Two-photon optical interrogation of individual dendritic spines with caged dopamine
We introduce a novel caged dopamine compound (RuBi-Dopa) based on ruthenium photochemistry. RuBi-Dopa has a high uncaging efficiency and can be released with visible (blue-green) and IR light in a two-photon regime. We combine two-photon photorelease of RuBi-Dopa with two-photon calcium imaging for...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19487193_v4_n8_p1163_Araya http://hdl.handle.net/20.500.12110/paper_19487193_v4_n8_p1163_Araya |
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paper:paper_19487193_v4_n8_p1163_Araya2023-06-08T16:32:35Z Two-photon optical interrogation of individual dendritic spines with caged dopamine Etchenique, Roberto caged compound Dopamine ruthenium two-photon bipyridine calcium dopamine dopamine receptor ruthenium animal cell article brain slice dendritic spine fluorescence imaging infrared photography mouse nonhuman photochemistry photon priority journal protein expression quantum yield Animals Calcium Isotopes Dendritic Spines Dopamine Mice Photolysis Photons Prefrontal Cortex Receptors, Dopamine Ruthenium We introduce a novel caged dopamine compound (RuBi-Dopa) based on ruthenium photochemistry. RuBi-Dopa has a high uncaging efficiency and can be released with visible (blue-green) and IR light in a two-photon regime. We combine two-photon photorelease of RuBi-Dopa with two-photon calcium imaging for an optical imaging and manipulation of dendritic spines in living brain slices, demonstrating that spines can express functional dopamine receptors. This novel compound allows mapping of functional dopamine receptors in living brain tissue with exquisite spatial resolution. © 2013 American Chemical Society. Fil:Etchenique, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19487193_v4_n8_p1163_Araya http://hdl.handle.net/20.500.12110/paper_19487193_v4_n8_p1163_Araya |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
caged compound Dopamine ruthenium two-photon bipyridine calcium dopamine dopamine receptor ruthenium animal cell article brain slice dendritic spine fluorescence imaging infrared photography mouse nonhuman photochemistry photon priority journal protein expression quantum yield Animals Calcium Isotopes Dendritic Spines Dopamine Mice Photolysis Photons Prefrontal Cortex Receptors, Dopamine Ruthenium |
| spellingShingle |
caged compound Dopamine ruthenium two-photon bipyridine calcium dopamine dopamine receptor ruthenium animal cell article brain slice dendritic spine fluorescence imaging infrared photography mouse nonhuman photochemistry photon priority journal protein expression quantum yield Animals Calcium Isotopes Dendritic Spines Dopamine Mice Photolysis Photons Prefrontal Cortex Receptors, Dopamine Ruthenium Etchenique, Roberto Two-photon optical interrogation of individual dendritic spines with caged dopamine |
| topic_facet |
caged compound Dopamine ruthenium two-photon bipyridine calcium dopamine dopamine receptor ruthenium animal cell article brain slice dendritic spine fluorescence imaging infrared photography mouse nonhuman photochemistry photon priority journal protein expression quantum yield Animals Calcium Isotopes Dendritic Spines Dopamine Mice Photolysis Photons Prefrontal Cortex Receptors, Dopamine Ruthenium |
| description |
We introduce a novel caged dopamine compound (RuBi-Dopa) based on ruthenium photochemistry. RuBi-Dopa has a high uncaging efficiency and can be released with visible (blue-green) and IR light in a two-photon regime. We combine two-photon photorelease of RuBi-Dopa with two-photon calcium imaging for an optical imaging and manipulation of dendritic spines in living brain slices, demonstrating that spines can express functional dopamine receptors. This novel compound allows mapping of functional dopamine receptors in living brain tissue with exquisite spatial resolution. © 2013 American Chemical Society. |
| author |
Etchenique, Roberto |
| author_facet |
Etchenique, Roberto |
| author_sort |
Etchenique, Roberto |
| title |
Two-photon optical interrogation of individual dendritic spines with caged dopamine |
| title_short |
Two-photon optical interrogation of individual dendritic spines with caged dopamine |
| title_full |
Two-photon optical interrogation of individual dendritic spines with caged dopamine |
| title_fullStr |
Two-photon optical interrogation of individual dendritic spines with caged dopamine |
| title_full_unstemmed |
Two-photon optical interrogation of individual dendritic spines with caged dopamine |
| title_sort |
two-photon optical interrogation of individual dendritic spines with caged dopamine |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19487193_v4_n8_p1163_Araya http://hdl.handle.net/20.500.12110/paper_19487193_v4_n8_p1163_Araya |
| work_keys_str_mv |
AT etcheniqueroberto twophotonopticalinterrogationofindividualdendriticspineswithcageddopamine |
| _version_ |
1768545116322004992 |