Differential migration and activation profile of monocytes after trophoblast interaction
Macrophages at the maternal-placental interface coordinate opposite demands under the control of trophoblast cells such as the response against pathogens on one hand, and apoptotic cell clearance and wound healing with the production of suppressor cytokines. Here, we investigated whether trophoblast...
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2014
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v9_n5_p_Grasso http://hdl.handle.net/20.500.12110/paper_19326203_v9_n5_p_Grasso |
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paper:paper_19326203_v9_n5_p_Grasso2023-06-08T16:31:23Z Differential migration and activation profile of monocytes after trophoblast interaction B7 antigen CD14 antigen CD16 antigen CD39 antigen chemokine receptor CCR5 interleukin 10 interleukin 12 interleukin 8 lipopolysaccharide peptidoglycan polyinosinic polycytidylic acid RANTES apyrase CCL5 protein, human CCR5 protein, human CD14 antigen CD39 antigen chemokine receptor CCR5 Fc receptor FCGR3B protein, human glycosylphosphatidylinositol anchored protein interleukin 1beta leukocyte antigen lipopolysaccharide peptidoglycan polyinosinic polycytidylic acid tumor necrosis factor alpha article bacterium cell activation cell interaction cell migration coculture controlled study cytokine production female human human cell in vitro study macrophage activation monocyte placenta protein expression trophoblast virus cell communication cell motion cytology drug effects gene expression genetics metabolism monocyte trophoblast Antigens, CD Antigens, CD14 Apyrase Cell Communication Cell Movement Chemokine CCL5 Coculture Techniques Female Gene Expression GPI-Linked Proteins Humans Interleukin-1beta Interleukin-8 Lipopolysaccharides Monocytes Peptidoglycan Poly I-C Receptors, CCR5 Receptors, IgG Trophoblasts Tumor Necrosis Factor-alpha Macrophages at the maternal-placental interface coordinate opposite demands under the control of trophoblast cells such as the response against pathogens on one hand, and apoptotic cell clearance and wound healing with the production of suppressor cytokines. Here, we investigated whether trophoblast cells induce maternal monocyte activation towards an alternative activated macrophage profile and whether bacterial or viral stimuli modulate their migratory properties. We used an in vitro model of the maternal-placental interface represented by co-cultures of CD14+ cells isolated from fertile women with first trimester trophoblast cell line (Swan-71 cells) in the presence or absence of pathogen associated molecular pattern (PAMP) stimuli lipopolysaccharide (LPS), peptidoglycan (PGN) or poly [I:C]). Maternal CD14+ cells showed increased CD16 and CD39 expression, both markers associated to an alternative activation profile, with no changes in CD80 expression after trophoblast cell interaction. These changes were accompanied by increased IL-10 and decreased IL-12 production by CD14+ cells. After stimulation with LPS, PGN or poly [I:C], monocytes co-cultured with trophoblast cells had lower production of TNF-α and IL-1β compared with non co-cultured monocytes. Interestingly, monocyte migration towards trophoblast cells was prevented in the presence of LPS or PGN but not after 24h of stimulation with poly [I:C]. LPS or PGN also decreased CCR5, CXCL-8 and CCL5 expression. Finally, trophoblast cells co-cultured with monocytes in the presence of pathological stimuli failed to increase chemokine expression, indicating a bidirectional effect. In conclusion, trophoblast might 'instruct' maternal monocytes to express an alternative activation profile and restrain their early recruitment under pathological threats as one of the first strategies to avoid potential tissue damage at the maternal-placental interface. © 2014 Grasso et al. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v9_n5_p_Grasso http://hdl.handle.net/20.500.12110/paper_19326203_v9_n5_p_Grasso |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
B7 antigen CD14 antigen CD16 antigen CD39 antigen chemokine receptor CCR5 interleukin 10 interleukin 12 interleukin 8 lipopolysaccharide peptidoglycan polyinosinic polycytidylic acid RANTES apyrase CCL5 protein, human CCR5 protein, human CD14 antigen CD39 antigen chemokine receptor CCR5 Fc receptor FCGR3B protein, human glycosylphosphatidylinositol anchored protein interleukin 1beta leukocyte antigen lipopolysaccharide peptidoglycan polyinosinic polycytidylic acid tumor necrosis factor alpha article bacterium cell activation cell interaction cell migration coculture controlled study cytokine production female human human cell in vitro study macrophage activation monocyte placenta protein expression trophoblast virus cell communication cell motion cytology drug effects gene expression genetics metabolism monocyte trophoblast Antigens, CD Antigens, CD14 Apyrase Cell Communication Cell Movement Chemokine CCL5 Coculture Techniques Female Gene Expression GPI-Linked Proteins Humans Interleukin-1beta Interleukin-8 Lipopolysaccharides Monocytes Peptidoglycan Poly I-C Receptors, CCR5 Receptors, IgG Trophoblasts Tumor Necrosis Factor-alpha |
| spellingShingle |
B7 antigen CD14 antigen CD16 antigen CD39 antigen chemokine receptor CCR5 interleukin 10 interleukin 12 interleukin 8 lipopolysaccharide peptidoglycan polyinosinic polycytidylic acid RANTES apyrase CCL5 protein, human CCR5 protein, human CD14 antigen CD39 antigen chemokine receptor CCR5 Fc receptor FCGR3B protein, human glycosylphosphatidylinositol anchored protein interleukin 1beta leukocyte antigen lipopolysaccharide peptidoglycan polyinosinic polycytidylic acid tumor necrosis factor alpha article bacterium cell activation cell interaction cell migration coculture controlled study cytokine production female human human cell in vitro study macrophage activation monocyte placenta protein expression trophoblast virus cell communication cell motion cytology drug effects gene expression genetics metabolism monocyte trophoblast Antigens, CD Antigens, CD14 Apyrase Cell Communication Cell Movement Chemokine CCL5 Coculture Techniques Female Gene Expression GPI-Linked Proteins Humans Interleukin-1beta Interleukin-8 Lipopolysaccharides Monocytes Peptidoglycan Poly I-C Receptors, CCR5 Receptors, IgG Trophoblasts Tumor Necrosis Factor-alpha Differential migration and activation profile of monocytes after trophoblast interaction |
| topic_facet |
B7 antigen CD14 antigen CD16 antigen CD39 antigen chemokine receptor CCR5 interleukin 10 interleukin 12 interleukin 8 lipopolysaccharide peptidoglycan polyinosinic polycytidylic acid RANTES apyrase CCL5 protein, human CCR5 protein, human CD14 antigen CD39 antigen chemokine receptor CCR5 Fc receptor FCGR3B protein, human glycosylphosphatidylinositol anchored protein interleukin 1beta leukocyte antigen lipopolysaccharide peptidoglycan polyinosinic polycytidylic acid tumor necrosis factor alpha article bacterium cell activation cell interaction cell migration coculture controlled study cytokine production female human human cell in vitro study macrophage activation monocyte placenta protein expression trophoblast virus cell communication cell motion cytology drug effects gene expression genetics metabolism monocyte trophoblast Antigens, CD Antigens, CD14 Apyrase Cell Communication Cell Movement Chemokine CCL5 Coculture Techniques Female Gene Expression GPI-Linked Proteins Humans Interleukin-1beta Interleukin-8 Lipopolysaccharides Monocytes Peptidoglycan Poly I-C Receptors, CCR5 Receptors, IgG Trophoblasts Tumor Necrosis Factor-alpha |
| description |
Macrophages at the maternal-placental interface coordinate opposite demands under the control of trophoblast cells such as the response against pathogens on one hand, and apoptotic cell clearance and wound healing with the production of suppressor cytokines. Here, we investigated whether trophoblast cells induce maternal monocyte activation towards an alternative activated macrophage profile and whether bacterial or viral stimuli modulate their migratory properties. We used an in vitro model of the maternal-placental interface represented by co-cultures of CD14+ cells isolated from fertile women with first trimester trophoblast cell line (Swan-71 cells) in the presence or absence of pathogen associated molecular pattern (PAMP) stimuli lipopolysaccharide (LPS), peptidoglycan (PGN) or poly [I:C]). Maternal CD14+ cells showed increased CD16 and CD39 expression, both markers associated to an alternative activation profile, with no changes in CD80 expression after trophoblast cell interaction. These changes were accompanied by increased IL-10 and decreased IL-12 production by CD14+ cells. After stimulation with LPS, PGN or poly [I:C], monocytes co-cultured with trophoblast cells had lower production of TNF-α and IL-1β compared with non co-cultured monocytes. Interestingly, monocyte migration towards trophoblast cells was prevented in the presence of LPS or PGN but not after 24h of stimulation with poly [I:C]. LPS or PGN also decreased CCR5, CXCL-8 and CCL5 expression. Finally, trophoblast cells co-cultured with monocytes in the presence of pathological stimuli failed to increase chemokine expression, indicating a bidirectional effect. In conclusion, trophoblast might 'instruct' maternal monocytes to express an alternative activation profile and restrain their early recruitment under pathological threats as one of the first strategies to avoid potential tissue damage at the maternal-placental interface. © 2014 Grasso et al. |
| title |
Differential migration and activation profile of monocytes after trophoblast interaction |
| title_short |
Differential migration and activation profile of monocytes after trophoblast interaction |
| title_full |
Differential migration and activation profile of monocytes after trophoblast interaction |
| title_fullStr |
Differential migration and activation profile of monocytes after trophoblast interaction |
| title_full_unstemmed |
Differential migration and activation profile of monocytes after trophoblast interaction |
| title_sort |
differential migration and activation profile of monocytes after trophoblast interaction |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v9_n5_p_Grasso http://hdl.handle.net/20.500.12110/paper_19326203_v9_n5_p_Grasso |
| _version_ |
1768542712205672448 |