Survey of argentinean human plasma for ochratoxin a
The presence of ochratoxin A (OTA) in human blood has been reported for many countries, especially in Europe. However, so far no report exists concerning such a presence in Argentina. The aim of this study was to assess OTA concentration in human plasma in two different areas of Buenos Aires provinc...
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| Autores principales: | , |
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2008
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19440049_v25_n5_p635_Pacin http://hdl.handle.net/20.500.12110/paper_19440049_v25_n5_p635_Pacin |
| Aporte de: |
| Sumario: | The presence of ochratoxin A (OTA) in human blood has been reported for many countries, especially in Europe. However, so far no report exists concerning such a presence in Argentina. The aim of this study was to assess OTA concentration in human plasma in two different areas of Buenos Aires province. OTA was determined by high-performance liquid chromatography (HPLC) in 199 plasma samples from blood donors in Mar del Plata and 236 from General Rodríguez. Solid-phase extraction with Bakerbond® C-18 cartridge and a final purification with Ochraprep® immunoaffinity columns was employed. The limit of quantification of ochratoxin A was 0.019ngml−1 and the confirmation of OTA was by formation of ochratoxin A methyl ester. The results showed that 63.8% of human plasma samples from Mar del Plata and 62.3% from General Rodríguez were positive for OTA, with Winsorized means of 0.15 and 0.43ngml−1, respectively. It is important to continue the research to detect the foods responsible of the presence of OTA in plasma. © 2008 Taylor & Francis Group, LLC. |
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