Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity

The aim of this study was to identify and characterize pituitary cells of Steindachneridion parahybae females in captivity, highlighting the possible relationship with reproductive disorders at this level, since this species shows oocyte final maturation, ovulation and spawning dysfunction in captiv...

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Autores principales:	Pandolfi, Matías, Lo Nostro, Fabiana Laura
Publicado:	2015
Materias:	Follicle stimulating hormone Growth hormone Luteinizing hormone Prolactin hormone Reproductive dysfunction Somatolactin hormone follitropin beta subunit gonadotropin growth hormone luteinizing hormone beta subunit prolactin somatolactin unclassified drug fish protein adenohypophysis animal tissue Article cell structure cellular distribution controlled study feedback system female genital system disease hypophysis hypophysis cell immunohistochemistry nonhuman oocyte development oocyte maturation optical density ovary cycle ovary development ovulation priority journal reproductive success Siluriformes spawning teleost Western blotting animal catfish classification cytology embryology endangered species metabolism nonmammalian embryo oocyte reproduction vitellogenesis Pimelodidae Teleostei Animals Blotting, Western Catfishes Embryo, Nonmammalian Endangered Species Female Fish Proteins Gonadotropins Growth Hormone Immunohistochemistry Oocytes Pituitary Gland Reproduction Vitellogenesis
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19328486_v298_n9_p1644_Honji http://hdl.handle.net/20.500.12110/paper_19328486_v298_n9_p1644_Honji
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_19328486_v298_n9_p1644_Honji
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spelling	paper:paper_19328486_v298_n9_p1644_Honji2023-06-08T16:31:45Z Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity Pandolfi, Matías Lo Nostro, Fabiana Laura Follicle stimulating hormone Growth hormone Luteinizing hormone Prolactin hormone Reproductive dysfunction Somatolactin hormone follitropin beta subunit gonadotropin growth hormone luteinizing hormone beta subunit prolactin somatolactin unclassified drug fish protein gonadotropin growth hormone adenohypophysis animal tissue Article cell structure cellular distribution controlled study feedback system female genital system disease hypophysis hypophysis cell immunohistochemistry nonhuman oocyte development oocyte maturation optical density ovary cycle ovary development ovulation priority journal reproductive success Siluriformes spawning teleost Western blotting animal catfish classification cytology embryology endangered species metabolism nonmammalian embryo oocyte reproduction vitellogenesis Pimelodidae Siluriformes Teleostei Animals Blotting, Western Catfishes Embryo, Nonmammalian Endangered Species Female Fish Proteins Gonadotropins Growth Hormone Immunohistochemistry Oocytes Pituitary Gland Reproduction Vitellogenesis The aim of this study was to identify and characterize pituitary cells of Steindachneridion parahybae females in captivity, highlighting the possible relationship with reproductive disorders at this level, since this species shows oocyte final maturation, ovulation and spawning dysfunction in captivity. The localization and distribution of growth hormone (GH), prolactin (PRL), somatolactin (SL), β-luteinizing hormone (β-LH), and β-follicle stimulating hormone (β-FSH) immunoreactive (-ir) cells in the adenohypophysis was studied by immunohistochemical and Western blot methods. In addition, cellular morphometric analyses and semi-quantification of ir-cells optical density (OD) during the annual reproductive cycle and after artificial induced spawning (AIS) were performed. Results showed that the distribution and general localization of pituitary cell types were similar to that of other teleost species. However, the morphometrical study of adenohypophysial cells showed differences along the reproductive cycle and following AIS. In general, females at the vitellogenic stage presented greater OD values for GH, PRL and SL than at other maturation stages (previtellogenic and regression stages), probably indicating an increased cellular activity during this stage. Conversely, β-LH OD did not vary during the annual reproductive cycle. After AIS, β-LH, SL and GH ir-cells showed an increase in OD values suggesting a possible involvement on oocyte final maturation, ovulation and spawning or a feedback control on the brain-pituitary-gonads axis. Reproductive dysfunction in S. parahybae females in captivity may be due to alteration of the synthesis pathways of β-LH. In addition, GH family of hormones could modulate associated mechanisms that influence the reproductive status in this species. © 2015 Wiley Periodicals, Inc. Fil:Pandolfi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lo Nostro, F.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19328486_v298_n9_p1644_Honji http://hdl.handle.net/20.500.12110/paper_19328486_v298_n9_p1644_Honji
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Follicle stimulating hormone Growth hormone Luteinizing hormone Prolactin hormone Reproductive dysfunction Somatolactin hormone follitropin beta subunit gonadotropin growth hormone luteinizing hormone beta subunit prolactin somatolactin unclassified drug fish protein gonadotropin growth hormone adenohypophysis animal tissue Article cell structure cellular distribution controlled study feedback system female genital system disease hypophysis hypophysis cell immunohistochemistry nonhuman oocyte development oocyte maturation optical density ovary cycle ovary development ovulation priority journal reproductive success Siluriformes spawning teleost Western blotting animal catfish classification cytology embryology endangered species metabolism nonmammalian embryo oocyte reproduction vitellogenesis Pimelodidae Siluriformes Teleostei Animals Blotting, Western Catfishes Embryo, Nonmammalian Endangered Species Female Fish Proteins Gonadotropins Growth Hormone Immunohistochemistry Oocytes Pituitary Gland Reproduction Vitellogenesis
spellingShingle	Follicle stimulating hormone Growth hormone Luteinizing hormone Prolactin hormone Reproductive dysfunction Somatolactin hormone follitropin beta subunit gonadotropin growth hormone luteinizing hormone beta subunit prolactin somatolactin unclassified drug fish protein gonadotropin growth hormone adenohypophysis animal tissue Article cell structure cellular distribution controlled study feedback system female genital system disease hypophysis hypophysis cell immunohistochemistry nonhuman oocyte development oocyte maturation optical density ovary cycle ovary development ovulation priority journal reproductive success Siluriformes spawning teleost Western blotting animal catfish classification cytology embryology endangered species metabolism nonmammalian embryo oocyte reproduction vitellogenesis Pimelodidae Siluriformes Teleostei Animals Blotting, Western Catfishes Embryo, Nonmammalian Endangered Species Female Fish Proteins Gonadotropins Growth Hormone Immunohistochemistry Oocytes Pituitary Gland Reproduction Vitellogenesis Pandolfi, Matías Lo Nostro, Fabiana Laura Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity
topic_facet	Follicle stimulating hormone Growth hormone Luteinizing hormone Prolactin hormone Reproductive dysfunction Somatolactin hormone follitropin beta subunit gonadotropin growth hormone luteinizing hormone beta subunit prolactin somatolactin unclassified drug fish protein gonadotropin growth hormone adenohypophysis animal tissue Article cell structure cellular distribution controlled study feedback system female genital system disease hypophysis hypophysis cell immunohistochemistry nonhuman oocyte development oocyte maturation optical density ovary cycle ovary development ovulation priority journal reproductive success Siluriformes spawning teleost Western blotting animal catfish classification cytology embryology endangered species metabolism nonmammalian embryo oocyte reproduction vitellogenesis Pimelodidae Siluriformes Teleostei Animals Blotting, Western Catfishes Embryo, Nonmammalian Endangered Species Female Fish Proteins Gonadotropins Growth Hormone Immunohistochemistry Oocytes Pituitary Gland Reproduction Vitellogenesis
description	The aim of this study was to identify and characterize pituitary cells of Steindachneridion parahybae females in captivity, highlighting the possible relationship with reproductive disorders at this level, since this species shows oocyte final maturation, ovulation and spawning dysfunction in captivity. The localization and distribution of growth hormone (GH), prolactin (PRL), somatolactin (SL), β-luteinizing hormone (β-LH), and β-follicle stimulating hormone (β-FSH) immunoreactive (-ir) cells in the adenohypophysis was studied by immunohistochemical and Western blot methods. In addition, cellular morphometric analyses and semi-quantification of ir-cells optical density (OD) during the annual reproductive cycle and after artificial induced spawning (AIS) were performed. Results showed that the distribution and general localization of pituitary cell types were similar to that of other teleost species. However, the morphometrical study of adenohypophysial cells showed differences along the reproductive cycle and following AIS. In general, females at the vitellogenic stage presented greater OD values for GH, PRL and SL than at other maturation stages (previtellogenic and regression stages), probably indicating an increased cellular activity during this stage. Conversely, β-LH OD did not vary during the annual reproductive cycle. After AIS, β-LH, SL and GH ir-cells showed an increase in OD values suggesting a possible involvement on oocyte final maturation, ovulation and spawning or a feedback control on the brain-pituitary-gonads axis. Reproductive dysfunction in S. parahybae females in captivity may be due to alteration of the synthesis pathways of β-LH. In addition, GH family of hormones could modulate associated mechanisms that influence the reproductive status in this species. © 2015 Wiley Periodicals, Inc.
author	Pandolfi, Matías Lo Nostro, Fabiana Laura
author_facet	Pandolfi, Matías Lo Nostro, Fabiana Laura
author_sort	Pandolfi, Matías
title	Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity
title_short	Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity
title_full	Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity
title_fullStr	Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity
title_full_unstemmed	Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity
title_sort	gonadotropins and growth hormone family characterization in an endangered siluriform species, steindachneridion parahybae (pimelodidae): relationship with annual reproductive cycle and induced spawning in captivity
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19328486_v298_n9_p1644_Honji http://hdl.handle.net/20.500.12110/paper_19328486_v298_n9_p1644_Honji
work_keys_str_mv	AT pandolfimatias gonadotropinsandgrowthhormonefamilycharacterizationinanendangeredsiluriformspeciessteindachneridionparahybaepimelodidaerelationshipwithannualreproductivecycleandinducedspawningincaptivity AT lonostrofabianalaura gonadotropinsandgrowthhormonefamilycharacterizationinanendangeredsiluriformspeciessteindachneridionparahybaepimelodidaerelationshipwithannualreproductivecycleandinducedspawningincaptivity
_version_	1768546323758317568

Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity

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