Two-photon optical interrogation of individual dendritic spines with caged dopamine

We introduce a novel caged dopamine compound (RuBi-Dopa) based on ruthenium photochemistry. RuBi-Dopa has a high uncaging efficiency and can be released with visible (blue-green) and IR light in a two-photon regime. We combine two-photon photorelease of RuBi-Dopa with two-photon calcium imaging for...

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Detalles Bibliográficos
Autor principal: Etchenique, Roberto
Publicado: 2013
Materias:
caged compound
Dopamine
ruthenium
two-photon
bipyridine
calcium
dopamine
dopamine receptor
animal cell
article
brain slice
dendritic spine
fluorescence imaging
infrared photography
mouse
nonhuman
photochemistry
photon
priority journal
protein expression
quantum yield
Animals
Calcium Isotopes
Dendritic Spines
Mice
Photolysis
Photons
Prefrontal Cortex
Receptors, Dopamine
Ruthenium
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19487193_v4_n8_p1163_Araya
http://hdl.handle.net/20.500.12110/paper_19487193_v4_n8_p1163_Araya
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:We introduce a novel caged dopamine compound (RuBi-Dopa) based on ruthenium photochemistry. RuBi-Dopa has a high uncaging efficiency and can be released with visible (blue-green) and IR light in a two-photon regime. We combine two-photon photorelease of RuBi-Dopa with two-photon calcium imaging for an optical imaging and manipulation of dendritic spines in living brain slices, demonstrating that spines can express functional dopamine receptors. This novel compound allows mapping of functional dopamine receptors in living brain tissue with exquisite spatial resolution. © 2013 American Chemical Society.

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