Self-assembled phosphate-polyamine networks as biocompatible supramolecular platforms to modulate cell adhesion
The modulation of cell adhesion via biologically inspired materials plays a key role in the development of realistic platforms to envisage not only mechanistic descriptions of many physiological and pathological processes but also new biointerfacial designs compatible with the requirements of biomed...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20474830_v6_n8_p2230_Muzzio http://hdl.handle.net/20.500.12110/paper_20474830_v6_n8_p2230_Muzzio |
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paper:paper_20474830_v6_n8_p2230_Muzzio2023-06-08T16:33:48Z Self-assembled phosphate-polyamine networks as biocompatible supramolecular platforms to modulate cell adhesion Atomic force microscopy Biocompatibility Cell adhesion Cell culture Cell proliferation Coatings Medical applications Solutions Supramolecular chemistry Anti-adhesive activity Biologically inspired Biologically inspired material Biomedical applications Indentation experiment Interfacial architecture Pathological process Supramolecular films Cells biocompatible coated material biomimetic material phosphate polyamine biomaterial phosphate polyamine animal cell Article atomic force microscopy biomimetics C2C12 cell line cell adhesion cell interaction cell nucleus cell proliferation chemical modification contact angle controlled study cytoskeleton epithelial cell line female focal adhesion human human cell hydrophilicity hydrophobicity immunohistochemistry limit of quantitation MC3T3 cell line mouse nonhuman priority journal quartz crystal microbalance rigidity supramolecular chemistry surface property thickness topography 3T3 cell line absorption animal cell adhesion cell culture cell survival chemistry HeLa cell line kinetics macromolecule particle size synthesis wettability 3T3 Cells Absorption, Physiological Animals Biocompatible Materials Cell Adhesion Cell Proliferation Cell Survival Cells, Cultured HeLa Cells Humans Kinetics Macromolecular Substances Mice Microscopy, Atomic Force Particle Size Phosphates Polyamines Wettability The modulation of cell adhesion via biologically inspired materials plays a key role in the development of realistic platforms to envisage not only mechanistic descriptions of many physiological and pathological processes but also new biointerfacial designs compatible with the requirements of biomedical devices. In this work, we show that the cell adhesion and proliferation of three different cell lines can be easily manipulated by using a novel biologically inspired supramolecular coating generated via dip coating of the working substrates in an aqueous solution of polyallylamine in the presence of phosphate anions - a simple one-step modification procedure. Our results reveal that selective cell adhesion can be controlled by varying the deposition time of the coating. Cell proliferation experiments showed a cell type-dependent quasi-exponential growth demonstrating the nontoxic properties of the supramolecular platform. After reaching a certain surface coverage, the supramolecular films based on phosphate-polyamine networks displayed antiadhesive activity towards cells, irrespective of the cell type. However and most interestingly, these antiadherent substrates developed strong adhesive properties after thermal annealing at 37 °C for 3 days. These results were interpreted based on the changes in the coating hydrophilicity, topography and stiffness, with the latter being assessed by atomic force microscopy imaging and indentation experiments. The reported approach is simple, robust and flexible, and would offer opportunities for the development of tunable, biocompatible interfacial architectures to control cell attachment for various biomedical applications. © 2018 The Royal Society of Chemistry. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20474830_v6_n8_p2230_Muzzio http://hdl.handle.net/20.500.12110/paper_20474830_v6_n8_p2230_Muzzio |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Atomic force microscopy Biocompatibility Cell adhesion Cell culture Cell proliferation Coatings Medical applications Solutions Supramolecular chemistry Anti-adhesive activity Biologically inspired Biologically inspired material Biomedical applications Indentation experiment Interfacial architecture Pathological process Supramolecular films Cells biocompatible coated material biomimetic material phosphate polyamine biomaterial phosphate polyamine animal cell Article atomic force microscopy biomimetics C2C12 cell line cell adhesion cell interaction cell nucleus cell proliferation chemical modification contact angle controlled study cytoskeleton epithelial cell line female focal adhesion human human cell hydrophilicity hydrophobicity immunohistochemistry limit of quantitation MC3T3 cell line mouse nonhuman priority journal quartz crystal microbalance rigidity supramolecular chemistry surface property thickness topography 3T3 cell line absorption animal cell adhesion cell culture cell survival chemistry HeLa cell line kinetics macromolecule particle size synthesis wettability 3T3 Cells Absorption, Physiological Animals Biocompatible Materials Cell Adhesion Cell Proliferation Cell Survival Cells, Cultured HeLa Cells Humans Kinetics Macromolecular Substances Mice Microscopy, Atomic Force Particle Size Phosphates Polyamines Wettability |
| spellingShingle |
Atomic force microscopy Biocompatibility Cell adhesion Cell culture Cell proliferation Coatings Medical applications Solutions Supramolecular chemistry Anti-adhesive activity Biologically inspired Biologically inspired material Biomedical applications Indentation experiment Interfacial architecture Pathological process Supramolecular films Cells biocompatible coated material biomimetic material phosphate polyamine biomaterial phosphate polyamine animal cell Article atomic force microscopy biomimetics C2C12 cell line cell adhesion cell interaction cell nucleus cell proliferation chemical modification contact angle controlled study cytoskeleton epithelial cell line female focal adhesion human human cell hydrophilicity hydrophobicity immunohistochemistry limit of quantitation MC3T3 cell line mouse nonhuman priority journal quartz crystal microbalance rigidity supramolecular chemistry surface property thickness topography 3T3 cell line absorption animal cell adhesion cell culture cell survival chemistry HeLa cell line kinetics macromolecule particle size synthesis wettability 3T3 Cells Absorption, Physiological Animals Biocompatible Materials Cell Adhesion Cell Proliferation Cell Survival Cells, Cultured HeLa Cells Humans Kinetics Macromolecular Substances Mice Microscopy, Atomic Force Particle Size Phosphates Polyamines Wettability Self-assembled phosphate-polyamine networks as biocompatible supramolecular platforms to modulate cell adhesion |
| topic_facet |
Atomic force microscopy Biocompatibility Cell adhesion Cell culture Cell proliferation Coatings Medical applications Solutions Supramolecular chemistry Anti-adhesive activity Biologically inspired Biologically inspired material Biomedical applications Indentation experiment Interfacial architecture Pathological process Supramolecular films Cells biocompatible coated material biomimetic material phosphate polyamine biomaterial phosphate polyamine animal cell Article atomic force microscopy biomimetics C2C12 cell line cell adhesion cell interaction cell nucleus cell proliferation chemical modification contact angle controlled study cytoskeleton epithelial cell line female focal adhesion human human cell hydrophilicity hydrophobicity immunohistochemistry limit of quantitation MC3T3 cell line mouse nonhuman priority journal quartz crystal microbalance rigidity supramolecular chemistry surface property thickness topography 3T3 cell line absorption animal cell adhesion cell culture cell survival chemistry HeLa cell line kinetics macromolecule particle size synthesis wettability 3T3 Cells Absorption, Physiological Animals Biocompatible Materials Cell Adhesion Cell Proliferation Cell Survival Cells, Cultured HeLa Cells Humans Kinetics Macromolecular Substances Mice Microscopy, Atomic Force Particle Size Phosphates Polyamines Wettability |
| description |
The modulation of cell adhesion via biologically inspired materials plays a key role in the development of realistic platforms to envisage not only mechanistic descriptions of many physiological and pathological processes but also new biointerfacial designs compatible with the requirements of biomedical devices. In this work, we show that the cell adhesion and proliferation of three different cell lines can be easily manipulated by using a novel biologically inspired supramolecular coating generated via dip coating of the working substrates in an aqueous solution of polyallylamine in the presence of phosphate anions - a simple one-step modification procedure. Our results reveal that selective cell adhesion can be controlled by varying the deposition time of the coating. Cell proliferation experiments showed a cell type-dependent quasi-exponential growth demonstrating the nontoxic properties of the supramolecular platform. After reaching a certain surface coverage, the supramolecular films based on phosphate-polyamine networks displayed antiadhesive activity towards cells, irrespective of the cell type. However and most interestingly, these antiadherent substrates developed strong adhesive properties after thermal annealing at 37 °C for 3 days. These results were interpreted based on the changes in the coating hydrophilicity, topography and stiffness, with the latter being assessed by atomic force microscopy imaging and indentation experiments. The reported approach is simple, robust and flexible, and would offer opportunities for the development of tunable, biocompatible interfacial architectures to control cell attachment for various biomedical applications. © 2018 The Royal Society of Chemistry. |
| title |
Self-assembled phosphate-polyamine networks as biocompatible supramolecular platforms to modulate cell adhesion |
| title_short |
Self-assembled phosphate-polyamine networks as biocompatible supramolecular platforms to modulate cell adhesion |
| title_full |
Self-assembled phosphate-polyamine networks as biocompatible supramolecular platforms to modulate cell adhesion |
| title_fullStr |
Self-assembled phosphate-polyamine networks as biocompatible supramolecular platforms to modulate cell adhesion |
| title_full_unstemmed |
Self-assembled phosphate-polyamine networks as biocompatible supramolecular platforms to modulate cell adhesion |
| title_sort |
self-assembled phosphate-polyamine networks as biocompatible supramolecular platforms to modulate cell adhesion |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20474830_v6_n8_p2230_Muzzio http://hdl.handle.net/20.500.12110/paper_20474830_v6_n8_p2230_Muzzio |
| _version_ |
1768545349027233792 |