GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration
The regulation of cell migration is a highly complex process that is often compromised when cancer cells become metastatic. The microtubule cytoskeleton is necessary for cell migration, but how microtubules and microtubule-associated proteins regulate multiple pathways promoting cell migration remai...
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paper:paper_19326203_v7_n12_p_Scolz2023-06-08T16:31:03Z GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration Monte, Martín binding protein end binding 1 protein G2 and S phase expressed 1 protein microtubule associated protein microtubule plus end tracking protein microtubule protein unclassified drug article breast cancer cancer cell culture cell migration controlled study focal adhesion human human cell microtubule nucleotide sequence protein function protein protein interaction regulatory mechanism Breast Neoplasms Cell Line Cell Movement DNA Primers Female Fluorescent Antibody Technique Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Immunoprecipitation Kaplan-Meier Estimate Mass Spectrometry Microscopy, Fluorescence Microtubule-Associated Proteins Microtubules Neoplasm Invasiveness Real-Time Polymerase Chain Reaction RNA Interference RNA, Small Interfering The regulation of cell migration is a highly complex process that is often compromised when cancer cells become metastatic. The microtubule cytoskeleton is necessary for cell migration, but how microtubules and microtubule-associated proteins regulate multiple pathways promoting cell migration remains unclear. Microtubule plus-end binding proteins (+TIPs) are emerging as important players in many cellular functions, including cell migration. Here we identify a +TIP, GTSE1, that promotes cell migration. GTSE1 accumulates at growing microtubule plus ends through interaction with the EB1+TIP. The EB1-dependent +TIP activity of GTSE1 is required for cell migration, as well as for microtubule-dependent disassembly of focal adhesions. GTSE1 protein levels determine the migratory capacity of both nontransformed and breast cancer cell lines. In breast cancers, increased GTSE1 expression correlates with invasive potential, tumor stage, and time to distant metastasis, suggesting that misregulation of GTSE1 expression could be associated with increased invasive potential. © 2012 Scolz et al. Fil:Monte, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v7_n12_p_Scolz http://hdl.handle.net/20.500.12110/paper_19326203_v7_n12_p_Scolz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
binding protein end binding 1 protein G2 and S phase expressed 1 protein microtubule associated protein microtubule plus end tracking protein microtubule protein unclassified drug article breast cancer cancer cell culture cell migration controlled study focal adhesion human human cell microtubule nucleotide sequence protein function protein protein interaction regulatory mechanism Breast Neoplasms Cell Line Cell Movement DNA Primers Female Fluorescent Antibody Technique Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Immunoprecipitation Kaplan-Meier Estimate Mass Spectrometry Microscopy, Fluorescence Microtubule-Associated Proteins Microtubules Neoplasm Invasiveness Real-Time Polymerase Chain Reaction RNA Interference RNA, Small Interfering |
| spellingShingle |
binding protein end binding 1 protein G2 and S phase expressed 1 protein microtubule associated protein microtubule plus end tracking protein microtubule protein unclassified drug article breast cancer cancer cell culture cell migration controlled study focal adhesion human human cell microtubule nucleotide sequence protein function protein protein interaction regulatory mechanism Breast Neoplasms Cell Line Cell Movement DNA Primers Female Fluorescent Antibody Technique Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Immunoprecipitation Kaplan-Meier Estimate Mass Spectrometry Microscopy, Fluorescence Microtubule-Associated Proteins Microtubules Neoplasm Invasiveness Real-Time Polymerase Chain Reaction RNA Interference RNA, Small Interfering Monte, Martín GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration |
| topic_facet |
binding protein end binding 1 protein G2 and S phase expressed 1 protein microtubule associated protein microtubule plus end tracking protein microtubule protein unclassified drug article breast cancer cancer cell culture cell migration controlled study focal adhesion human human cell microtubule nucleotide sequence protein function protein protein interaction regulatory mechanism Breast Neoplasms Cell Line Cell Movement DNA Primers Female Fluorescent Antibody Technique Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Immunoprecipitation Kaplan-Meier Estimate Mass Spectrometry Microscopy, Fluorescence Microtubule-Associated Proteins Microtubules Neoplasm Invasiveness Real-Time Polymerase Chain Reaction RNA Interference RNA, Small Interfering |
| description |
The regulation of cell migration is a highly complex process that is often compromised when cancer cells become metastatic. The microtubule cytoskeleton is necessary for cell migration, but how microtubules and microtubule-associated proteins regulate multiple pathways promoting cell migration remains unclear. Microtubule plus-end binding proteins (+TIPs) are emerging as important players in many cellular functions, including cell migration. Here we identify a +TIP, GTSE1, that promotes cell migration. GTSE1 accumulates at growing microtubule plus ends through interaction with the EB1+TIP. The EB1-dependent +TIP activity of GTSE1 is required for cell migration, as well as for microtubule-dependent disassembly of focal adhesions. GTSE1 protein levels determine the migratory capacity of both nontransformed and breast cancer cell lines. In breast cancers, increased GTSE1 expression correlates with invasive potential, tumor stage, and time to distant metastasis, suggesting that misregulation of GTSE1 expression could be associated with increased invasive potential. © 2012 Scolz et al. |
| author |
Monte, Martín |
| author_facet |
Monte, Martín |
| author_sort |
Monte, Martín |
| title |
GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration |
| title_short |
GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration |
| title_full |
GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration |
| title_fullStr |
GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration |
| title_full_unstemmed |
GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration |
| title_sort |
gtse1 is a microtubule plus-end tracking protein that regulates eb1-dependent cell migration |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v7_n12_p_Scolz http://hdl.handle.net/20.500.12110/paper_19326203_v7_n12_p_Scolz |
| work_keys_str_mv |
AT montemartin gtse1isamicrotubuleplusendtrackingproteinthatregulateseb1dependentcellmigration |
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1768546743466590208 |