The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
Abstract: Intestinal epithelial cells serve as mechanical barriers and active components of the mucosal immune system. These cells migrate from the crypt to the tip of the villus, where different stimuli can differentially affect their survival. Here we investigated, using in vitro and in vivo strat...
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2011
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20414889_v2_n5_p_Muglia http://hdl.handle.net/20.500.12110/paper_20414889_v2_n5_p_Muglia |
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paper:paper_20414889_v2_n5_p_Muglia2023-06-08T16:33:10Z The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine Toscano, Marta Alicia Pozner, Roberto Gabriel Apoptosis Enterocytes Galectin-1 Mucosa Small bowel caspase galectin 1 galectin 1 polysaccharide animal cell animal experiment animal model apoptosis article binding site cell migration cell proliferation cell survival cell viability controlled study crypt cell glycosylation histopathology human human cell immunoblotting in vitro study in vivo study intestine cell intestine villus mitochondrial membrane potential mitochondrion mouse mutant nonhuman priority journal protein analysis protein expression protein function small intestine small intestine mucosa wild type animal cell death cell survival cytology epithelium cell genetics male metabolism mouse mutant Mus Animals Cell Death Cell Proliferation Cell Survival Epithelial Cells Galectin 1 Humans Intestine, Small Male Mice Mice, Knockout Polysaccharides Abstract: Intestinal epithelial cells serve as mechanical barriers and active components of the mucosal immune system. These cells migrate from the crypt to the tip of the villus, where different stimuli can differentially affect their survival. Here we investigated, using in vitro and in vivo strategies, the role of galectin-1 (Gal-1), an evolutionarily conserved glycan-binding protein, in modulating the survival of human and mouse enterocytes. Both Gal-1 and its specific glyco-receptors were broadly expressed in small bowel enterocytes. Exogenous Gal-1 reduced the viability of enterocytes through apoptotic mechanisms involving activation of both caspase and mitochondrial pathways. Consistent with these findings, apoptotic cells were mainly detected at the tip of the villi, following administration of Gal-1. Moreover, Gal-1-deficient (Lgals-1-) mice showed longer villi compared with their wild-type counterparts in vivo. In an experimental model of starvation, fasted wild-type mice displayed reduced villi and lower intestinal weight compared with Lgals-1- mutant mice, an effect reflected by changes in the frequency of enterocyte apoptosis. Of note, human small bowel enterocytes were also prone to this pro-apoptotic effect. Thus, Gal-1 is broadly expressed in mucosal tissue and influences the viability of human and mouse enterocytes, an effect which might influence the migration of these cells from the crypt, the integrity of the villus and the epithelial barrier function. © 2011 Macmillan Publishers Limited All rights reserved. Fil:Toscano, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pozner, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20414889_v2_n5_p_Muglia http://hdl.handle.net/20.500.12110/paper_20414889_v2_n5_p_Muglia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Apoptosis Enterocytes Galectin-1 Mucosa Small bowel caspase galectin 1 galectin 1 polysaccharide animal cell animal experiment animal model apoptosis article binding site cell migration cell proliferation cell survival cell viability controlled study crypt cell glycosylation histopathology human human cell immunoblotting in vitro study in vivo study intestine cell intestine villus mitochondrial membrane potential mitochondrion mouse mutant nonhuman priority journal protein analysis protein expression protein function small intestine small intestine mucosa wild type animal cell death cell survival cytology epithelium cell genetics male metabolism mouse mutant Mus Animals Cell Death Cell Proliferation Cell Survival Epithelial Cells Galectin 1 Humans Intestine, Small Male Mice Mice, Knockout Polysaccharides |
| spellingShingle |
Apoptosis Enterocytes Galectin-1 Mucosa Small bowel caspase galectin 1 galectin 1 polysaccharide animal cell animal experiment animal model apoptosis article binding site cell migration cell proliferation cell survival cell viability controlled study crypt cell glycosylation histopathology human human cell immunoblotting in vitro study in vivo study intestine cell intestine villus mitochondrial membrane potential mitochondrion mouse mutant nonhuman priority journal protein analysis protein expression protein function small intestine small intestine mucosa wild type animal cell death cell survival cytology epithelium cell genetics male metabolism mouse mutant Mus Animals Cell Death Cell Proliferation Cell Survival Epithelial Cells Galectin 1 Humans Intestine, Small Male Mice Mice, Knockout Polysaccharides Toscano, Marta Alicia Pozner, Roberto Gabriel The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine |
| topic_facet |
Apoptosis Enterocytes Galectin-1 Mucosa Small bowel caspase galectin 1 galectin 1 polysaccharide animal cell animal experiment animal model apoptosis article binding site cell migration cell proliferation cell survival cell viability controlled study crypt cell glycosylation histopathology human human cell immunoblotting in vitro study in vivo study intestine cell intestine villus mitochondrial membrane potential mitochondrion mouse mutant nonhuman priority journal protein analysis protein expression protein function small intestine small intestine mucosa wild type animal cell death cell survival cytology epithelium cell genetics male metabolism mouse mutant Mus Animals Cell Death Cell Proliferation Cell Survival Epithelial Cells Galectin 1 Humans Intestine, Small Male Mice Mice, Knockout Polysaccharides |
| description |
Abstract: Intestinal epithelial cells serve as mechanical barriers and active components of the mucosal immune system. These cells migrate from the crypt to the tip of the villus, where different stimuli can differentially affect their survival. Here we investigated, using in vitro and in vivo strategies, the role of galectin-1 (Gal-1), an evolutionarily conserved glycan-binding protein, in modulating the survival of human and mouse enterocytes. Both Gal-1 and its specific glyco-receptors were broadly expressed in small bowel enterocytes. Exogenous Gal-1 reduced the viability of enterocytes through apoptotic mechanisms involving activation of both caspase and mitochondrial pathways. Consistent with these findings, apoptotic cells were mainly detected at the tip of the villi, following administration of Gal-1. Moreover, Gal-1-deficient (Lgals-1-) mice showed longer villi compared with their wild-type counterparts in vivo. In an experimental model of starvation, fasted wild-type mice displayed reduced villi and lower intestinal weight compared with Lgals-1- mutant mice, an effect reflected by changes in the frequency of enterocyte apoptosis. Of note, human small bowel enterocytes were also prone to this pro-apoptotic effect. Thus, Gal-1 is broadly expressed in mucosal tissue and influences the viability of human and mouse enterocytes, an effect which might influence the migration of these cells from the crypt, the integrity of the villus and the epithelial barrier function. © 2011 Macmillan Publishers Limited All rights reserved. |
| author |
Toscano, Marta Alicia Pozner, Roberto Gabriel |
| author_facet |
Toscano, Marta Alicia Pozner, Roberto Gabriel |
| author_sort |
Toscano, Marta Alicia |
| title |
The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine |
| title_short |
The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine |
| title_full |
The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine |
| title_fullStr |
The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine |
| title_full_unstemmed |
The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine |
| title_sort |
glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine |
| publishDate |
2011 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20414889_v2_n5_p_Muglia http://hdl.handle.net/20.500.12110/paper_20414889_v2_n5_p_Muglia |
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| _version_ |
1768546650976944128 |