The bias associated with amplicon sequencing does not affect the quantitative assessment of bacterial community dynamics

The performance of two sets of primers targeting variable regions of the 16S rRNA gene V1-V3 and V4 was compared in their ability to describe changes of bacterial diversity and temporal turnover in full-scale activated sludge. Duplicate sets of high-throughput amplicon sequencing data of the two 16S...

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Detalles Bibliográficos
Autores principales: Ibarbalz, Federico Matías, Figuerola, Eva Lucía Margarita, Erijman, Leonardo
Publicado: 2014
Materias:
RNA 16S
sewage
activated sludge
amplicon
article
community dynamics
fluorescence in situ hybridization
gene sequence
genetic variability
high throughput sequencing
microbial community
nonhuman
population abundance
quantitative analysis
taxon
temperature
Thiothrix
analysis
bacterial gene
bacterium
biodiversity
classification
DNA sequence
duplicate gene
gene amplification
genetics
growth, development and aging
microbial consortium
microbiology
procedures
RNA gene
statistical bias
statistics and numerical data
Bacteria
Bias (Epidemiology)
Biodiversity
Gene Amplification
Genes, Bacterial
Genes, Duplicate
Genes, rRNA
High-Throughput Nucleotide Sequencing
Microbial Consortia
RNA, Ribosomal, 16S
Sequence Analysis, DNA
Sewage
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v9_n6_p_Ibarbalz
http://hdl.handle.net/20.500.12110/paper_19326203_v9_n6_p_Ibarbalz
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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