Cytogenetic analysis on geographically distant parthenogenetic populations of Tityus trivittatus Kraepelin, 1898 (Scorpiones, Buthidae): Karyotype, constitutive heterochromatin and RDNA localization

Tityus trivittatus Kraepelin, 1898 is the most medically important scorpion species of Argentina, and parthenogenetic populations are present in the major cities of this country. We performed a detailed cytogenetic analysis of specimens of three synanthropic parthenogenetic populations, all distant...

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Autores principales: Adilardi, Renzo Sebastian, Mola, Liliana Maria
Publicado: 2014
Materias:
FISH
Holokinetic chromosomes
Karyotype
NOR
Parthenogenesis
Scorpion
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19930771_v8_n2_p81_Adilardi
http://hdl.handle.net/20.500.12110/paper_19930771_v8_n2_p81_Adilardi
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling paper:paper_19930771_v8_n2_p81_Adilardi2023-06-08T16:32:51Z Cytogenetic analysis on geographically distant parthenogenetic populations of Tityus trivittatus Kraepelin, 1898 (Scorpiones, Buthidae): Karyotype, constitutive heterochromatin and RDNA localization Adilardi, Renzo Sebastian Mola, Liliana Maria FISH Holokinetic chromosomes Karyotype NOR Parthenogenesis Scorpion Tityus trivittatus Kraepelin, 1898 is the most medically important scorpion species of Argentina, and parthenogenetic populations are present in the major cities of this country. We performed a detailed cytogenetic analysis of specimens of three synanthropic parthenogenetic populations, all distant about 900 km from each other, using Ag-NOR, C-banding, DAPI/CMA 3 staining and FISH with autologous 28S rDNA probes. The karyotype of females and embryos from the three populations showed 2n=6, with two large and four middle-sized holokinetic chromosomes. Constitutive heterochromatin was found in terminal and interstitial location and its pattern allowed the identification of three chromosome pairs. NORs were found on the terminal heterochromatic region of one pair of middle-sized chromosomes. The use of fluorochromes to characterize heterochromatin showed the absence of GC-rich heterochromatin and a low and variable number of AT-rich heterochromatic regions. We propose that a possible explanation for the lack of karyotypic variation between these geographically distant populations could be a recent colonization of urban areas by human means of synanthropic specimens from a single lineage of northeastern Argentina. Fil:Adilardi, R.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mola, L.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19930771_v8_n2_p81_Adilardi http://hdl.handle.net/20.500.12110/paper_19930771_v8_n2_p81_Adilardi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic FISH
Holokinetic chromosomes
Karyotype
NOR
Parthenogenesis
Scorpion
spellingShingle FISH
Holokinetic chromosomes
Karyotype
NOR
Parthenogenesis
Scorpion
Adilardi, Renzo Sebastian
Mola, Liliana Maria
Cytogenetic analysis on geographically distant parthenogenetic populations of Tityus trivittatus Kraepelin, 1898 (Scorpiones, Buthidae): Karyotype, constitutive heterochromatin and RDNA localization
topic_facet FISH
Holokinetic chromosomes
Karyotype
NOR
Parthenogenesis
Scorpion
description Tityus trivittatus Kraepelin, 1898 is the most medically important scorpion species of Argentina, and parthenogenetic populations are present in the major cities of this country. We performed a detailed cytogenetic analysis of specimens of three synanthropic parthenogenetic populations, all distant about 900 km from each other, using Ag-NOR, C-banding, DAPI/CMA 3 staining and FISH with autologous 28S rDNA probes. The karyotype of females and embryos from the three populations showed 2n=6, with two large and four middle-sized holokinetic chromosomes. Constitutive heterochromatin was found in terminal and interstitial location and its pattern allowed the identification of three chromosome pairs. NORs were found on the terminal heterochromatic region of one pair of middle-sized chromosomes. The use of fluorochromes to characterize heterochromatin showed the absence of GC-rich heterochromatin and a low and variable number of AT-rich heterochromatic regions. We propose that a possible explanation for the lack of karyotypic variation between these geographically distant populations could be a recent colonization of urban areas by human means of synanthropic specimens from a single lineage of northeastern Argentina.
author Adilardi, Renzo Sebastian
Mola, Liliana Maria
author_facet Adilardi, Renzo Sebastian
Mola, Liliana Maria
author_sort Adilardi, Renzo Sebastian
title Cytogenetic analysis on geographically distant parthenogenetic populations of Tityus trivittatus Kraepelin, 1898 (Scorpiones, Buthidae): Karyotype, constitutive heterochromatin and RDNA localization
title_short Cytogenetic analysis on geographically distant parthenogenetic populations of Tityus trivittatus Kraepelin, 1898 (Scorpiones, Buthidae): Karyotype, constitutive heterochromatin and RDNA localization
title_full Cytogenetic analysis on geographically distant parthenogenetic populations of Tityus trivittatus Kraepelin, 1898 (Scorpiones, Buthidae): Karyotype, constitutive heterochromatin and RDNA localization
title_fullStr Cytogenetic analysis on geographically distant parthenogenetic populations of Tityus trivittatus Kraepelin, 1898 (Scorpiones, Buthidae): Karyotype, constitutive heterochromatin and RDNA localization
title_full_unstemmed Cytogenetic analysis on geographically distant parthenogenetic populations of Tityus trivittatus Kraepelin, 1898 (Scorpiones, Buthidae): Karyotype, constitutive heterochromatin and RDNA localization
title_sort cytogenetic analysis on geographically distant parthenogenetic populations of tityus trivittatus kraepelin, 1898 (scorpiones, buthidae): karyotype, constitutive heterochromatin and rdna localization
publishDate 2014
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19930771_v8_n2_p81_Adilardi
http://hdl.handle.net/20.500.12110/paper_19930771_v8_n2_p81_Adilardi
work_keys_str_mv AT adilardirenzosebastian cytogeneticanalysisongeographicallydistantparthenogeneticpopulationsoftityustrivittatuskraepelin1898scorpionesbuthidaekaryotypeconstitutiveheterochromatinandrdnalocalization
AT molalilianamaria cytogeneticanalysisongeographicallydistantparthenogeneticpopulationsoftityustrivittatuskraepelin1898scorpionesbuthidaekaryotypeconstitutiveheterochromatinandrdnalocalization
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