EphA3 expressed in the chicken tectum stimulates Nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation
Background: Retinotopic projection onto the tectum/colliculus constitutes the most studied model of topographic mapping and Eph receptors and their ligands, the ephrins, are the best characterized molecular system involved in this process. Ephrin-As, expressed in an increasing rostro-caudal gradient...
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2012
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v7_n6_p_Ortalli http://hdl.handle.net/20.500.12110/paper_19326203_v7_n6_p_Ortalli |
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paper:paper_19326203_v7_n6_p_Ortalli2023-06-08T16:31:06Z EphA3 expressed in the chicken tectum stimulates Nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation ephrin receptor A3 animal cell animal cell culture animal tissue article cell stimulation chicken concentration response controlled study embryo filopodium human human cell in vitro study in vivo study nasal retina ganglion cell nerve fiber growth nonhuman nose optic nerve fiber optic tectum protein expression protein function protein localization retina ganglion cell retinotectal projection tissue distribution Animals Axons Blotting, Western Cells, Cultured Chick Embryo Chickens Green Fluorescent Proteins HEK293 Cells Humans Immunohistochemistry Microscopy, Confocal Phosphorylation Receptor, EphA3 Retina Retinal Ganglion Cells Superior Colliculi Tectum Mesencephali Time Factors Time-Lapse Imaging Tissue Culture Techniques Tyrosine Visual Pathways Background: Retinotopic projection onto the tectum/colliculus constitutes the most studied model of topographic mapping and Eph receptors and their ligands, the ephrins, are the best characterized molecular system involved in this process. Ephrin-As, expressed in an increasing rostro-caudal gradient in the tectum/colliculus, repel temporal retinal ganglion cell (RGC) axons from the caudal tectum and inhibit their branching posterior to their termination zones. However, there are conflicting data regarding the nature of the second force that guides nasal axons to invade and branch only in the caudal tectum/colliculus. The predominant model postulates that this second force is produced by a decreasing rostro-caudal gradient of EphA7 which repels nasal optic fibers and prevents their branching in the rostral tectum/colliculus. However, as optic fibers invade the tectum/colliculus growing throughout this gradient, this model cannot explain how the axons grow throughout this repellent molecule. Methodology/Principal Findings: By using chicken retinal cultures we showed that EphA3 ectodomain stimulates nasal RGC axon growth in a concentration dependent way. Moreover, we showed that nasal axons choose growing on EphA3-expressing cells and that EphA3 diminishes the density of interstitial filopodia in nasal RGC axons. Accordingly, in vivo EphA3 ectodomain misexpression directs nasal optic fibers toward the caudal tectum preventing their branching in the rostral tectum. Conclusions: We demonstrated in vitro and in vivo that EphA3 ectodomain (which is expressed in a decreasing rostro-caudal gradient in the tectum) is necessary for topographic mapping by stimulating the nasal axon growth toward the caudal tectum and inhibiting their branching in the rostral tectum. Furthermore, the ability of EphA3 of stimulating axon growth allows understanding how optic fibers invade the tectum growing throughout this molecular gradient. Therefore, opposing tectal gradients of repellent ephrin-As and of axon growth stimulating EphA3 complement each other to map optic fibers along the rostro-caudal tectal axis. © 2012 Ortalli et al. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v7_n6_p_Ortalli http://hdl.handle.net/20.500.12110/paper_19326203_v7_n6_p_Ortalli |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
ephrin receptor A3 animal cell animal cell culture animal tissue article cell stimulation chicken concentration response controlled study embryo filopodium human human cell in vitro study in vivo study nasal retina ganglion cell nerve fiber growth nonhuman nose optic nerve fiber optic tectum protein expression protein function protein localization retina ganglion cell retinotectal projection tissue distribution Animals Axons Blotting, Western Cells, Cultured Chick Embryo Chickens Green Fluorescent Proteins HEK293 Cells Humans Immunohistochemistry Microscopy, Confocal Phosphorylation Receptor, EphA3 Retina Retinal Ganglion Cells Superior Colliculi Tectum Mesencephali Time Factors Time-Lapse Imaging Tissue Culture Techniques Tyrosine Visual Pathways |
spellingShingle |
ephrin receptor A3 animal cell animal cell culture animal tissue article cell stimulation chicken concentration response controlled study embryo filopodium human human cell in vitro study in vivo study nasal retina ganglion cell nerve fiber growth nonhuman nose optic nerve fiber optic tectum protein expression protein function protein localization retina ganglion cell retinotectal projection tissue distribution Animals Axons Blotting, Western Cells, Cultured Chick Embryo Chickens Green Fluorescent Proteins HEK293 Cells Humans Immunohistochemistry Microscopy, Confocal Phosphorylation Receptor, EphA3 Retina Retinal Ganglion Cells Superior Colliculi Tectum Mesencephali Time Factors Time-Lapse Imaging Tissue Culture Techniques Tyrosine Visual Pathways EphA3 expressed in the chicken tectum stimulates Nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation |
topic_facet |
ephrin receptor A3 animal cell animal cell culture animal tissue article cell stimulation chicken concentration response controlled study embryo filopodium human human cell in vitro study in vivo study nasal retina ganglion cell nerve fiber growth nonhuman nose optic nerve fiber optic tectum protein expression protein function protein localization retina ganglion cell retinotectal projection tissue distribution Animals Axons Blotting, Western Cells, Cultured Chick Embryo Chickens Green Fluorescent Proteins HEK293 Cells Humans Immunohistochemistry Microscopy, Confocal Phosphorylation Receptor, EphA3 Retina Retinal Ganglion Cells Superior Colliculi Tectum Mesencephali Time Factors Time-Lapse Imaging Tissue Culture Techniques Tyrosine Visual Pathways |
description |
Background: Retinotopic projection onto the tectum/colliculus constitutes the most studied model of topographic mapping and Eph receptors and their ligands, the ephrins, are the best characterized molecular system involved in this process. Ephrin-As, expressed in an increasing rostro-caudal gradient in the tectum/colliculus, repel temporal retinal ganglion cell (RGC) axons from the caudal tectum and inhibit their branching posterior to their termination zones. However, there are conflicting data regarding the nature of the second force that guides nasal axons to invade and branch only in the caudal tectum/colliculus. The predominant model postulates that this second force is produced by a decreasing rostro-caudal gradient of EphA7 which repels nasal optic fibers and prevents their branching in the rostral tectum/colliculus. However, as optic fibers invade the tectum/colliculus growing throughout this gradient, this model cannot explain how the axons grow throughout this repellent molecule. Methodology/Principal Findings: By using chicken retinal cultures we showed that EphA3 ectodomain stimulates nasal RGC axon growth in a concentration dependent way. Moreover, we showed that nasal axons choose growing on EphA3-expressing cells and that EphA3 diminishes the density of interstitial filopodia in nasal RGC axons. Accordingly, in vivo EphA3 ectodomain misexpression directs nasal optic fibers toward the caudal tectum preventing their branching in the rostral tectum. Conclusions: We demonstrated in vitro and in vivo that EphA3 ectodomain (which is expressed in a decreasing rostro-caudal gradient in the tectum) is necessary for topographic mapping by stimulating the nasal axon growth toward the caudal tectum and inhibiting their branching in the rostral tectum. Furthermore, the ability of EphA3 of stimulating axon growth allows understanding how optic fibers invade the tectum growing throughout this molecular gradient. Therefore, opposing tectal gradients of repellent ephrin-As and of axon growth stimulating EphA3 complement each other to map optic fibers along the rostro-caudal tectal axis. © 2012 Ortalli et al. |
title |
EphA3 expressed in the chicken tectum stimulates Nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation |
title_short |
EphA3 expressed in the chicken tectum stimulates Nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation |
title_full |
EphA3 expressed in the chicken tectum stimulates Nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation |
title_fullStr |
EphA3 expressed in the chicken tectum stimulates Nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation |
title_full_unstemmed |
EphA3 expressed in the chicken tectum stimulates Nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation |
title_sort |
epha3 expressed in the chicken tectum stimulates nasal retinal ganglion cell axon growth and is required for retinotectal topographic map formation |
publishDate |
2012 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v7_n6_p_Ortalli http://hdl.handle.net/20.500.12110/paper_19326203_v7_n6_p_Ortalli |
_version_ |
1768546558755733504 |