RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes

Runx1 participation in epithelial mammary cells is still under review. Emerging data indicates that Runx1 could be relevant for breast tumor promotion. However, to date no studies have specifically evaluated the functional contribution of Runx1 to control gene expression in mammary epithelial tumor...

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Autores principales:	Echeverría, Pablo Christian, Rocha Viegas, Luciana, Schere Levy, Carolina P., Tocci, Johanna Melisa, Kordon, Edith Claudia, Rubinstein, Natalia
Publicado:	2016
Materias:	Foxp3 Gene expression regulation GJA1 Rspo3 Runx1 transcription factor FOXP3 transcription factor RUNX1 connexin 43 forkhead transcription factor FOXP3 protein, human GJA1 protein, human RSPO3 protein, human RUNX1 protein, human thrombospondin animal cell Article breast cancer breast cell breast epithelium cell cell migration controlled study down regulation FOXP3 gene gene activity gene expression gene interaction genetic transcription GJA1 gene in vitro study nonhuman pathogenesis promoter region RUNX1 gene tumor suppressor gene upregulation adenocarcinoma animal apoptosis Bagg albino mouse breast tumor cell motion cell proliferation chromatin immunoprecipitation confocal microscopy drug screening enzyme immunoassay female fluorescence microscopy fluorescent antibody technique gene expression regulation genetics human immunoprecipitation metabolism mouse pathology tumor cell culture Western blotting wound healing Adenocarcinoma Animals Apoptosis Blotting, Western Breast Neoplasms Cell Movement Cell Proliferation Chromatin Immunoprecipitation Connexin 43 Core Binding Factor Alpha 2 Subunit Female Fluorescent Antibody Technique Forkhead Transcription Factors Gene Expression Regulation, Neoplastic Humans Immunoenzyme Techniques Immunoprecipitation Mice Mice, Inbred BALB C Microscopy, Confocal Microscopy, Fluorescence Promoter Regions, Genetic Thrombospondins Tumor Cells, Cultured Wound Healing Xenograft Model Antitumor Assays
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v7_n6_p6552_Recouvreux http://hdl.handle.net/20.500.12110/paper_19492553_v7_n6_p6552_Recouvreux
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_19492553_v7_n6_p6552_Recouvreux
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spelling	paper:paper_19492553_v7_n6_p6552_Recouvreux2023-06-08T16:32:38Z RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes Echeverría, Pablo Christian Rocha Viegas, Luciana Schere Levy, Carolina P. Tocci, Johanna Melisa Kordon, Edith Claudia Rubinstein, Natalia Foxp3 Gene expression regulation GJA1 Rspo3 Runx1 transcription factor FOXP3 transcription factor RUNX1 connexin 43 forkhead transcription factor FOXP3 protein, human GJA1 protein, human RSPO3 protein, human RUNX1 protein, human thrombospondin transcription factor RUNX1 animal cell Article breast cancer breast cell breast epithelium cell cell migration controlled study down regulation FOXP3 gene gene activity gene expression gene interaction genetic transcription GJA1 gene in vitro study nonhuman pathogenesis promoter region RUNX1 gene tumor suppressor gene upregulation adenocarcinoma animal apoptosis Bagg albino mouse breast tumor cell motion cell proliferation chromatin immunoprecipitation confocal microscopy drug screening enzyme immunoassay female fluorescence microscopy fluorescent antibody technique gene expression regulation genetics human immunoprecipitation metabolism mouse pathology tumor cell culture Western blotting wound healing Adenocarcinoma Animals Apoptosis Blotting, Western Breast Neoplasms Cell Movement Cell Proliferation Chromatin Immunoprecipitation Connexin 43 Core Binding Factor Alpha 2 Subunit Female Fluorescent Antibody Technique Forkhead Transcription Factors Gene Expression Regulation, Neoplastic Humans Immunoenzyme Techniques Immunoprecipitation Mice Mice, Inbred BALB C Microscopy, Confocal Microscopy, Fluorescence Promoter Regions, Genetic Thrombospondins Tumor Cells, Cultured Wound Healing Xenograft Model Antitumor Assays Runx1 participation in epithelial mammary cells is still under review. Emerging data indicates that Runx1 could be relevant for breast tumor promotion. However, to date no studies have specifically evaluated the functional contribution of Runx1 to control gene expression in mammary epithelial tumor cells. It has been described that Runx1 activity is defined by protein context interaction. Interestingly, Foxp3 is a breast tumor suppressor gene. Here we show that endogenous Runx1 and Foxp3 physically interact in normal mammary cells and this interaction blocks Runx1 transcriptional activity. Furthermore we demonstrate that Runx1 is able to bind to R-spondin 3 (RSPO3) and Gap Junction protein Alpha 1 (GJA1) promoters. This binding upregulates Rspo3 oncogene expression and downregulates GJA1 tumor suppressor gene expression in a Foxp3-dependent manner. Moreover, reduced Runx1 transcriptional activity decreases tumor cell migration properties. Collectively, these data provide evidence of a new mechanism for breast tumor gene expression regulation, in which Runx1 and Foxp3 physically interact to control mammary epithelial cell gene expression fate. Our work suggests for the first time that Runx1 could be involved in breast tumor progression depending on Foxp3 availability. Fil:Echeverria, P.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rocha-Viegas, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Schere-Levy, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tocci, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kordon, E.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rubinstein, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v7_n6_p6552_Recouvreux http://hdl.handle.net/20.500.12110/paper_19492553_v7_n6_p6552_Recouvreux
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Foxp3 Gene expression regulation GJA1 Rspo3 Runx1 transcription factor FOXP3 transcription factor RUNX1 connexin 43 forkhead transcription factor FOXP3 protein, human GJA1 protein, human RSPO3 protein, human RUNX1 protein, human thrombospondin transcription factor RUNX1 animal cell Article breast cancer breast cell breast epithelium cell cell migration controlled study down regulation FOXP3 gene gene activity gene expression gene interaction genetic transcription GJA1 gene in vitro study nonhuman pathogenesis promoter region RUNX1 gene tumor suppressor gene upregulation adenocarcinoma animal apoptosis Bagg albino mouse breast tumor cell motion cell proliferation chromatin immunoprecipitation confocal microscopy drug screening enzyme immunoassay female fluorescence microscopy fluorescent antibody technique gene expression regulation genetics human immunoprecipitation metabolism mouse pathology tumor cell culture Western blotting wound healing Adenocarcinoma Animals Apoptosis Blotting, Western Breast Neoplasms Cell Movement Cell Proliferation Chromatin Immunoprecipitation Connexin 43 Core Binding Factor Alpha 2 Subunit Female Fluorescent Antibody Technique Forkhead Transcription Factors Gene Expression Regulation, Neoplastic Humans Immunoenzyme Techniques Immunoprecipitation Mice Mice, Inbred BALB C Microscopy, Confocal Microscopy, Fluorescence Promoter Regions, Genetic Thrombospondins Tumor Cells, Cultured Wound Healing Xenograft Model Antitumor Assays
spellingShingle	Foxp3 Gene expression regulation GJA1 Rspo3 Runx1 transcription factor FOXP3 transcription factor RUNX1 connexin 43 forkhead transcription factor FOXP3 protein, human GJA1 protein, human RSPO3 protein, human RUNX1 protein, human thrombospondin transcription factor RUNX1 animal cell Article breast cancer breast cell breast epithelium cell cell migration controlled study down regulation FOXP3 gene gene activity gene expression gene interaction genetic transcription GJA1 gene in vitro study nonhuman pathogenesis promoter region RUNX1 gene tumor suppressor gene upregulation adenocarcinoma animal apoptosis Bagg albino mouse breast tumor cell motion cell proliferation chromatin immunoprecipitation confocal microscopy drug screening enzyme immunoassay female fluorescence microscopy fluorescent antibody technique gene expression regulation genetics human immunoprecipitation metabolism mouse pathology tumor cell culture Western blotting wound healing Adenocarcinoma Animals Apoptosis Blotting, Western Breast Neoplasms Cell Movement Cell Proliferation Chromatin Immunoprecipitation Connexin 43 Core Binding Factor Alpha 2 Subunit Female Fluorescent Antibody Technique Forkhead Transcription Factors Gene Expression Regulation, Neoplastic Humans Immunoenzyme Techniques Immunoprecipitation Mice Mice, Inbred BALB C Microscopy, Confocal Microscopy, Fluorescence Promoter Regions, Genetic Thrombospondins Tumor Cells, Cultured Wound Healing Xenograft Model Antitumor Assays Echeverría, Pablo Christian Rocha Viegas, Luciana Schere Levy, Carolina P. Tocci, Johanna Melisa Kordon, Edith Claudia Rubinstein, Natalia RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes
topic_facet	Foxp3 Gene expression regulation GJA1 Rspo3 Runx1 transcription factor FOXP3 transcription factor RUNX1 connexin 43 forkhead transcription factor FOXP3 protein, human GJA1 protein, human RSPO3 protein, human RUNX1 protein, human thrombospondin transcription factor RUNX1 animal cell Article breast cancer breast cell breast epithelium cell cell migration controlled study down regulation FOXP3 gene gene activity gene expression gene interaction genetic transcription GJA1 gene in vitro study nonhuman pathogenesis promoter region RUNX1 gene tumor suppressor gene upregulation adenocarcinoma animal apoptosis Bagg albino mouse breast tumor cell motion cell proliferation chromatin immunoprecipitation confocal microscopy drug screening enzyme immunoassay female fluorescence microscopy fluorescent antibody technique gene expression regulation genetics human immunoprecipitation metabolism mouse pathology tumor cell culture Western blotting wound healing Adenocarcinoma Animals Apoptosis Blotting, Western Breast Neoplasms Cell Movement Cell Proliferation Chromatin Immunoprecipitation Connexin 43 Core Binding Factor Alpha 2 Subunit Female Fluorescent Antibody Technique Forkhead Transcription Factors Gene Expression Regulation, Neoplastic Humans Immunoenzyme Techniques Immunoprecipitation Mice Mice, Inbred BALB C Microscopy, Confocal Microscopy, Fluorescence Promoter Regions, Genetic Thrombospondins Tumor Cells, Cultured Wound Healing Xenograft Model Antitumor Assays
description	Runx1 participation in epithelial mammary cells is still under review. Emerging data indicates that Runx1 could be relevant for breast tumor promotion. However, to date no studies have specifically evaluated the functional contribution of Runx1 to control gene expression in mammary epithelial tumor cells. It has been described that Runx1 activity is defined by protein context interaction. Interestingly, Foxp3 is a breast tumor suppressor gene. Here we show that endogenous Runx1 and Foxp3 physically interact in normal mammary cells and this interaction blocks Runx1 transcriptional activity. Furthermore we demonstrate that Runx1 is able to bind to R-spondin 3 (RSPO3) and Gap Junction protein Alpha 1 (GJA1) promoters. This binding upregulates Rspo3 oncogene expression and downregulates GJA1 tumor suppressor gene expression in a Foxp3-dependent manner. Moreover, reduced Runx1 transcriptional activity decreases tumor cell migration properties. Collectively, these data provide evidence of a new mechanism for breast tumor gene expression regulation, in which Runx1 and Foxp3 physically interact to control mammary epithelial cell gene expression fate. Our work suggests for the first time that Runx1 could be involved in breast tumor progression depending on Foxp3 availability.
author	Echeverría, Pablo Christian Rocha Viegas, Luciana Schere Levy, Carolina P. Tocci, Johanna Melisa Kordon, Edith Claudia Rubinstein, Natalia
author_facet	Echeverría, Pablo Christian Rocha Viegas, Luciana Schere Levy, Carolina P. Tocci, Johanna Melisa Kordon, Edith Claudia Rubinstein, Natalia
author_sort	Echeverría, Pablo Christian
title	RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes
title_short	RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes
title_full	RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes
title_fullStr	RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes
title_full_unstemmed	RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes
title_sort	runx1 and foxp3 interplay regulates expression of breast cancer related genes
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v7_n6_p6552_Recouvreux http://hdl.handle.net/20.500.12110/paper_19492553_v7_n6_p6552_Recouvreux
work_keys_str_mv	AT echeverriapablochristian runx1andfoxp3interplayregulatesexpressionofbreastcancerrelatedgenes AT rochaviegasluciana runx1andfoxp3interplayregulatesexpressionofbreastcancerrelatedgenes AT scherelevycarolinap runx1andfoxp3interplayregulatesexpressionofbreastcancerrelatedgenes AT toccijohannamelisa runx1andfoxp3interplayregulatesexpressionofbreastcancerrelatedgenes AT kordonedithclaudia runx1andfoxp3interplayregulatesexpressionofbreastcancerrelatedgenes AT rubinsteinnatalia runx1andfoxp3interplayregulatesexpressionofbreastcancerrelatedgenes
_version_	1768541960422817792

RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes

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