Neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of Xenopus laevis
The olfactory epithelium (OE) has the remarkable capability to constantly replace olfactory receptor neurons (ORNs) due to the presence of neural stem cells (NSCs). For this reason, the OE provides an excellent model to study neurogenesis and neuronal differentiation. In the present work, we induced...
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2017
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paper:paper_19328451_v77_n11_p1308_Cervino2023-06-08T16:31:44Z Neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of Xenopus laevis neural stem cells neurodifferentiation neurogenesis olfaction olfactory receptor neurons neuromodulin neurotrophic factor neurotrophin brain derived neurotrophic factor caspase 3 cytokeratin 2 nerve cell adhesion molecule neuromodulin olfactory marker protein animal experiment animal tissue apoptosis Article axotomy basal cell cell proliferation controlled study embryo nerve cell degeneration nerve cell differentiation nerve cell necrosis nerve regeneration nervous system development nonhuman olfactory bulb olfactory epithelium olfactory nerve olfactory receptor neuron priority journal smelling Xenopus laevis animal cell differentiation convalescence gene expression regulation metabolism nerve degeneration odor olfactory mucosa olfactory nerve injury pathology physiology regeneration time factor Xenopus laevis Animals Axotomy Brain-Derived Neurotrophic Factor Caspase 3 Cell Differentiation Cell Proliferation GAP-43 Protein Gene Expression Regulation Keratin-2 Nerve Degeneration Neural Cell Adhesion Molecules Olfactory Marker Protein Olfactory Mucosa Olfactory Nerve Injuries Recovery of Function Regeneration Smell Time Factors Xenopus laevis The olfactory epithelium (OE) has the remarkable capability to constantly replace olfactory receptor neurons (ORNs) due to the presence of neural stem cells (NSCs). For this reason, the OE provides an excellent model to study neurogenesis and neuronal differentiation. In the present work, we induced neuronal degeneration in the OE of Xenopus laevis larvae by bilateral axotomy of the olfactory nerves. We found that axotomy induces specific- neuronal death through apoptosis between 24 and 48h post-injury. In concordance, there was a progressive decrease of the mature-ORN marker OMP until it was completely absent 72h post-injury. On the other hand, neurogenesis was evident 48h post-injury by an increase in the number of proliferating basal cells as well as NCAM-180– GAP-43+ immature neurons. Mature ORNs were replenished 21 days post-injury and the olfactory function was partially recovered, indicating that new ORNs were integrated into the olfactory bulb glomeruli. Throughout the regenerative process no changes in the expression pattern of the neurotrophin Brain Derivate Neurotrophic Factor were observed. Taken together, this work provides a sequential analysis of the neurodegenerative and subsequent regenerative processes that take place in the OE following axotomy. © 2017 Wiley Periodicals, Inc. Develop Neurobiol 77: 1308–1320, 2017. © 2017 Wiley Periodicals, Inc. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19328451_v77_n11_p1308_Cervino http://hdl.handle.net/20.500.12110/paper_19328451_v77_n11_p1308_Cervino |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
neural stem cells neurodifferentiation neurogenesis olfaction olfactory receptor neurons neuromodulin neurotrophic factor neurotrophin brain derived neurotrophic factor caspase 3 cytokeratin 2 nerve cell adhesion molecule neuromodulin olfactory marker protein animal experiment animal tissue apoptosis Article axotomy basal cell cell proliferation controlled study embryo nerve cell degeneration nerve cell differentiation nerve cell necrosis nerve regeneration nervous system development nonhuman olfactory bulb olfactory epithelium olfactory nerve olfactory receptor neuron priority journal smelling Xenopus laevis animal cell differentiation convalescence gene expression regulation metabolism nerve degeneration odor olfactory mucosa olfactory nerve injury pathology physiology regeneration time factor Xenopus laevis Animals Axotomy Brain-Derived Neurotrophic Factor Caspase 3 Cell Differentiation Cell Proliferation GAP-43 Protein Gene Expression Regulation Keratin-2 Nerve Degeneration Neural Cell Adhesion Molecules Olfactory Marker Protein Olfactory Mucosa Olfactory Nerve Injuries Recovery of Function Regeneration Smell Time Factors Xenopus laevis |
| spellingShingle |
neural stem cells neurodifferentiation neurogenesis olfaction olfactory receptor neurons neuromodulin neurotrophic factor neurotrophin brain derived neurotrophic factor caspase 3 cytokeratin 2 nerve cell adhesion molecule neuromodulin olfactory marker protein animal experiment animal tissue apoptosis Article axotomy basal cell cell proliferation controlled study embryo nerve cell degeneration nerve cell differentiation nerve cell necrosis nerve regeneration nervous system development nonhuman olfactory bulb olfactory epithelium olfactory nerve olfactory receptor neuron priority journal smelling Xenopus laevis animal cell differentiation convalescence gene expression regulation metabolism nerve degeneration odor olfactory mucosa olfactory nerve injury pathology physiology regeneration time factor Xenopus laevis Animals Axotomy Brain-Derived Neurotrophic Factor Caspase 3 Cell Differentiation Cell Proliferation GAP-43 Protein Gene Expression Regulation Keratin-2 Nerve Degeneration Neural Cell Adhesion Molecules Olfactory Marker Protein Olfactory Mucosa Olfactory Nerve Injuries Recovery of Function Regeneration Smell Time Factors Xenopus laevis Neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of Xenopus laevis |
| topic_facet |
neural stem cells neurodifferentiation neurogenesis olfaction olfactory receptor neurons neuromodulin neurotrophic factor neurotrophin brain derived neurotrophic factor caspase 3 cytokeratin 2 nerve cell adhesion molecule neuromodulin olfactory marker protein animal experiment animal tissue apoptosis Article axotomy basal cell cell proliferation controlled study embryo nerve cell degeneration nerve cell differentiation nerve cell necrosis nerve regeneration nervous system development nonhuman olfactory bulb olfactory epithelium olfactory nerve olfactory receptor neuron priority journal smelling Xenopus laevis animal cell differentiation convalescence gene expression regulation metabolism nerve degeneration odor olfactory mucosa olfactory nerve injury pathology physiology regeneration time factor Xenopus laevis Animals Axotomy Brain-Derived Neurotrophic Factor Caspase 3 Cell Differentiation Cell Proliferation GAP-43 Protein Gene Expression Regulation Keratin-2 Nerve Degeneration Neural Cell Adhesion Molecules Olfactory Marker Protein Olfactory Mucosa Olfactory Nerve Injuries Recovery of Function Regeneration Smell Time Factors Xenopus laevis |
| description |
The olfactory epithelium (OE) has the remarkable capability to constantly replace olfactory receptor neurons (ORNs) due to the presence of neural stem cells (NSCs). For this reason, the OE provides an excellent model to study neurogenesis and neuronal differentiation. In the present work, we induced neuronal degeneration in the OE of Xenopus laevis larvae by bilateral axotomy of the olfactory nerves. We found that axotomy induces specific- neuronal death through apoptosis between 24 and 48h post-injury. In concordance, there was a progressive decrease of the mature-ORN marker OMP until it was completely absent 72h post-injury. On the other hand, neurogenesis was evident 48h post-injury by an increase in the number of proliferating basal cells as well as NCAM-180– GAP-43+ immature neurons. Mature ORNs were replenished 21 days post-injury and the olfactory function was partially recovered, indicating that new ORNs were integrated into the olfactory bulb glomeruli. Throughout the regenerative process no changes in the expression pattern of the neurotrophin Brain Derivate Neurotrophic Factor were observed. Taken together, this work provides a sequential analysis of the neurodegenerative and subsequent regenerative processes that take place in the OE following axotomy. © 2017 Wiley Periodicals, Inc. Develop Neurobiol 77: 1308–1320, 2017. © 2017 Wiley Periodicals, Inc. |
| title |
Neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of Xenopus laevis |
| title_short |
Neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of Xenopus laevis |
| title_full |
Neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of Xenopus laevis |
| title_fullStr |
Neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of Xenopus laevis |
| title_full_unstemmed |
Neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of Xenopus laevis |
| title_sort |
neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of xenopus laevis |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19328451_v77_n11_p1308_Cervino http://hdl.handle.net/20.500.12110/paper_19328451_v77_n11_p1308_Cervino |
| _version_ |
1768546650230358016 |