Reprogramming human A375 amelanotic melanoma cells by catalase overexpression: Upregulation of antioxidant genes correlates with regression of melanoma malignancy and with malignant progression when downregulated
Reactive oxygen species (ROS) are implicated in tumor transformation. The antioxidant system (AOS) protects cells from ROS damage. However, it is also hijacked by cancers cells to proliferate within the tumor. Thus, identifying proteins altered by redox imbalance in cancer cells is an attractive pro...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v7_n27_p41154_Bracalente http://hdl.handle.net/20.500.12110/paper_19492553_v7_n27_p41154_Bracalente |
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paper:paper_19492553_v7_n27_p41154_Bracalente2023-06-08T16:32:37Z Reprogramming human A375 amelanotic melanoma cells by catalase overexpression: Upregulation of antioxidant genes correlates with regression of melanoma malignancy and with malignant progression when downregulated AOS network Melanogenesis Melanoma Metastasis Microarrays antioxidant catalase contactin 1 reactive oxygen metabolite tyrosinase related protein 1 ubiquitin catalase reactive oxygen metabolite transcriptome A-375 cell line animal cell antiproliferative activity Article cancer growth cancer prognosis cancer regression CNTN1 gene controlled study down regulation embryo EPHX2 gene gene gene expression profiling gene expression regulation gene overexpression GSR gene GSTM3 gene human human cell hydrogen peroxide scavenging assay melanogenesis melanoma metastatic melanoma MGST1 gene MGST3 gene mouse MSRA gene nonhuman nuclear reprogramming oxidative stress pathophysiology phenotype tumor cell TXNRD3 gene TYRP1 gene UCHL1 gene upregulation amelanotic melanoma cell proliferation disease exacerbation gene expression regulation genetics metabolism metastasis microarray analysis pathology skin tumor tumor cell line Antioxidants Catalase Cell Line, Tumor Cell Proliferation Disease Progression Down-Regulation Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Melanoma, Amelanotic Microarray Analysis Neoplasm Metastasis Oxidative Stress Reactive Oxygen Species Skin Neoplasms Transcriptome Up-Regulation Reactive oxygen species (ROS) are implicated in tumor transformation. The antioxidant system (AOS) protects cells from ROS damage. However, it is also hijacked by cancers cells to proliferate within the tumor. Thus, identifying proteins altered by redox imbalance in cancer cells is an attractive prognostic and therapeutic tool. Gene expression microarrays in A375 melanoma cells with different ROS levels after overexpressing catalase were performed. Dissimilar phenotypes by differential compensation to hydrogen peroxide scavenging were generated. The melanotic A375-A7 (A7) upregulated TYRP1, CNTN1 and UCHL1 promoting melanogenesis. The metastatic A375-G10 (G10) downregulated MTSS1 and TIAM1, proteins absent in metastasis. Moreover, differential coexpression of AOS genes (EPHX2, GSTM3, MGST1, MSRA, TXNRD3, MGST3 and GSR) was found in A7 and G10. Their increase in A7 improved its AOS ability and therefore, oxidative stress response, resembling less aggressive tumor cells. Meanwhile, their decrease in G10 revealed a disruption in the AOS and therefore, enhanced its metastatic capacity. These gene signatures, not only bring new insights into the physiopathology of melanoma, but also could be relevant in clinical prognostic to classify between non aggressive and metastatic melanomas. © 2018 Impact Journals. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v7_n27_p41154_Bracalente http://hdl.handle.net/20.500.12110/paper_19492553_v7_n27_p41154_Bracalente |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
AOS network Melanogenesis Melanoma Metastasis Microarrays antioxidant catalase contactin 1 reactive oxygen metabolite tyrosinase related protein 1 ubiquitin catalase reactive oxygen metabolite transcriptome A-375 cell line animal cell antiproliferative activity Article cancer growth cancer prognosis cancer regression CNTN1 gene controlled study down regulation embryo EPHX2 gene gene gene expression profiling gene expression regulation gene overexpression GSR gene GSTM3 gene human human cell hydrogen peroxide scavenging assay melanogenesis melanoma metastatic melanoma MGST1 gene MGST3 gene mouse MSRA gene nonhuman nuclear reprogramming oxidative stress pathophysiology phenotype tumor cell TXNRD3 gene TYRP1 gene UCHL1 gene upregulation amelanotic melanoma cell proliferation disease exacerbation gene expression regulation genetics metabolism metastasis microarray analysis pathology skin tumor tumor cell line Antioxidants Catalase Cell Line, Tumor Cell Proliferation Disease Progression Down-Regulation Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Melanoma, Amelanotic Microarray Analysis Neoplasm Metastasis Oxidative Stress Reactive Oxygen Species Skin Neoplasms Transcriptome Up-Regulation |
| spellingShingle |
AOS network Melanogenesis Melanoma Metastasis Microarrays antioxidant catalase contactin 1 reactive oxygen metabolite tyrosinase related protein 1 ubiquitin catalase reactive oxygen metabolite transcriptome A-375 cell line animal cell antiproliferative activity Article cancer growth cancer prognosis cancer regression CNTN1 gene controlled study down regulation embryo EPHX2 gene gene gene expression profiling gene expression regulation gene overexpression GSR gene GSTM3 gene human human cell hydrogen peroxide scavenging assay melanogenesis melanoma metastatic melanoma MGST1 gene MGST3 gene mouse MSRA gene nonhuman nuclear reprogramming oxidative stress pathophysiology phenotype tumor cell TXNRD3 gene TYRP1 gene UCHL1 gene upregulation amelanotic melanoma cell proliferation disease exacerbation gene expression regulation genetics metabolism metastasis microarray analysis pathology skin tumor tumor cell line Antioxidants Catalase Cell Line, Tumor Cell Proliferation Disease Progression Down-Regulation Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Melanoma, Amelanotic Microarray Analysis Neoplasm Metastasis Oxidative Stress Reactive Oxygen Species Skin Neoplasms Transcriptome Up-Regulation Reprogramming human A375 amelanotic melanoma cells by catalase overexpression: Upregulation of antioxidant genes correlates with regression of melanoma malignancy and with malignant progression when downregulated |
| topic_facet |
AOS network Melanogenesis Melanoma Metastasis Microarrays antioxidant catalase contactin 1 reactive oxygen metabolite tyrosinase related protein 1 ubiquitin catalase reactive oxygen metabolite transcriptome A-375 cell line animal cell antiproliferative activity Article cancer growth cancer prognosis cancer regression CNTN1 gene controlled study down regulation embryo EPHX2 gene gene gene expression profiling gene expression regulation gene overexpression GSR gene GSTM3 gene human human cell hydrogen peroxide scavenging assay melanogenesis melanoma metastatic melanoma MGST1 gene MGST3 gene mouse MSRA gene nonhuman nuclear reprogramming oxidative stress pathophysiology phenotype tumor cell TXNRD3 gene TYRP1 gene UCHL1 gene upregulation amelanotic melanoma cell proliferation disease exacerbation gene expression regulation genetics metabolism metastasis microarray analysis pathology skin tumor tumor cell line Antioxidants Catalase Cell Line, Tumor Cell Proliferation Disease Progression Down-Regulation Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Melanoma, Amelanotic Microarray Analysis Neoplasm Metastasis Oxidative Stress Reactive Oxygen Species Skin Neoplasms Transcriptome Up-Regulation |
| description |
Reactive oxygen species (ROS) are implicated in tumor transformation. The antioxidant system (AOS) protects cells from ROS damage. However, it is also hijacked by cancers cells to proliferate within the tumor. Thus, identifying proteins altered by redox imbalance in cancer cells is an attractive prognostic and therapeutic tool. Gene expression microarrays in A375 melanoma cells with different ROS levels after overexpressing catalase were performed. Dissimilar phenotypes by differential compensation to hydrogen peroxide scavenging were generated. The melanotic A375-A7 (A7) upregulated TYRP1, CNTN1 and UCHL1 promoting melanogenesis. The metastatic A375-G10 (G10) downregulated MTSS1 and TIAM1, proteins absent in metastasis. Moreover, differential coexpression of AOS genes (EPHX2, GSTM3, MGST1, MSRA, TXNRD3, MGST3 and GSR) was found in A7 and G10. Their increase in A7 improved its AOS ability and therefore, oxidative stress response, resembling less aggressive tumor cells. Meanwhile, their decrease in G10 revealed a disruption in the AOS and therefore, enhanced its metastatic capacity. These gene signatures, not only bring new insights into the physiopathology of melanoma, but also could be relevant in clinical prognostic to classify between non aggressive and metastatic melanomas. © 2018 Impact Journals. |
| title |
Reprogramming human A375 amelanotic melanoma cells by catalase overexpression: Upregulation of antioxidant genes correlates with regression of melanoma malignancy and with malignant progression when downregulated |
| title_short |
Reprogramming human A375 amelanotic melanoma cells by catalase overexpression: Upregulation of antioxidant genes correlates with regression of melanoma malignancy and with malignant progression when downregulated |
| title_full |
Reprogramming human A375 amelanotic melanoma cells by catalase overexpression: Upregulation of antioxidant genes correlates with regression of melanoma malignancy and with malignant progression when downregulated |
| title_fullStr |
Reprogramming human A375 amelanotic melanoma cells by catalase overexpression: Upregulation of antioxidant genes correlates with regression of melanoma malignancy and with malignant progression when downregulated |
| title_full_unstemmed |
Reprogramming human A375 amelanotic melanoma cells by catalase overexpression: Upregulation of antioxidant genes correlates with regression of melanoma malignancy and with malignant progression when downregulated |
| title_sort |
reprogramming human a375 amelanotic melanoma cells by catalase overexpression: upregulation of antioxidant genes correlates with regression of melanoma malignancy and with malignant progression when downregulated |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v7_n27_p41154_Bracalente http://hdl.handle.net/20.500.12110/paper_19492553_v7_n27_p41154_Bracalente |
| _version_ |
1768542908343910400 |