Role of histamine H4 receptor in breast cancer cell proliferation
In order to better understand the role of histamine H4 (H4R) receptor in breast cancer, we studied the receptor expression pattern, associated signal transduction pathway and biological responses, in breast cancer cell lines with different malignant characteristics. A different pattern of protein ex...
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2011
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19450494_v3E_n3_p1042_Medina http://hdl.handle.net/20.500.12110/paper_19450494_v3E_n3_p1042_Medina |
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paper:paper_19450494_v3E_n3_p1042_Medina2023-06-08T16:32:28Z Role of histamine H4 receptor in breast cancer cell proliferation Apoptosis Cell Senescence Histamine H4 Receptor Human Breast Cancer Proliferation 1 (5 chloro 2 indolylcarbonyl) 4 methylpiperazine 1 [2 (amidinothio)ethyl]guanidine clobenpropit cyclic AMP histamine histamine H3 receptor antagonist histamine H4 receptor lipocortin 5 unclassified drug G protein coupled receptor histamine receptor HRH4 protein, human primer DNA apoptosis article breast cancer cancer cell culture cell aging cell proliferation controlled study human human cell nick end labeling protein expression signal transduction breast tumor female nucleotide sequence pathology physiology reverse transcription polymerase chain reaction tumor cell line Western blotting Apoptosis Base Sequence Blotting, Western Breast Neoplasms Cell Line, Tumor Cell Proliferation DNA Primers Female Humans In Situ Nick-End Labeling Receptors, G-Protein-Coupled Receptors, Histamine Reverse Transcriptase Polymerase Chain Reaction In order to better understand the role of histamine H4 (H4R) receptor in breast cancer, we studied the receptor expression pattern, associated signal transduction pathway and biological responses, in breast cancer cell lines with different malignant characteristics. A different pattern of protein expression was observed in MDA-MB-231 compared to MCF-7 cells determined by western blot, exhibiting the presence of a diverse range of molecular weight species of the H4R. H4R agonist reduced cyclic adenosine monophosphate (cAMP) formation induced by forskolin only in MCF-7 cells. In MDA-MB-231 cells, H4R agonists significantly decreased cell roliferation, augmented the Annexin-V and TdT-mediated UTP-biotin Nick End labelling (TUNEL) positive cells and produced a 2.5-fold increase in cell senescence. In MCF-7 cells, H4R agonists inhibited proliferation by 50%, increasing the exponential doubling time. This effect was associated to an augment in Annexin-V and TUNEL positive cells, and a 2-fold increase in cell senescence. We conclude that H4R is functionally expressed in human breast cancer cell lines, exhibiting a key role in histaminemediated biological processes such as cell proliferation, senescence and apoptosis. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19450494_v3E_n3_p1042_Medina http://hdl.handle.net/20.500.12110/paper_19450494_v3E_n3_p1042_Medina |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Apoptosis Cell Senescence Histamine H4 Receptor Human Breast Cancer Proliferation 1 (5 chloro 2 indolylcarbonyl) 4 methylpiperazine 1 [2 (amidinothio)ethyl]guanidine clobenpropit cyclic AMP histamine histamine H3 receptor antagonist histamine H4 receptor lipocortin 5 unclassified drug G protein coupled receptor histamine receptor HRH4 protein, human primer DNA apoptosis article breast cancer cancer cell culture cell aging cell proliferation controlled study human human cell nick end labeling protein expression signal transduction breast tumor female nucleotide sequence pathology physiology reverse transcription polymerase chain reaction tumor cell line Western blotting Apoptosis Base Sequence Blotting, Western Breast Neoplasms Cell Line, Tumor Cell Proliferation DNA Primers Female Humans In Situ Nick-End Labeling Receptors, G-Protein-Coupled Receptors, Histamine Reverse Transcriptase Polymerase Chain Reaction |
| spellingShingle |
Apoptosis Cell Senescence Histamine H4 Receptor Human Breast Cancer Proliferation 1 (5 chloro 2 indolylcarbonyl) 4 methylpiperazine 1 [2 (amidinothio)ethyl]guanidine clobenpropit cyclic AMP histamine histamine H3 receptor antagonist histamine H4 receptor lipocortin 5 unclassified drug G protein coupled receptor histamine receptor HRH4 protein, human primer DNA apoptosis article breast cancer cancer cell culture cell aging cell proliferation controlled study human human cell nick end labeling protein expression signal transduction breast tumor female nucleotide sequence pathology physiology reverse transcription polymerase chain reaction tumor cell line Western blotting Apoptosis Base Sequence Blotting, Western Breast Neoplasms Cell Line, Tumor Cell Proliferation DNA Primers Female Humans In Situ Nick-End Labeling Receptors, G-Protein-Coupled Receptors, Histamine Reverse Transcriptase Polymerase Chain Reaction Role of histamine H4 receptor in breast cancer cell proliferation |
| topic_facet |
Apoptosis Cell Senescence Histamine H4 Receptor Human Breast Cancer Proliferation 1 (5 chloro 2 indolylcarbonyl) 4 methylpiperazine 1 [2 (amidinothio)ethyl]guanidine clobenpropit cyclic AMP histamine histamine H3 receptor antagonist histamine H4 receptor lipocortin 5 unclassified drug G protein coupled receptor histamine receptor HRH4 protein, human primer DNA apoptosis article breast cancer cancer cell culture cell aging cell proliferation controlled study human human cell nick end labeling protein expression signal transduction breast tumor female nucleotide sequence pathology physiology reverse transcription polymerase chain reaction tumor cell line Western blotting Apoptosis Base Sequence Blotting, Western Breast Neoplasms Cell Line, Tumor Cell Proliferation DNA Primers Female Humans In Situ Nick-End Labeling Receptors, G-Protein-Coupled Receptors, Histamine Reverse Transcriptase Polymerase Chain Reaction |
| description |
In order to better understand the role of histamine H4 (H4R) receptor in breast cancer, we studied the receptor expression pattern, associated signal transduction pathway and biological responses, in breast cancer cell lines with different malignant characteristics. A different pattern of protein expression was observed in MDA-MB-231 compared to MCF-7 cells determined by western blot, exhibiting the presence of a diverse range of molecular weight species of the H4R. H4R agonist reduced cyclic adenosine monophosphate (cAMP) formation induced by forskolin only in MCF-7 cells. In MDA-MB-231 cells, H4R agonists significantly decreased cell roliferation, augmented the Annexin-V and TdT-mediated UTP-biotin Nick End labelling (TUNEL) positive cells and produced a 2.5-fold increase in cell senescence. In MCF-7 cells, H4R agonists inhibited proliferation by 50%, increasing the exponential doubling time. This effect was associated to an augment in Annexin-V and TUNEL positive cells, and a 2-fold increase in cell senescence. We conclude that H4R is functionally expressed in human breast cancer cell lines, exhibiting a key role in histaminemediated biological processes such as cell proliferation, senescence and apoptosis. |
| title |
Role of histamine H4 receptor in breast cancer cell proliferation |
| title_short |
Role of histamine H4 receptor in breast cancer cell proliferation |
| title_full |
Role of histamine H4 receptor in breast cancer cell proliferation |
| title_fullStr |
Role of histamine H4 receptor in breast cancer cell proliferation |
| title_full_unstemmed |
Role of histamine H4 receptor in breast cancer cell proliferation |
| title_sort |
role of histamine h4 receptor in breast cancer cell proliferation |
| publishDate |
2011 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19450494_v3E_n3_p1042_Medina http://hdl.handle.net/20.500.12110/paper_19450494_v3E_n3_p1042_Medina |
| _version_ |
1768545259132813312 |