_full_unstemmed |
Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock |
| title_sort |
persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_2050084X_v5_nFEBRUARY2016_p_Webb http://hdl.handle.net/20.500.12110/paper_2050084X_v5_nFEBRUARY2016_p_Webb |
| _version_ |
1768542149345804288 |