The role of Ph fronts in tissue electroporation based treatments

Treatments based on electroporation (EP) induce the formation of pores in cell membranes due to the application of pulsed electric fields. We present experimental evidence of the existence of pH fronts emerging from both electrodes during treatments based on tissue EP, for conditions found in many s...

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Detalles Bibliográficos
Publicado: 2013
Materias:
Algorithms
Animals
Dogs
Electrochemotherapy
Electroporation
Fuzzy Logic
Hydrogen-Ion Concentration
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n11_p_Maglietti
http://hdl.handle.net/20.500.12110/paper_19326203_v8_n11_p_Maglietti
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_19326203_v8_n11_p_Maglietti
record_format dspace
spelling paper:paper_19326203_v8_n11_p_Maglietti2023-06-08T16:31:12Z The role of Ph fronts in tissue electroporation based treatments Algorithms Animals Dogs Electrochemotherapy Electroporation Fuzzy Logic Hydrogen-Ion Concentration Treatments based on electroporation (EP) induce the formation of pores in cell membranes due to the application of pulsed electric fields. We present experimental evidence of the existence of pH fronts emerging from both electrodes during treatments based on tissue EP, for conditions found in many studies, and that these fronts are immediate and substantial. pH fronts are indirectly measured through the evanescence time (ET), defined as the time required for the tissue buffer to neutralize them. The ET was measured through a pH indicator imaged at a series of time intervals using a four-cluster hard fuzzy-c-means algorithm to segment pixels corresponding to the pH indicator at every frame. The ET was calculated as the time during which the number of pixels was 10% of those in the initial frame. While in EP-based treatments such as reversible (ECT) and irreversible electroporation (IRE) the ET is very short (though enough to cause minor injuries) due to electric pulse characteristics and biological buffers present in the tissue, in gene electrotransfer (GET), ET is much longer, enough to denaturate plasmids and produce cell damage. When any of the electric pulse parameters is doubled or tripled the ET grows and, remarkably, when any of the pulse parameters in GET is halved, the ET drops significantly. Reducing pH fronts has relevant implications for GET treatment efficiency, due to a substantial reduction of plasmid damage and cell loss. © 2013 Maglietti et al. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n11_p_Maglietti http://hdl.handle.net/20.500.12110/paper_19326203_v8_n11_p_Maglietti
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Algorithms
Animals
Dogs
Electrochemotherapy
Electroporation
Fuzzy Logic
Hydrogen-Ion Concentration
spellingShingle Algorithms
Animals
Dogs
Electrochemotherapy
Electroporation
Fuzzy Logic
Hydrogen-Ion Concentration
The role of Ph fronts in tissue electroporation based treatments
topic_facet Algorithms
Animals
Dogs
Electrochemotherapy
Electroporation
Fuzzy Logic
Hydrogen-Ion Concentration
description Treatments based on electroporation (EP) induce the formation of pores in cell membranes due to the application of pulsed electric fields. We present experimental evidence of the existence of pH fronts emerging from both electrodes during treatments based on tissue EP, for conditions found in many studies, and that these fronts are immediate and substantial. pH fronts are indirectly measured through the evanescence time (ET), defined as the time required for the tissue buffer to neutralize them. The ET was measured through a pH indicator imaged at a series of time intervals using a four-cluster hard fuzzy-c-means algorithm to segment pixels corresponding to the pH indicator at every frame. The ET was calculated as the time during which the number of pixels was 10% of those in the initial frame. While in EP-based treatments such as reversible (ECT) and irreversible electroporation (IRE) the ET is very short (though enough to cause minor injuries) due to electric pulse characteristics and biological buffers present in the tissue, in gene electrotransfer (GET), ET is much longer, enough to denaturate plasmids and produce cell damage. When any of the electric pulse parameters is doubled or tripled the ET grows and, remarkably, when any of the pulse parameters in GET is halved, the ET drops significantly. Reducing pH fronts has relevant implications for GET treatment efficiency, due to a substantial reduction of plasmid damage and cell loss. © 2013 Maglietti et al.
title The role of Ph fronts in tissue electroporation based treatments
title_short The role of Ph fronts in tissue electroporation based treatments
title_full The role of Ph fronts in tissue electroporation based treatments
title_fullStr The role of Ph fronts in tissue electroporation based treatments
title_full_unstemmed The role of Ph fronts in tissue electroporation based treatments
title_sort role of ph fronts in tissue electroporation based treatments
publishDate 2013
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n11_p_Maglietti
http://hdl.handle.net/20.500.12110/paper_19326203_v8_n11_p_Maglietti
_version_ 1768541819311751168

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