Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions
Focal adhesions anchor contractile actin fibers with the extracellular matrix, sense the generated tension and respond to it by changing their morphology and composition. Here we ask how this mechanosensing is enabled at the protein-network level, given the modular assembly and multitasking of focal...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v8_n1_p_MalikSheriff http://hdl.handle.net/20.500.12110/paper_20452322_v8_n1_p_MalikSheriff |
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paper:paper_20452322_v8_n1_p_MalikSheriff2023-06-08T16:33:35Z Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions Rho kinase actin filament animal cell culture cytology extracellular matrix fibroblast focal adhesion image processing mechanotransduction metabolism physiology rat Actin Cytoskeleton Animals Cells, Cultured Extracellular Matrix Fibroblasts Focal Adhesions Image Processing, Computer-Assisted Mechanotransduction, Cellular Rats rho-Associated Kinases Focal adhesions anchor contractile actin fibers with the extracellular matrix, sense the generated tension and respond to it by changing their morphology and composition. Here we ask how this mechanosensing is enabled at the protein-network level, given the modular assembly and multitasking of focal adhesions. To address this, we applied a sensitive 4-color live cell imaging approach, enabling monitoring patterns of molecular changes in single focal adhesions. Co-imaging zyxin, FAK, vinculin and paxillin revealed heterogeneities in their responses to Rho-associated kinase (ROCK)-mediated perturbations of actomyosin contractility. These responses were rather weakly correlated between the proteins, reflecting diverse compositional changes in different focal adhesions. This diversity is partially attributable to the location of focal adhesions, their area, molecular content and previous contractility perturbations, suggesting that integration of multiple local cues shapes differentially focal adhesion mechano-responsiveness. Importantly, the compositional changes upon ROCK perturbations exhibited distinct paths in different focal adhesions. Moreover, the protein exhibiting the strongest response to ROCK perturbations varied among different focal adhesions. The diversity in response patterns is plausibly enabled by the modular mode of focal adhesions assembly and can provide them the needed flexibility to perform multiple tasks by combining optimally a common set of multifunctional components. © 2018 The Author(s). 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v8_n1_p_MalikSheriff http://hdl.handle.net/20.500.12110/paper_20452322_v8_n1_p_MalikSheriff |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Rho kinase actin filament animal cell culture cytology extracellular matrix fibroblast focal adhesion image processing mechanotransduction metabolism physiology rat Actin Cytoskeleton Animals Cells, Cultured Extracellular Matrix Fibroblasts Focal Adhesions Image Processing, Computer-Assisted Mechanotransduction, Cellular Rats rho-Associated Kinases |
| spellingShingle |
Rho kinase actin filament animal cell culture cytology extracellular matrix fibroblast focal adhesion image processing mechanotransduction metabolism physiology rat Actin Cytoskeleton Animals Cells, Cultured Extracellular Matrix Fibroblasts Focal Adhesions Image Processing, Computer-Assisted Mechanotransduction, Cellular Rats rho-Associated Kinases Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions |
| topic_facet |
Rho kinase actin filament animal cell culture cytology extracellular matrix fibroblast focal adhesion image processing mechanotransduction metabolism physiology rat Actin Cytoskeleton Animals Cells, Cultured Extracellular Matrix Fibroblasts Focal Adhesions Image Processing, Computer-Assisted Mechanotransduction, Cellular Rats rho-Associated Kinases |
| description |
Focal adhesions anchor contractile actin fibers with the extracellular matrix, sense the generated tension and respond to it by changing their morphology and composition. Here we ask how this mechanosensing is enabled at the protein-network level, given the modular assembly and multitasking of focal adhesions. To address this, we applied a sensitive 4-color live cell imaging approach, enabling monitoring patterns of molecular changes in single focal adhesions. Co-imaging zyxin, FAK, vinculin and paxillin revealed heterogeneities in their responses to Rho-associated kinase (ROCK)-mediated perturbations of actomyosin contractility. These responses were rather weakly correlated between the proteins, reflecting diverse compositional changes in different focal adhesions. This diversity is partially attributable to the location of focal adhesions, their area, molecular content and previous contractility perturbations, suggesting that integration of multiple local cues shapes differentially focal adhesion mechano-responsiveness. Importantly, the compositional changes upon ROCK perturbations exhibited distinct paths in different focal adhesions. Moreover, the protein exhibiting the strongest response to ROCK perturbations varied among different focal adhesions. The diversity in response patterns is plausibly enabled by the modular mode of focal adhesions assembly and can provide them the needed flexibility to perform multiple tasks by combining optimally a common set of multifunctional components. © 2018 The Author(s). |
| title |
Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions |
| title_short |
Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions |
| title_full |
Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions |
| title_fullStr |
Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions |
| title_full_unstemmed |
Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions |
| title_sort |
diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v8_n1_p_MalikSheriff http://hdl.handle.net/20.500.12110/paper_20452322_v8_n1_p_MalikSheriff |
| _version_ |
1768542289857085440 |