Selective pressure against horizontally acquired prokaryotic genes as a driving force of plastid evolution

The plastid organelle comprises a high proportion of nucleus-encoded proteins that were acquired from different prokaryotic donors via independent horizontal gene transfers following its primary endosymbiotic origin. What forces drove the targeting of these alien proteins to the plastid remains an u...

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Autores principales:	Llorente, Briardo Ernesto, Soto, Gabriela Cynthia, Flawiá, Mirtha María, Ayub, Nicolás Daniel
Publicado:	2016
Materias:	catechol oxidase signal peptide Arabidopsis bacterium cell nucleus classification cytology enzymology eukaryotic cell evolution fungus gene expression genetic selection genetics green alga horizontal gene transfer metabolism molecular model phylogeny physiology plant genome plastid prokaryotic cell protein transport symbiosis Bacteria Biological Evolution Catechol Oxidase Cell Nucleus Chlorophyta Eukaryotic Cells Fungi Gene Expression Gene Transfer, Horizontal Genome, Plant Models, Molecular Phylogeny Plastids Prokaryotic Cells Protein Sorting Signals Protein Transport Selection, Genetic Symbiosis
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v6_n_p_Llorente http://hdl.handle.net/20.500.12110/paper_20452322_v6_n_p_Llorente
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_20452322_v6_n_p_Llorente
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spelling	paper:paper_20452322_v6_n_p_Llorente2023-06-08T16:33:26Z Selective pressure against horizontally acquired prokaryotic genes as a driving force of plastid evolution Llorente, Briardo Ernesto Soto, Gabriela Cynthia Flawiá, Mirtha María Ayub, Nicolás Daniel catechol oxidase signal peptide Arabidopsis bacterium cell nucleus classification cytology enzymology eukaryotic cell evolution fungus gene expression genetic selection genetics green alga horizontal gene transfer metabolism molecular model phylogeny physiology plant genome plastid prokaryotic cell protein transport symbiosis Arabidopsis Bacteria Biological Evolution Catechol Oxidase Cell Nucleus Chlorophyta Eukaryotic Cells Fungi Gene Expression Gene Transfer, Horizontal Genome, Plant Models, Molecular Phylogeny Plastids Prokaryotic Cells Protein Sorting Signals Protein Transport Selection, Genetic Symbiosis The plastid organelle comprises a high proportion of nucleus-encoded proteins that were acquired from different prokaryotic donors via independent horizontal gene transfers following its primary endosymbiotic origin. What forces drove the targeting of these alien proteins to the plastid remains an unresolved evolutionary question. To better understand this process we screened for suitable candidate proteins to recapitulate their prokaryote-to-eukaryote transition. Here we identify the ancient horizontal transfer of a bacterial polyphenol oxidase (PPO) gene to the nuclear genome of an early land plant ancestor and infer the possible mechanism behind the plastidial localization of the encoded enzyme. Arabidopsis plants expressing PPO versions either lacking or harbouring a plastid-targeting signal allowed examining fitness consequences associated with its subcellular localization. Markedly, a deleterious effect on plant growth was highly correlated with PPO activity only when producing the non-targeted enzyme, suggesting that selection favoured the fixation of plastid-targeted protein versions. Our results reveal a possible evolutionary mechanism of how selection against heterologous genes encoding cytosolic proteins contributed in incrementing plastid proteome complexity from non-endosymbiotic gene sources, a process that may also impact mitochondrial evolution. Fil:Llorente, B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Soto, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawiá, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ayub, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v6_n_p_Llorente http://hdl.handle.net/20.500.12110/paper_20452322_v6_n_p_Llorente
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	catechol oxidase signal peptide Arabidopsis bacterium cell nucleus classification cytology enzymology eukaryotic cell evolution fungus gene expression genetic selection genetics green alga horizontal gene transfer metabolism molecular model phylogeny physiology plant genome plastid prokaryotic cell protein transport symbiosis Arabidopsis Bacteria Biological Evolution Catechol Oxidase Cell Nucleus Chlorophyta Eukaryotic Cells Fungi Gene Expression Gene Transfer, Horizontal Genome, Plant Models, Molecular Phylogeny Plastids Prokaryotic Cells Protein Sorting Signals Protein Transport Selection, Genetic Symbiosis
spellingShingle	catechol oxidase signal peptide Arabidopsis bacterium cell nucleus classification cytology enzymology eukaryotic cell evolution fungus gene expression genetic selection genetics green alga horizontal gene transfer metabolism molecular model phylogeny physiology plant genome plastid prokaryotic cell protein transport symbiosis Arabidopsis Bacteria Biological Evolution Catechol Oxidase Cell Nucleus Chlorophyta Eukaryotic Cells Fungi Gene Expression Gene Transfer, Horizontal Genome, Plant Models, Molecular Phylogeny Plastids Prokaryotic Cells Protein Sorting Signals Protein Transport Selection, Genetic Symbiosis Llorente, Briardo Ernesto Soto, Gabriela Cynthia Flawiá, Mirtha María Ayub, Nicolás Daniel Selective pressure against horizontally acquired prokaryotic genes as a driving force of plastid evolution
topic_facet	catechol oxidase signal peptide Arabidopsis bacterium cell nucleus classification cytology enzymology eukaryotic cell evolution fungus gene expression genetic selection genetics green alga horizontal gene transfer metabolism molecular model phylogeny physiology plant genome plastid prokaryotic cell protein transport symbiosis Arabidopsis Bacteria Biological Evolution Catechol Oxidase Cell Nucleus Chlorophyta Eukaryotic Cells Fungi Gene Expression Gene Transfer, Horizontal Genome, Plant Models, Molecular Phylogeny Plastids Prokaryotic Cells Protein Sorting Signals Protein Transport Selection, Genetic Symbiosis
description	The plastid organelle comprises a high proportion of nucleus-encoded proteins that were acquired from different prokaryotic donors via independent horizontal gene transfers following its primary endosymbiotic origin. What forces drove the targeting of these alien proteins to the plastid remains an unresolved evolutionary question. To better understand this process we screened for suitable candidate proteins to recapitulate their prokaryote-to-eukaryote transition. Here we identify the ancient horizontal transfer of a bacterial polyphenol oxidase (PPO) gene to the nuclear genome of an early land plant ancestor and infer the possible mechanism behind the plastidial localization of the encoded enzyme. Arabidopsis plants expressing PPO versions either lacking or harbouring a plastid-targeting signal allowed examining fitness consequences associated with its subcellular localization. Markedly, a deleterious effect on plant growth was highly correlated with PPO activity only when producing the non-targeted enzyme, suggesting that selection favoured the fixation of plastid-targeted protein versions. Our results reveal a possible evolutionary mechanism of how selection against heterologous genes encoding cytosolic proteins contributed in incrementing plastid proteome complexity from non-endosymbiotic gene sources, a process that may also impact mitochondrial evolution.
author	Llorente, Briardo Ernesto Soto, Gabriela Cynthia Flawiá, Mirtha María Ayub, Nicolás Daniel
author_facet	Llorente, Briardo Ernesto Soto, Gabriela Cynthia Flawiá, Mirtha María Ayub, Nicolás Daniel
author_sort	Llorente, Briardo Ernesto
title	Selective pressure against horizontally acquired prokaryotic genes as a driving force of plastid evolution
title_short	Selective pressure against horizontally acquired prokaryotic genes as a driving force of plastid evolution
title_full	Selective pressure against horizontally acquired prokaryotic genes as a driving force of plastid evolution
title_fullStr	Selective pressure against horizontally acquired prokaryotic genes as a driving force of plastid evolution
title_full_unstemmed	Selective pressure against horizontally acquired prokaryotic genes as a driving force of plastid evolution
title_sort	selective pressure against horizontally acquired prokaryotic genes as a driving force of plastid evolution
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v6_n_p_Llorente http://hdl.handle.net/20.500.12110/paper_20452322_v6_n_p_Llorente
work_keys_str_mv	AT llorentebriardoernesto selectivepressureagainsthorizontallyacquiredprokaryoticgenesasadrivingforceofplastidevolution AT sotogabrielacynthia selectivepressureagainsthorizontallyacquiredprokaryoticgenesasadrivingforceofplastidevolution AT flawiamirthamaria selectivepressureagainsthorizontallyacquiredprokaryoticgenesasadrivingforceofplastidevolution AT ayubnicolasdaniel selectivepressureagainsthorizontallyacquiredprokaryoticgenesasadrivingforceofplastidevolution
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Selective pressure against horizontally acquired prokaryotic genes as a driving force of plastid evolution

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