Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease
The MICA/B genes (MHC class I chain related genes A and B) encode for non conventional class I HLA molecules which have no role in antigen presentation. MICA/B are up-regulated by different stress conditions such as heat-shock, oxidative stress, neoplasic transformation and viral infection. Particul...
Guardado en:
| Publicado: |
2013
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n9_p_Allegretti http://hdl.handle.net/20.500.12110/paper_19326203_v8_n9_p_Allegretti |
| Aporte de: |
| id |
paper:paper_19326203_v8_n9_p_Allegretti |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_19326203_v8_n9_p_Allegretti2023-06-08T16:31:18Z Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease CD20 antigen CD3 antigen CD68 antigen CD7 antigen glycoprotein p 15095 major histocompatibility antigen class 1 major histocompatibility antigen class 2 syndecan 1 article B lymphocyte celiac disease cell stress cellular distribution child clinical article confocal microscopy controlled study disease severity endoplasmic reticulum stress flow cytometry gene gene expression regulation human human cell human tissue immune dysregulation immunofluorescence test immunopathogenesis intestine biopsy lamina propria macrophage MICA gene MICAB gene oxidative stress preschool child protein localization small intestine mucosa B-Lymphocytes Celiac Disease Child, Preschool Duodenum Enterocytes Female Gene Expression Histocompatibility Antigens Class I Humans Intestinal Mucosa Macrophages Male Plasma Cells Severity of Illness Index Stress, Physiological T-Lymphocytes The MICA/B genes (MHC class I chain related genes A and B) encode for non conventional class I HLA molecules which have no role in antigen presentation. MICA/B are up-regulated by different stress conditions such as heat-shock, oxidative stress, neoplasic transformation and viral infection. Particularly, MICA/B are expressed in enterocytes where they can mediate enterocyte apoptosis when recognised by the activating NKG2D receptor present on intraepithelial lymphocytes. This mechanism was suggested to play a major pathogenic role in active celiac disease (CD). Due to the importance of MICA/B in CD pathogenesis we studied their expression in duodenal tissue from CD patients. By immunofluorescence confocal microscopy and flow cytometry we established that MICA/B was mainly intracellularly located in enterocytes. In addition, we identified MICA/B+ T cells in both the intraepithelial and lamina propria compartments. We also found MICA/B+ B cells, plasma cells and some macrophages in the lamina propria. The pattern of MICA/B staining in mucosal tissue in severe enteropathy was similar to that found in in vitro models of cellular stress. In such models, MICA/B were located in stress granules that are associated to the oxidative and ER stress response observed in active CD enteropathy. Our results suggest that expression of MICA/B in the intestinal mucosa of CD patients is linked to disregulation of mucosa homeostasis in which the stress response plays an active role. © 2013 Allegretti et al. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n9_p_Allegretti http://hdl.handle.net/20.500.12110/paper_19326203_v8_n9_p_Allegretti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
CD20 antigen CD3 antigen CD68 antigen CD7 antigen glycoprotein p 15095 major histocompatibility antigen class 1 major histocompatibility antigen class 2 syndecan 1 article B lymphocyte celiac disease cell stress cellular distribution child clinical article confocal microscopy controlled study disease severity endoplasmic reticulum stress flow cytometry gene gene expression regulation human human cell human tissue immune dysregulation immunofluorescence test immunopathogenesis intestine biopsy lamina propria macrophage MICA gene MICAB gene oxidative stress preschool child protein localization small intestine mucosa B-Lymphocytes Celiac Disease Child, Preschool Duodenum Enterocytes Female Gene Expression Histocompatibility Antigens Class I Humans Intestinal Mucosa Macrophages Male Plasma Cells Severity of Illness Index Stress, Physiological T-Lymphocytes |
| spellingShingle |
CD20 antigen CD3 antigen CD68 antigen CD7 antigen glycoprotein p 15095 major histocompatibility antigen class 1 major histocompatibility antigen class 2 syndecan 1 article B lymphocyte celiac disease cell stress cellular distribution child clinical article confocal microscopy controlled study disease severity endoplasmic reticulum stress flow cytometry gene gene expression regulation human human cell human tissue immune dysregulation immunofluorescence test immunopathogenesis intestine biopsy lamina propria macrophage MICA gene MICAB gene oxidative stress preschool child protein localization small intestine mucosa B-Lymphocytes Celiac Disease Child, Preschool Duodenum Enterocytes Female Gene Expression Histocompatibility Antigens Class I Humans Intestinal Mucosa Macrophages Male Plasma Cells Severity of Illness Index Stress, Physiological T-Lymphocytes Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease |
| topic_facet |
CD20 antigen CD3 antigen CD68 antigen CD7 antigen glycoprotein p 15095 major histocompatibility antigen class 1 major histocompatibility antigen class 2 syndecan 1 article B lymphocyte celiac disease cell stress cellular distribution child clinical article confocal microscopy controlled study disease severity endoplasmic reticulum stress flow cytometry gene gene expression regulation human human cell human tissue immune dysregulation immunofluorescence test immunopathogenesis intestine biopsy lamina propria macrophage MICA gene MICAB gene oxidative stress preschool child protein localization small intestine mucosa B-Lymphocytes Celiac Disease Child, Preschool Duodenum Enterocytes Female Gene Expression Histocompatibility Antigens Class I Humans Intestinal Mucosa Macrophages Male Plasma Cells Severity of Illness Index Stress, Physiological T-Lymphocytes |
| description |
The MICA/B genes (MHC class I chain related genes A and B) encode for non conventional class I HLA molecules which have no role in antigen presentation. MICA/B are up-regulated by different stress conditions such as heat-shock, oxidative stress, neoplasic transformation and viral infection. Particularly, MICA/B are expressed in enterocytes where they can mediate enterocyte apoptosis when recognised by the activating NKG2D receptor present on intraepithelial lymphocytes. This mechanism was suggested to play a major pathogenic role in active celiac disease (CD). Due to the importance of MICA/B in CD pathogenesis we studied their expression in duodenal tissue from CD patients. By immunofluorescence confocal microscopy and flow cytometry we established that MICA/B was mainly intracellularly located in enterocytes. In addition, we identified MICA/B+ T cells in both the intraepithelial and lamina propria compartments. We also found MICA/B+ B cells, plasma cells and some macrophages in the lamina propria. The pattern of MICA/B staining in mucosal tissue in severe enteropathy was similar to that found in in vitro models of cellular stress. In such models, MICA/B were located in stress granules that are associated to the oxidative and ER stress response observed in active CD enteropathy. Our results suggest that expression of MICA/B in the intestinal mucosa of CD patients is linked to disregulation of mucosa homeostasis in which the stress response plays an active role. © 2013 Allegretti et al. |
| title |
Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease |
| title_short |
Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease |
| title_full |
Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease |
| title_fullStr |
Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease |
| title_full_unstemmed |
Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease |
| title_sort |
broad mica/b expression in the small bowel mucosa: a link between cellular stress and celiac disease |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n9_p_Allegretti http://hdl.handle.net/20.500.12110/paper_19326203_v8_n9_p_Allegretti |
| _version_ |
1768543488785252352 |